Activating Compound | Comment | Organism | Structure |
---|---|---|---|
corticoids | regulatory function on the enzyme form from HL-60 cells | Homo sapiens | |
corticoids | regulatory function on the enzyme form from plasma | Rattus norvegicus | |
GM-CSF | regulatory function on the enzyme form from monocyte-derived macrophages | Homo sapiens | |
interleukin-beta | regulatory function on the enzyme form from plasma, liver and spleen | Cricetinae | |
lipopolysaccharides | regulatory function on the enzyme form from plasma | Mus musculus | |
lipopolysaccharides | regulatory function on the enzyme form from plasma, liver, spleen, lung, and small intestine | Cricetinae | |
M-CSF | regulatory function on the enzyme form from monocyte-derived macrophages | Homo sapiens | |
platelet-activating factor | regulatory function on the enzyme form from hepatocytes | Rattus norvegicus | |
platelet-activating factor | regulatory function on the enzyme form from HepG2 cells and monocyte-derived macrophages, no effect on erythrocytes and erythrocyte isozyme | Homo sapiens | |
progestin | regulatory function on the enzyme form from HL-60 cells | Homo sapiens | |
progestin | regulatory function on the enzyme form from plasma | Rattus norvegicus | |
tumor necrosis factor-alpha | regulatory function on the enzyme form from plasma, liver and spleen | Cricetinae | |
turpentine | regulatory function on the enzyme form from plasma | Rattus norvegicus | |
zymosan | regulatory function on the enzyme form from plasma | Rattus norvegicus |
Application | Comment | Organism |
---|---|---|
medicine | recombinant plasma enzyme form can prevent or attenuate pathological inflammation in a number of animal models, it may have pharmacological potential in human inflammatory disease as well, overview | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
- |
Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
additional information | several naturally occurring mutations of different populations are connected with diseases in humans, overview | Homo sapiens |
Q281R | naturally occurring mutation in japanes population, inactive mutant enzyme, associated with asthma | Homo sapiens |
V297F | common naturally occurring mutation in japanese population, inactive mutant enzyme, prevalence of the mutant allele is significantly higher in patients with asthma, stroke, myocardial infraction, brain hemorrhage, and nonfamilial cardiomyopathy | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
corticoids | regulatory function on the enzyme form from HL-60 cells | Homo sapiens | |
croton oil | regulatory function on the enzyme form from plasma | Oryctolagus cuniculus | |
Estrogens | regulatory function on the enzyme form from plasma | Rattus norvegicus | |
IFN-gamma | regulatory function on the enzyme form from monocyte-derived macrophages | Homo sapiens | |
interleukin-1alpha | regulatory function on the enzyme form from decidual macrophages | Homo sapiens | |
interleukin-1beta | regulatory function on the enzyme form from decidual macrophages | Homo sapiens | |
interleukin-8 | regulatory function on the enzyme form from decidual and monocyte-derived macrophages | Homo sapiens | |
lipopolysaccharides | regulatory function on the enzyme form from decidual macrophages, monocyte-derived macrophages, and HL-60 cells | Homo sapiens | |
lipopolysaccharides | regulatory function on the enzyme form from Kupffer cells, plasma, lung, liver, spleen, bile, kidney, thymus, peritoneal macrophages, and peripheral leukocytes | Rattus norvegicus | |
lyso-platelet-activating factor | regulatory function on the enzyme form from hepatocytes | Rattus norvegicus | |
tumor growth factor-beta | regulatory function on the enzyme form from HL-60 cells | Homo sapiens | |
tumor necrosis factor-alpha | regulatory function on the enzyme form from decidual macrophages | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | erythrocyte isozyme | Homo sapiens | 5829 | - |
extracellular | - |
Mus musculus | - |
- |
extracellular | - |
Oryctolagus cuniculus | - |
- |
extracellular | plasma enzyme type, secretion, physiological functions, overview | Homo sapiens | - |
