Cloned (Comment) | Organism |
---|---|
PAF-AH isozyme II, DNA and amino acid sequence determination and analysis, expression of wild-type enzyme in Escherichia coli, expression of isozyme II mutant enzymes in CHO-K1 cells | Rattus norvegicus |
Protein Variants | Comment | Organism |
---|---|---|
C120S | site-directed mutagenesis, residue is involved in transacetylase reaction mechanism, mutant shows 35% reduced transacetylase activity but unaltered acetylhydrolase activity | Rattus norvegicus |
G2A | site-directed mutagenesis, residue is involved in transacetylase reaction mechanism, mutant shows 50% reduced transacetylase activity but unaltered acetylhydrolase activity | Rattus norvegicus |
G2A/C120S | site-directed mutagenesis, residues are involved in transacetylase reaction mechanism, mutant shows 80% reduced transacetylase activity but unaltered acetylhydrolase activity | Rattus norvegicus |
S234C | site-directed mutagenesis, inactive mutant | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | platelet-activating factor-dependent transacetylase | Rattus norvegicus | 5829 | - |
membrane | platelet-activating factor-dependent transacetylase | Rattus norvegicus | 16020 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40000 | - |
x * 40000, cytosolic and membraneous PAF-TA, SDS-PAGE, x * 43491, PAF-AH isozyme II, amino acid sequence calculation | Rattus norvegicus |
43491 | - |
x * 40000, cytosolic and membraneous PAF-TA, SDS-PAGE, x * 43491, PAF-AH isozyme II, amino acid sequence calculation | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
2 isozymes II and Ib of platelet-activating factor acetylhydrolase PAF-AH | - |
Purification (Comment) | Organism |
---|---|
PAF-transacetylase from kidney cytosol, to homogeneity, recombinant PAF-AH isozyme II from Escherichia coli | Rattus norvegicus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
1-alkyl-2-acetyl-sn-glycero-3-phosphocholine + H2O = 1-alkyl-sn-glycero-3-phosphocholine + acetate | isozyme II of the enzyme also performs platelet-activating factor-dependent transacetylation, transfer of an acetyl group from platelet-activating factor to endogenous acceptor lipids | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
kidney | platelet-activating factor-dependent transacetylase | Rattus norvegicus | - |
lung | platelet-activating factor acetylhydrolase, isozymes II and Ib with alpha1 or alpha2 subunit | Rattus norvegicus | - |
plasma | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
PAF-AH isozyme Ib alpha1 subunit shows no transacetylase activity | Rattus norvegicus |
0.000012 | - |
PAF-AH isozyme Ib alpha2 subunit, transacetylase activity | Rattus norvegicus |
0.00028 | - |
PAF-AH isozyme Ib alpha1 subunit, acetylhydrolase activity | Rattus norvegicus |
0.00236 | - |
PAF-AH isozyme Ib alpha2 subunit, acetylhydrolase activity | Rattus norvegicus |
0.0408 | - |
PAF-AH isozyme II, transacetylase activity | Rattus norvegicus |
0.105 | - |
PAF-AH isozyme II, acetylhydrolase activity | Rattus norvegicus |
0.114 | - |
plasma PAF-AH, transacetylase activity | Rattus norvegicus |
0.131 | - |
plasma PAF-AH, acetylhydrolase activity | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-acetyl-1-alkyl-sn-glycero-3-phosphocholine + H2O | i.e. platelet-activation factor | Rattus norvegicus | 1-alkyl-sn-glycero-3-phosphocholine + acetate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 40000, cytosolic and membraneous PAF-TA, SDS-PAGE, x * 43491, PAF-AH isozyme II, amino acid sequence calculation | Rattus norvegicus |
Synonyms | Comment | Organism |
---|---|---|
More | platelet-activating factor-dependent transacetylase, PAF-TA, and platelet-activating factor acetylhydrolase isozyme II, PAF-AH II, might be the same enzyme | Rattus norvegicus |
PAF-AH | - |
Rattus norvegicus |
platelet-activating factor acetylhydrolase | - |
Rattus norvegicus |