3.1.1.43: alpha-amino-acid esterase

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For detailed information about alpha-amino-acid esterase, go to the full flat file.

Reaction

Synonyms

AEH, alpha-amino acid ester esterase, alpha-amino acid ester hydrolase, biphenyl hydrolase-like protein, BPH, esterase, alpha-amino acid, VACVase, valacyclovirase

ECTree

     3 Hydrolases

         3.1 Acting on ester bonds

             3.1.1 Carboxylic-ester hydrolases

                3.1.1.43 alpha-amino-acid esterase

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Results	in table
1	Activating Compound
20	AA Sequence
24	Application
1	CAS Registry Number
12	Cloned(Commentary)
4	Crystallization (Commentary)
3	Disease/ Diagnostics
57	General Information
1	General Stability
15	Inhibitors
35	kcat/KM [mM/s]
76	KM Value [mM]
1	Localization
3	Metals/Ions
23	Molecular Weight [Da]
12	Natural Substrates/ Products (Substrates)
22	Organic Solvent Stability
63	Organism
36	PDB ID
27	pH Optimum
2	pH Range
2	pH Stability
9	pI Value
1	Posttranslational Modification
24	Protein Variants
12	Purification (Commentary)
2	Reaction
4	Reaction Type
59	Reference
10	Source Tissue
19	Specific Activity [micromol/min/mg]
266	Substrates and Products (Substrate)
34	Subunits
89	Synonyms
1	Systematic Name
23	Temperature Optimum [°C]
2	Temperature Range [°C]
3	Temperature Stability [°C]
54	Turnover Number [1/s]

Cloned

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Cloned on EC 3.1.1.43 - alpha-amino-acid esterase

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CLONED (Commentary)

ORGANISM

UNIPROT

LITERATURE

-

Acetobacter pasteurianus

-

649217

expression in Escherichia coli

2 entries

expression of His-tagged AEH in Escherichia coli

Acetobacter pasteurianus

-

652282

gene aehA, expression in Escherichia coli

Acetobacter pasteurianus

-

729021

gene aehR, expression in Escherichia coli

Acidovorax avenae

-

729021

gene aehX, expression in Escherichia coli

Xanthomonas citri

-

729021

gene gaa, expression in Escherichia coli

Xanthomonas campestris

-

729021

into the vector pET17b for expression in Escherichia coli BL21DE3 and B834DE3 cells

Homo sapiens

-

693224

overexpression of wild-type and mutant enzymes in Escherichia coli

Acidovorax avenae

-

729461

recombinant expression in Escherichia coli strain VKPM B-11246

Acidovorax avenae

-

730436

recombinant expression of the His6-tagged hVACVase construct, M-S tag-PDLGTLVPRGSMGM-hVACVase-AAALE-His tag, in Escherichia coli

Homo sapiens

-

716273

expression in Escherichia coli

Xanthomonas citri

-

652406

expression in Escherichia coli

Acetobacter pasteurianus

Q8VRK8

649372