- |
extracellular | plasma enzyme type, secretion, physiological functions, overview | Rattus norvegicus | - |
- |
extracellular | plasma enzyme type, secretion, physiological functions, overview | Cricetinae | - |
- |
intracellular | 4 isozymes, i.e. isozyme Ia, Ib, II, and the erythrocyte form, physiological functions, overview | Homo sapiens | 5622 | - |
intracellular | 4 isozymes, i.e. isozyme Ia, Ib, II, and the erythrocyte form, physiological functions, overview | Rattus norvegicus | 5622 | - |
intracellular | 4 isozymes, i.e. isozyme Ia, Ib, II, and the erythrocyte form, physiological functions, overview | Cricetinae | 5622 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
25000 | - |
2 * 25000, erythrocyte isozyme, SDS-PAGE | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-acetyl-1-alkyl-sn-glycero-3-phosphocholine + H2O | Homo sapiens | i.e. platelet-activation factor, hydrolysis of sn-2 ester bond of platelet-activating factor PAF and related inflammatory phospholipids, attenuation of their bioactivity, the beta-subunit of intracellular isozyme type II is encoded by gene LIS1, mutations of which cause Miller-Dieker lissencephaly | 1-alkyl-sn-glycero-3-phosphocholine + acetate | - |
? | |
2-acetyl-1-alkyl-sn-glycero-3-phosphocholine + H2O | Cricetinae | i.e. platelet-activation factor, reaction leads to inactivation of the factor and therefore inhibits the initiation of inflammation processes | 1-alkyl-sn-glycero-3-phosphocholine + acetate | - |
? | |
2-acetyl-1-alkyl-sn-glycero-3-phosphocholine + H2O | Mus musculus | i.e. platelet-activation factor, reaction leads to inactivation of the factor and therefore inhibitsthe initiation of inflammation processes | 1-alkyl-sn-glycero-3-phosphocholine + acetate | - |
? | |
2-acetyl-1-alkyl-sn-glycero-3-phosphocholine + H2O | Rattus norvegicus | i.e. platelet-activation factor, reaction leads to inactivation of the factor and therefore inhibitsthe initiation of inflammation processes | 1-alkyl-sn-glycero-3-phosphocholine + acetate | - |
? | |
2-acetyl-1-alkyl-sn-glycero-3-phosphocholine + H2O | Oryctolagus cuniculus | i.e. platelet-activation factor, reaction leads to inactivation of the factor and therefore inhibitsthe initiation of inflammation processes | 1-alkyl-sn-glycero-3-phosphocholine + acetate | - |
? | |
2-acetyl-1-O-alkyl-sn-glycero-3-phosphoric acid + H2O | Mus musculus | - |
1-O-alkyl-sn-glycero-3-phosphoric acid + acetate | - |
? | |
2-acetyl-1-O-alkyl-sn-glycero-3-phosphoric acid + H2O | Homo sapiens | - |
1-O-alkyl-sn-glycero-3-phosphoric acid + acetate | - |
? | |
2-acetyl-1-O-alkyl-sn-glycero-3-phosphoric acid + H2O | Rattus norvegicus | - |
1-O-alkyl-sn-glycero-3-phosphoric acid + acetate | - |
? | |
2-acetyl-1-O-alkyl-sn-glycero-3-phosphoric acid + H2O | Oryctolagus cuniculus | - |
1-O-alkyl-sn-glycero-3-phosphoric acid + acetate | - |
? | |
2-acetyl-1-O-alkyl-sn-glycero-3-phosphoric acid + H2O | Cricetinae | - |
1-O-alkyl-sn-glycero-3-phosphoric acid + acetate | - |
? | |
additional information | Homo sapiens | enzyme level in vivo is linked to the LDL-level, physiological functions of isozymes, overview, the beta-subunit of isozyme Ib interacts with tubulin and with microtubule-associated proteins | ? | - |
? | |
additional information | Mus musculus | physiological functions of isozymes, overview | ? | - |
? | |
additional information | Rattus norvegicus | physiological functions of isozymes, overview | ? | - |
? | |
additional information | Oryctolagus cuniculus | physiological functions of isozymes, overview | ? | - |
? | |
additional information | Cricetinae | physiological functions of isozymes, overview | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cricetinae | - |
- |
- |
Homo sapiens | - |
1 extracellular, secreted plasma enzyme form and an intracellular form, devided into 4 isozyme types, differing in their primary sequences, tissue localizations, subunit compositions, and substrate preferences | - |
Mus musculus | - |
- |
- |
Oryctolagus cuniculus | - |
- |
- |
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
