1.4.3.3: D-amino-acid oxidase

This is an abbreviated version!
For detailed information about D-amino-acid oxidase, go to the full flat file.

Word Map on EC 1.4.3.3

Reaction

a D-amino acid
+
H2O
+
O2
=
a 2-oxo carboxylate
+
NH3
+
H2O2

Synonyms

chDAO, D-AAO, D-amino acid oxidase, D-amino acid oxidases, D-amino-acid-oxidase, D-aminoacid oxidase, DAAO, DAMOX, DAO, DAO1, DaoE, hDAAO, ophio-amino-acid oxidase, oxidase, D-amino acid, PEG-DAO, pkDAAO, RgDAAO, TvDAAO, TvDAO,  LH99

ECTree

     1 Oxidoreductases
         1.4 Acting on the CH-NH2 group of donors
             1.4.3 With oxygen as acceptor
                1.4.3.3 D-amino-acid oxidase

pH Stability

pH Stability on EC 1.4.3.3 - D-amino-acid oxidase

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pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4 - 11
5 - 10
5 - 8
the enzyne is stable at a pH 5.0 to 8.0 for 1 h at 55°C
741688
5 - 10
-
retains more than 80% of the maximal activity after incubation for 60 min at pH 5-10
685750
5.4 - 8.5
-
the response to 0.01 mM D-serine increases from pH 5.4 to 8.0, reaches a maximum at pH 8.0-8.5, and decreases at higher values, the response at physiological pH (7.4) is 80.7% of the maximum response observed at pH 8.5
684417
5.5 - 11
-
1 h, stable
655274
5.5 - 9
-
25°C, 24 h, stable, wild-type enzyme and mutant enzyme F42C
672703
6 - 8.2
6 - 8.2
stable from pH 6.0 to 8.2, above which a slight but continuous decrease in protein stability is observed
684631
6 - 8
-
stable
654786
6 - 8.2
6 - 9
-
wild-type enzyme and mutant enzyme DELTASer308-Lys321 are stable betweeen
655558
6 - 8.2
Q1AYM8
stable from pH 6.0 to 8.2, above which a slight but continuous decrease in protein stability is observed
684631
6 - 8
-
stable
654786
6 - 10
-
-
713128
6 - 8
-
stable
654786
6 - 8.2
-
stable from pH 6.0 to 8.2, above which a slight but continuous decrease in protein stability is observed
684631
7.3 - 7.9
-
30 min stable
391833
8 - 9
-
at pH 8 immobilized enzyme (onto Ni2+-chelated NTA magnetic beads) activity decreases to 44% while the free enzyme showed optimal activity, the activities of both enzyme forms decay similarily at pH values above 9, the free enzyme activity is less affected for pH values below 9 than the immobilized enzyme
687855
8
-
unstable above
391852
10.5
-
remains fully stable up to pH 10.5
684631