Literature summary for 3.1.1.47 extracted from

Sheffield, P.J.; McMullen, T.W.; Li, J.; Ho, Y.S.; Garrard, S.M.; Derewenda, U.; Derewenda, Z.S.
Preparation and crystal structure of the recombinant alpha(1)/alpha(2) catalytic heterodimer of bovine brain platelet-activating factor acetylhydrolase Ib (2001), Protein Eng., 14, 513-519.

Search for more literature for 3.1.1.47

Cloned(Commentary)

Cloned (Comment)	Organism
expression of the alpha1/alpha2 heterodimer or alpha1 homodimer as His-tagged maltose-binding fusion protein in Escherichia coli XL1-Blue using a bi-citronic expression vector	Bos taurus

Crystallization (Commentary)

Crystallization (Comment)	Organism
12 mg/ml purified recombinant alpha1/alpha2 heterodimer, hanging-drop vapour diffusion method, 21°C, 17% PEG-MME 2000, 0.1 M sodium acetate, pH 6.4, 10 mM CaCl2, X-ray diffraction structure determination and analysis at resolution 2.1 A	Bos taurus

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
intracellular	-	Bos taurus	5622	-

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
additional information	Bos taurus	enzyme plays a critical role in control of neuronal migration during cortex development	?	-	?

Organism

Organism	UniProt	Comment	Textmining
Bos taurus	Q29460	isozyme Ib	-

Purification (Commentary)

Purification (Comment)	Organism
His-tagged alpha1/alpha2 heterodimer or alpha1 homodimer as maltose-binding fusion proteins from Escherichia coli XL1-Blue, removal of tags	Bos taurus

Source Tissue

Source Tissue	Comment	Organism	Textmining
brain	-	Bos taurus	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2-acetyl-1-alkyl-sn-glycero-3-phosphocholine + H2O	i.e. platelet-activation factor	Bos taurus	1-alkyl-sn-glycero-3-phosphocholine + acetate	-	?
additional information	enzyme plays a critical role in control of neuronal migration during cortex development	Bos taurus	?	-	?

Subunits

Subunits	Comment	Organism
More	the enzyme exists as a trimer of a beta-subunit and a dimer of alpha-subunits, which can consist of a homodimer of 2 alpha1 or 2 alpha2-subunits or of a alpha1/alpha2 heterodimer	Bos taurus

Synonyms

Synonyms	Comment	Organism
PAF-AH	-	Bos taurus
platelet-activating factor acetylhydrolase	-	Bos taurus

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Release 2023.1 (January 2023)