the erythrocyte isozyme from the soluble fraction of erythrocytes | Homo sapiens |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
1-alkyl-2-acetyl-sn-glycero-3-phosphocholine + H2O = 1-alkyl-sn-glycero-3-phosphocholine + acetate | the catalytic triad of the extracellular plasma enzyme form is of the alpha/beta-hydrolase type | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
bile | - |
Rattus norvegicus | - |
brain | - |
Homo sapiens | - |
brain | - |
Rattus norvegicus | - |
erythrocyte | erythrocyte isozyme | Homo sapiens | - |
Hep-G2 cell | - |
Homo sapiens | - |
hepatocyte | - |
Rattus norvegicus | - |
HL-60 cell | - |
Homo sapiens | - |
kidney | - |
Rattus norvegicus | - |
Kupffer cell | - |
Rattus norvegicus | - |
leukocyte | peripheral | Rattus norvegicus | - |
liver | - |
Homo sapiens | - |
liver | - |
Rattus norvegicus | - |
lung | - |
Rattus norvegicus | - |
macrophage | peritoneal | Rattus norvegicus | - |
macrophage | decidual and monocyte-derived | Homo sapiens | - |
plasma | - |
Oryctolagus cuniculus | - |
plasma | - |
Cricetinae | - |
plasma | plasma isozyme is not associated with low-density lipoprotein LDL, but with high-density lipoprotein HDL | Mus musculus | - |
plasma | plasma isozyme is not associated with low-density lipoprotein LDL, but with high-density lipoprotein HDL | Rattus norvegicus | - |
plasma | plasma isozyme, two third or more of the enzymatic activity is associated with low-density lipoprotein LDL, involving Trp115, Leu116, and Tyr205, the remainder is associated with high-density lipoprotein HDL | Homo sapiens | - |
spleen | - |
Rattus norvegicus | - |
thymus | - |
Rattus norvegicus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-acetyl-1-alkyl-sn-glycero-3-phosphocholine + H2O | i.e. platelet-activation factor | Mus musculus | 1-alkyl-sn-glycero-3-phosphocholine + acetate | - |
? | |
2-acetyl-1-alkyl-sn-glycero-3-phosphocholine + H2O | i.e. platelet-activation factor | Rattus norvegicus | 1-alkyl-sn-glycero-3-phosphocholine + acetate | - |
? | |
2-acetyl-1-alkyl-sn-glycero-3-phosphocholine + H2O | i.e. platelet-activation factor | Oryctolagus cuniculus | 1-alkyl-sn-glycero-3-phosphocholine + acetate | - |
? | |
2-acetyl-1-alkyl-sn-glycero-3-phosphocholine + H2O | i.e. platelet-activation factor | Cricetinae | 1-alkyl-sn-glycero-3-phosphocholine + acetate | - |
? | |
2-acetyl-1-alkyl-sn-glycero-3-phosphocholine + H2O | i.e. platelet-activation factor, hydrolysis of sn-2 ester bond of platelet-activating factor PAF and related inflammatory phospholipids, attenuation of their bioactivity, the beta-subunit of intracellular isozyme type II is encoded by gene LIS1, mutations of which cause Miller-Dieker lissencephaly | Homo sapiens | 1-alkyl-sn-glycero-3-phosphocholine + acetate | - |
? | |
2-acetyl-1-alkyl-sn-glycero-3-phosphocholine + H2O | i.e. platelet-activation factor, reaction leads to inactivation of the factor and therefore inhibits the initiation of inflammation processes | Cricetinae | 1-alkyl-sn-glycero-3-phosphocholine + acetate | - |
? | |
2-acetyl-1-alkyl-sn-glycero-3-phosphocholine + H2O | i.e. platelet-activation factor, reaction leads to inactivation of the factor and therefore inhibitsthe initiation of inflammation processes | Mus musculus | 1-alkyl-sn-glycero-3-phosphocholine + acetate | - |
? | |
2-acetyl-1-alkyl-sn-glycero-3-phosphocholine + H2O | i.e. platelet-activation factor, reaction leads to inactivation of the factor and therefore inhibitsthe initiation of inflammation processes | Rattus norvegicus | 1-alkyl-sn-glycero-3-phosphocholine + acetate | - |
? | |
2-acetyl-1-alkyl-sn-glycero-3-phosphocholine + H2O | i.e. platelet-activation factor, reaction leads to inactivation of the factor and therefore inhibitsthe initiation of inflammation processes | Oryctolagus cuniculus | 1-alkyl-sn-glycero-3-phosphocholine + acetate | - |
? | |
2-acetyl-1-O-alkyl-sn-glycero-3-phosphocholine + H2O | i.e. platelet-activation factor, hydrolysis of sn-2 ester bond of platelet-activating factor PAF and related inflammatory phospholipids, no activity with the erythrocyte isozyme | Homo sapiens | 1-O-alkyl-sn-glycero-3-phosphocholine + acetate | - |
