3.1.1.43: alpha-amino-acid esterase
This is an abbreviated version!
For detailed information about alpha-amino-acid esterase, go to the full flat file.
Word Map on EC 3.1.1.43
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3.1.1.43
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prodrugs
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synthesis
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acetobacter
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turbidans
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xanthomonas
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ampicillin
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valganciclovir
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cephalexin
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promoiety
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beta-lactam
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carrier-mediated
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cocaine
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citri
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alpha/beta-hydrolase
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acylases
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drug development
- 3.1.1.43
-
prodrugs
- synthesis
-
acetobacter
- turbidans
- xanthomonas
- ampicillin
- valganciclovir
- cephalexin
-
promoiety
- beta-lactam
-
carrier-mediated
- cocaine
- citri
-
alpha/beta-hydrolase
- acylases
- drug development
Reaction
Synonyms
AEH, alpha-amino acid ester esterase, alpha-amino acid ester hydrolase, biphenyl hydrolase-like protein, BPH, esterase, alpha-amino acid, VACVase, valacyclovirase
ECTree
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Engineering
Engineering on EC 3.1.1.43 - alpha-amino-acid esterase
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S205A
Y206A
V131S
Y206A
additional information
Y206A
active site mutant with an increased tendency to catalyze antibiotic production rather than hydrolysis
V131S
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site-directed saturation mutagenesis, the mutant shows the highest initial rates of synthesis toward three p-hydroxyl cephalosporins with 23%, 17% and 64% increases in maximum product accumulation of cefadroxil, cefprozil and cefatrizine, respectively
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based on the crystal structure of the AEH and cefprozil complex, 13 residues not directly involved in substrate recognition are mutated individually, mutant screening for activity using cefprozil as a model product, overview
additional information
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synthesis of amino-betalactam antibiotic cephalexin by the recombinant enzyme produced in Escherichia coli VKPM B-11246 is more efficient in comparison with the wild-type enzyme prepared from mutant strain Xanthomonas rubrilineans VKPM B-9915. Addition of ethylene glycol at 33 vol% to the reaction medium for cephalexin synthesis improves the yield from 70% to 95%
additional information
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synthesis of amino-betalactam antibiotic cephalexin by the recombinant enzyme produced in Escherichia coli VKPM B-11246 is more efficient in comparison with the wild-type enzyme prepared from mutant strain Xanthomonas rubrilineans VKPM B-9915. Addition of ethylene glycol at 33 vol% to the reaction medium for cephalexin synthesis improves the yield from 70% to 95%
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additional information
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Xanthomonas citri strain K24, a beta-lactamase deficient IFO 3835 mutant strain is active at pH 6.0-7.0 in cephalexin synthesis in 5% vol 2-butanol
additional information
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Xanthomonas citri strain K24, a beta-lactamase deficient IFO 3835 mutant strain is active at pH 6.0-7.0 in cephalexin synthesis in 5% vol 2-butanol
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additional information
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Xanthomonas citri strain K24, a beta-lactamase deficient IFO 3835 mutant strain is active at pH 6.0-7.0 in cephalexin synthesis in 5% vol 2-butanol
additional information
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Xanthomonas citri strain K24, a beta-lactamase deficient IFO 3835 mutant strain is active at pH 6.0-7.0 in cephalexin synthesis in 5% vol 2-butanol
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