Crystallization (Comment) | Organism |
---|---|
- |
Acetobacter pasteurianus |
Protein Variants | Comment | Organism |
---|---|---|
S205A | inactive mutant | Acetobacter pasteurianus |
Y206A | active site mutant with an increased tendency to catalyze antibiotic production rather than hydrolysis | Acetobacter pasteurianus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.45 | - |
cephalexin | - |
Acetobacter pasteurianus | |
1 | - |
D-phenylglycine methyl ester | - |
Acetobacter pasteurianus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acetobacter pasteurianus | Q8VRK8 | - |
- |
Purification (Comment) | Organism |
---|---|
metal ion affinity chromatography using nickel-nitrilotriacetic acid-Agarose | Acetobacter pasteurianus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-phenylglycine methyl ester + 7-amino-desacetoxycephalosporanic acid | - |
Acetobacter pasteurianus | cephalexin + H2O | - |
r |
Subunits | Comment | Organism |
---|---|---|
tetramer | - |
Acetobacter pasteurianus |
Synonyms | Comment | Organism |
---|---|---|
alpha-amino acid ester hydrolase | - |
Acetobacter pasteurianus |