1.4.3.3: D-amino-acid oxidase

This is an abbreviated version!
For detailed information about D-amino-acid oxidase, go to the full flat file.

Word Map on EC 1.4.3.3

Reaction

a D-amino acid
+
H2O
+
O2
=
a 2-oxo carboxylate
+
NH3
+
H2O2

Synonyms

chDAO, D-AAO, D-amino acid oxidase, D-amino acid oxidases, D-amino-acid-oxidase, D-aminoacid oxidase, DAAO, DAMOX, DAO, DAO1, DaoE, hDAAO, ophio-amino-acid oxidase, oxidase, D-amino acid, PEG-DAO, pkDAAO, RgDAAO, TvDAAO, TvDAO,  LH99

ECTree

     1 Oxidoreductases
         1.4 Acting on the CH-NH2 group of donors
             1.4.3 With oxygen as acceptor
                1.4.3.3 D-amino-acid oxidase

KCat KM Value

KCat KM Value on EC 1.4.3.3 - D-amino-acid oxidase

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kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
3.83 - 18.33
cephalosporin C
6.5 - 2165
D-Ala
0.2 - 49.7
D-alanine
2802
D-Arg
-
pH 8.3, 37°C, recombinant DAO
0.59 - 14.8
D-Asn
0.003
D-Aspartate
-
at pH 7.0 and 25°C
14.5
D-Dopa
-
at pH 7.0 and 25°C
521
D-His
-
pH 8.3, 37°C, recombinant DAO
442
D-Ile
at pH 8.0 and 60°C
25 - 490
D-Leu
0.12
D-Lys
-
wild type enzyme, in 50 mM potassium phosphate buffer, pH 8.0, at 30°C
12.7 - 175
D-Met
153 - 274
D-methionine
37 - 9215
D-Phe
0.24 - 2.4
D-phenylalanine
0.55 - 1.3
D-proline
0.56 - 75
D-Ser
0.02 - 0.4
D-serine
0.16 - 0.931
D-Thr
12 - 86.5
D-Trp
0.25 - 2.1
D-tryptophan
50 - 269
D-Tyr
9.9
D-tyrosine
-
at pH 7.0 and 25°C
0.25 - 334
D-Val
0.005
glycine
-
at pH 7.0 and 25°C