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3.1.1.43: alpha-amino-acid esterase

This is an abbreviated version!
For detailed information about alpha-amino-acid esterase, go to the full flat file.

Word Map on EC 3.1.1.43

Reaction

an alpha-amino acid ester
+
H2O
=
an alpha-amino acid
+
an alcohol

Synonyms

AEH, alpha-amino acid ester esterase, alpha-amino acid ester hydrolase, biphenyl hydrolase-like protein, BPH, esterase, alpha-amino acid, VACVase, valacyclovirase

ECTree

     3 Hydrolases
         3.1 Acting on ester bonds
             3.1.1 Carboxylic-ester hydrolases
                3.1.1.43 alpha-amino-acid esterase

Purification

Purification on EC 3.1.1.43 - alpha-amino-acid esterase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
ammonium sulfate, DEAE-Toyopearl, electrofocusing, Disc electrophoresis
-
CM-Sepharose, hydrophobic interaction, gel filtration
from liver
-
metal ion affinity chromatography using nickel-nitrilotriacetic acid-Agarose
recombinant AEH
recombinant AEH, Ni2+-agarose, gel filtration
-
recombinant His6-tagged hVACVase construct from Escherichia coli by affinity chromatography
-
using a S strong cation exchange and a phenyl-Sepharose hydrophobic interaction column
-