Inhibitors | Comment | Organism | Structure |
---|---|---|---|
phenylmethanesulfonyl fluoride | 1 mM, 99% inhibition | Bacillus mycoides |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
39000 | - |
4 * 39000, SDS-PAGE | Bacillus mycoides |
160000 | - |
gel filtration | Bacillus mycoides |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
an alpha-aminoacid ester + H2O | Bacillus mycoides | - |
an alpha-amino acid + an alcohol | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus mycoides | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate, DEAE-Toyopearl, electrofocusing, Disc electrophoresis | Bacillus mycoides |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2160 | - |
- |
Bacillus mycoides |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-D-phenyl methyl ester + L-leucine amide | - |
Bacillus mycoides | acetyl-D-phenyl-L-leucine + methanol | - |
? | |
acetyl-D-phenyl methyl ester + L-phenylalanine amide | - |
Bacillus mycoides | acetyl-D-phenyl-L-phenylalanine + methanol | - |
? | |
acetyl-D-phenylalanine methyl ester + H2O | - |
Bacillus mycoides | acetyl-D-phenylalanine + methanol | - |
r | |
acetyl-L-phenylalanine methyl ester + H2O | - |
Bacillus mycoides | acetyl-L-phenylalanine + methanol | - |
r | |
an alpha-aminoacid ester + H2O | - |
Bacillus mycoides | an alpha-amino acid + an alcohol | - |
? | |
an alpha-aminoacid ester + H2O | - |
Bacillus mycoides | an alpha-amino acid + an alcohol | - |
r | |
D-aspartate dimethyl ester + H2O | - |
Bacillus mycoides | D-aspartate + methanol | - |
r | |
D-methionine methyl ester + H2O | - |
Bacillus mycoides | D-methionine + methanol | - |
r | |
D-phenylalanine amide + H2O | - |
Bacillus mycoides | D-phenylalanine + NH3 | - |
? | |
D-phenylalanine methyl ester + H2O | - |
Bacillus mycoides | D-phenylalanine + methanol | - |
r | |
D-tryptophan methyl ester + H2O | - |
Bacillus mycoides | D-tryptophan + methanol | - |
r | |
D-tyrosine methyl ester + H2O | - |
Bacillus mycoides | D-tyrosine + methanol | - |
r | |
L-aspartate dimethyl ester + H2O | - |
Bacillus mycoides | L-aspartate + methanol | - |
? | |
L-methionine methyl ester + H2O | - |
Bacillus mycoides | L-methionine + methanol | - |
r | |
L-phenylalanine methyl ester + H2O | - |
Bacillus mycoides | L-phenylalanine + methanol | - |
r | |
L-tryptophan methyl ester + H2O | - |
Bacillus mycoides | L-tryptophan + methanol | - |
r | |
L-tyrosine methyl ester + H2O | - |
Bacillus mycoides | L-tyrosine + methanol | - |
r | |
methyl acetate | 1.1% of activity with D-phenylalanine methylester | Bacillus mycoides | acetate + methanol | - |
? | |
methyl butyrate | 3.6% of activity with D-phenylalanine methylester | Bacillus mycoides | butyrate + methanol | - |
? | |
methyl caproate | 2% of activity with D-phenylalanine methylester | Bacillus mycoides | caproate + methanol | - |
? | |
methyl caprylate | 1.1% of activity with D-phenylalanine methylester | Bacillus mycoides | caprylate + methanol | - |
? | |
methyl propionate | 2.7% of activity with D-phenylalanine methylester | Bacillus mycoides | propionate + methanol | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 39000, SDS-PAGE | Bacillus mycoides |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
47 | - |
- |
Bacillus mycoides |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
10 | 65 | 40% of maximal activity at 10°C, approx. 5% of maximal activity at 65°C | Bacillus mycoides |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
6160 | - |
phenylalanine methylester | 25°C, pH 7.0 | Bacillus mycoides |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.6 | - |
hydrolysis of phenylalanine methylester | Bacillus mycoides |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 11 | approx. 10% of maximal activity at pH 4.0 and pH 10.0, respectively | Bacillus mycoides |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Bacillus mycoides | isoelectric focusing | - |
7 |