1.4.3.3: D-amino-acid oxidase

This is an abbreviated version!
For detailed information about D-amino-acid oxidase, go to the full flat file.

Word Map on EC 1.4.3.3

Reaction

a D-amino acid
+
H2O
+
O2
=
a 2-oxo carboxylate
+
NH3
+
H2O2

Synonyms

chDAO, D-AAO, D-amino acid oxidase, D-amino acid oxidases, D-amino-acid-oxidase, D-aminoacid oxidase, DAAO, DAMOX, DAO, DAO1, DaoE, hDAAO, ophio-amino-acid oxidase, oxidase, D-amino acid, PEG-DAO, pkDAAO, RgDAAO, TvDAAO, TvDAO, LH99

ECTree

     1 Oxidoreductases
         1.4 Acting on the CH-NH2 group of donors
             1.4.3 With oxygen as acceptor
                1.4.3.3 D-amino-acid oxidase

pH Stability

pH Stability on EC 1.4.3.3 - D-amino-acid oxidase

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pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
10.5
-
remains fully stable up to pH 10.5
684631
4 - 11
5 - 10
5 - 8
the enzyne is stable at a pH 5.0 to 8.0 for 1 h at 55C
741688
5.4 - 8.5
-
the response to 0.01 mM D-serine increases from pH 5.4 to 8.0, reaches a maximum at pH 8.0-8.5, and decreases at higher values, the response at physiological pH (7.4) is 80.7% of the maximum response observed at pH 8.5
684417
5.5 - 11
-
1 h, stable
655274
5.5 - 9
-
25C, 24 h, stable, wild-type enzyme and mutant enzyme F42C
672703
6 - 10
-
-
713128
6 - 8
6 - 8.2
6 - 9
7.3 - 7.9
-
30 min stable
391833
8
-
unstable above
391852
8 - 9
-
at pH 8 immobilized enzyme (onto Ni2+-chelated NTA magnetic beads) activity decreases to 44% while the free enzyme showed optimal activity, the activities of both enzyme forms decay similarily at pH values above 9, the free enzyme activity is less affected for pH values below 9 than the immobilized enzyme
687855