? | |
2-acetyl-1-O-alkyl-sn-glycero-3-phosphoethanolamine + H2O | - |
Homo sapiens | 1-O-alkyl-sn-glycero-3-phosphoethanolamine + acetate | - |
? | |
2-acetyl-1-O-alkyl-sn-glycero-3-phosphoric acid + H2O | - |
Mus musculus | 1-O-alkyl-sn-glycero-3-phosphoric acid + acetate | - |
? | |
2-acetyl-1-O-alkyl-sn-glycero-3-phosphoric acid + H2O | - |
Homo sapiens | 1-O-alkyl-sn-glycero-3-phosphoric acid + acetate | - |
? | |
2-acetyl-1-O-alkyl-sn-glycero-3-phosphoric acid + H2O | - |
Rattus norvegicus | 1-O-alkyl-sn-glycero-3-phosphoric acid + acetate | - |
? | |
2-acetyl-1-O-alkyl-sn-glycero-3-phosphoric acid + H2O | - |
Oryctolagus cuniculus | 1-O-alkyl-sn-glycero-3-phosphoric acid + acetate | - |
? | |
2-acetyl-1-O-alkyl-sn-glycero-3-phosphoric acid + H2O | - |
Cricetinae | 1-O-alkyl-sn-glycero-3-phosphoric acid + acetate | - |
? | |
additional information | substrate specificity of the enzyme forms and isoenzyme types | Mus musculus | ? | - |
? | |
additional information | substrate specificity of the enzyme forms and isoenzyme types | Rattus norvegicus | ? | - |
? | |
additional information | substrate specificity of the enzyme forms and isoenzyme types | Oryctolagus cuniculus | ? | - |
? | |
additional information | substrate specificity of the enzyme forms and isoenzyme types | Cricetinae | ? | - |
? | |
additional information | substrate specificity of the enzyme forms and isoenzyme types, the isozyme Ib interacts with some plekstrin domains in vitro | Homo sapiens | ? | - |
? | |
additional information | enzyme level in vivo is linked to the LDL-level, physiological functions of isozymes, overview, the beta-subunit of isozyme Ib interacts with tubulin and with microtubule-associated proteins | Homo sapiens | ? | - |
? | |
additional information | physiological functions of isozymes, overview | Mus musculus | ? | - |
? | |
additional information | physiological functions of isozymes, overview | Rattus norvegicus | ? | - |
? | |
additional information | physiological functions of isozymes, overview | Oryctolagus cuniculus | ? | - |
? | |
additional information | physiological functions of isozymes, overview | Cricetinae | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 25000, erythrocyte isozyme, SDS-PAGE | Homo sapiens |
monomer | intracellular isozyme II, and plasma enzyme form | Mus musculus |
monomer | intracellular isozyme II, and plasma enzyme form | Homo sapiens |
monomer | intracellular isozyme II, and plasma enzyme form | Rattus norvegicus |
monomer | intracellular isozyme II, and plasma enzyme form | Oryctolagus cuniculus |
monomer | intracellular isozyme II, and plasma enzyme form | Cricetinae |
trimer | the intracellular G-protein-like isozyme Ib consists of two catalytic subunits alpha1 and alpha2 and one regulatory subunit beta | Mus musculus |
trimer | the intracellular G-protein-like isozyme Ib consists of two catalytic subunits alpha1 and alpha2 and one regulatory subunit beta | Homo sapiens |
trimer | the intracellular G-protein-like isozyme Ib consists of two catalytic subunits alpha1 and alpha2 and one regulatory subunit beta | Rattus norvegicus |
trimer | the intracellular G-protein-like isozyme Ib consists of two catalytic subunits alpha1 and alpha2 and one regulatory subunit beta | Oryctolagus cuniculus |
trimer | the intracellular G-protein-like isozyme Ib consists of two catalytic subunits alpha1 and alpha2 and one regulatory subunit beta | Cricetinae |
Synonyms | Comment | Organism |
---|---|---|
PAF acetylhydrolase | - |
Mus musculus |
PAF acetylhydrolase | - |
Homo sapiens |
PAF acetylhydrolase | - |
Rattus norvegicus |
PAF acetylhydrolase | - |
Oryctolagus cuniculus |
PAF acetylhydrolase | - |
Cricetinae |
platelet-activating factor acetylhydrolase | - |
Mus musculus |
platelet-activating factor acetylhydrolase | - |
Homo sapiens |
platelet-activating factor acetylhydrolase | - |
Rattus norvegicus |
platelet-activating factor acetylhydrolase | - |
Oryctolagus cuniculus |
platelet-activating factor acetylhydrolase | - |
Cricetinae |