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Literature summary for 3.1.1.47 extracted from
- Platelet-activating factor acetylhydrolase (PAF-AH) (2002), J. Biochem., 131, 635-640.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Bos taurus |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| crystal structure determination and analysis of the alpha1 subunit in complex with acetate at 1.7 A resolution | Bos taurus |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | isozyme II | Bos taurus | 5829 | - |
| intracellular | - |
Bos taurus | 5622 | - |
| membrane | isozyme II | Bos taurus | 16020 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 40000 | - |
x * 40000, isozyme II, SDS-PAGE | Bos taurus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-acetyl-1-alkyl-sn-glycero-3-phosphocholine + H2O | Mus musculus | i.e. platelet-activation factor | 1-alkyl-sn-glycero-3-phosphocholine + acetate | - |
? |  |
| 2-acetyl-1-alkyl-sn-glycero-3-phosphocholine + H2O | Homo sapiens | i.e. platelet-activation factor | 1-alkyl-sn-glycero-3-phosphocholine + acetate | - |
? | |
| 2-acetyl-1-alkyl-sn-glycero-3-phosphocholine + H2O | Rattus norvegicus | i.e. platelet-activation factor | 1-alkyl-sn-glycero-3-phosphocholine + acetate | - |
? | |
| 2-acetyl-1-alkyl-sn-glycero-3-phosphocholine + H2O | Bos taurus | biological inactivation of the platelet-activation factor by hydrolysis of the acetyl group at sn-2 position which is essential for activity of PAF | 1-alkyl-sn-glycero-3-phosphocholine + acetate | - |
? | |
| additional information | Mus musculus | substrate specificity, mammalian isozyme II possesses transacetylase activity as well | ? | - |
? | |
| additional information | Homo sapiens | substrate specificity, mammalian isozyme II possesses transacetylase activity as well | ? | - |
? | |
| additional information | Rattus norvegicus | substrate specificity, mammalian isozyme II possesses transacetylase activity as well | ? | - |
? | |
| additional information | Bos taurus | one of the physiological roles of isozyme II is to diversify the biological function of PAF by producing different lipid mediators such as analogues of PAF and C2-ceramide, mutations of the gene encoding the enzyme lead to severe diseases, e.g. brain impairment, mental retardation, or lymphoma development | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
isozymes I and II | - |
| Homo sapiens | - |
- |
- |
| Mus musculus | - |
- |
- |
| Rattus norvegicus | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| additional information | isozyme II contains a myristoylation signal at the N-terminus | Bos taurus |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| isozyme II from liver, and isozyme I from brain | Bos taurus |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| 1-alkyl-2-acetyl-sn-glycero-3-phosphocholine + H2O = 1-alkyl-sn-glycero-3-phosphocholine + acetate | mechanism | Bos taurus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| brain | neonatal and adult, isozyme I | Bos taurus | - |
| glial cell | - |
Bos taurus | - |
| liver | isozyme II | Bos taurus | - |
| additional information | distribution of hetero- and homdimer of the alpha-subunit | Bos taurus | - |
| neuron | - |
Bos taurus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-acetyl-1-alkyl-sn-glycero-3-phosphocholine + H2O | i.e. platelet-activation factor | Mus musculus | 1-alkyl-sn-glycero-3-phosphocholine + acetate | - |
? | |
| 2-acetyl-1-alkyl-sn-glycero-3-phosphocholine + H2O | i.e. platelet-activation factor | Homo sapiens | 1-alkyl-sn-glycero-3-phosphocholine + acetate | - |
? | |
| 2-acetyl-1-alkyl-sn-glycero-3-phosphocholine + H2O | i.e. platelet-activation factor | Rattus norvegicus | 1-alkyl-sn-glycero-3-phosphocholine + acetate | - |
? | |
| 2-acetyl-1-alkyl-sn-glycero-3-phosphocholine + H2O | i.e. platelet-activation factor | Bos taurus | 1-alkyl-sn-glycero-3-phosphocholine + acetate | - |
? | |
| 2-acetyl-1-alkyl-sn-glycero-3-phosphocholine + H2O | biological inactivation of the platelet-activation factor by hydrolysis of the acetyl group at sn-2 position which is essential for activity of PAF | Bos taurus | 1-alkyl-sn-glycero-3-phosphocholine + acetate | - |
? | |
| additional information | substrate specificity, mammalian isozyme II possesses transacetylase activity as well | Mus musculus | ? | - |
? | |
| additional information | substrate specificity, mammalian isozyme II possesses transacetylase activity as well | Homo sapiens | ? | - |
? | |
| additional information | substrate specificity, mammalian isozyme II possesses transacetylase activity as well | Rattus norvegicus | ? | - |
? | |
| additional information | substrate specificity, mammalian isozyme II possesses transacetylase activity as well | Bos taurus | ? | - |
? | |
| additional information | one of the physiological roles of isozyme II is to diversify the biological function of PAF by producing different lipid mediators such as analogues of PAF and C2-ceramide, mutations of the gene encoding the enzyme lead to severe diseases, e.g. brain impairment, mental retardation, or lymphoma development | Bos taurus | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 40000, isozyme II, SDS-PAGE | Bos taurus |
| More | isozyme I exists of alpha1 and alpha2 subunits, catalytic subunits, tertiary structure, as well as of a beta-subunit, the beta-subunit is a regulatory one, isozyme II is a monomer | Mus musculus |
| More | isozyme I exists of alpha1 and alpha2 subunits, catalytic subunits, tertiary structure, as well as of a beta-subunit, the beta-subunit is a regulatory one, isozyme II is a monomer | Homo sapiens |
| More | isozyme I exists of alpha1 and alpha2 subunits, catalytic subunits, tertiary structure, as well as of a beta-subunit, the beta-subunit is a regulatory one, isozyme II is a monomer | Rattus norvegicus |
| More | isozyme I exists of alpha1 and alpha2 subunits, catalytic subunits, tertiary structure, as well as of a beta-subunit, the beta-subunit is a regulatory one, isozyme II is a monomer | Bos taurus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PAF-AH | - |
Mus musculus |
| PAF-AH | - |
Homo sapiens |
| PAF-AH | - |
Rattus norvegicus |
| PAF-AH | - |
Bos taurus |
| platelet-activating factor acetylhydrolase | - |
Mus musculus |
| platelet-activating factor acetylhydrolase | - |
Homo sapiens |
| platelet-activating factor acetylhydrolase | - |
Rattus norvegicus |
| platelet-activating factor acetylhydrolase | - |
Bos taurus |
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