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Literature summary for 3.1.1.43 extracted from

  • Barends, T.R.; Polderman-Tijmes, J.J.; Jekel, P.A.; Hensgens, C.M.; de Vries, E.J.; Janssen, D.B.; Dijkstra, B.W.
    The sequence and crystal structure of the alpha-amino acid ester hydrolase from Xanthomonas citri define a new family of beta-lactam antibiotic acylases (2003), J. Biol. Chem., 278, 23076-23084.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Xanthomonas citri

Crystallization (Commentary)

Crystallization (Comment) Organism
0.0015 ml of concentrated AEH is mixed with an equal volume of 12-15% polyethylene glycol 8000 in 100 mM cacodylate, pH 6.5, and equilibrated against 0.5 ml of this precipitant solution, crystals diffract to 1.9A Xanthomonas citri

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
70915
-
4 * 70915, deduced from nucleotide sequence Xanthomonas citri
72000
-
4 * 72000 Xanthomonas citri

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
an alpha-aminoacid ester + H2O Xanthomonas citri
-
an alpha-amino acid + an alcohol
-
r

Organism

Organism UniProt Comment Textmining
Xanthomonas citri
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant AEH Xanthomonas citri

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
an alpha-aminoacid ester + H2O
-
Xanthomonas citri an alpha-amino acid + an alcohol
-
?
an alpha-aminoacid ester + H2O
-
Xanthomonas citri an alpha-amino acid + an alcohol
-
r

Subunits

Subunits Comment Organism
tetramer 4 * 70915, deduced from nucleotide sequence Xanthomonas citri
tetramer 4 * 72000 Xanthomonas citri

Synonyms

Synonyms Comment Organism
AEH
-
Xanthomonas citri