1.4.3.3: D-amino-acid oxidase

This is an abbreviated version!
For detailed information about D-amino-acid oxidase, go to the full flat file.

Word Map on EC 1.4.3.3

Reaction

a D-amino acid
+
H2O
+
O2
=
a 2-oxo carboxylate
+
NH3
+
H2O2

Synonyms

chDAO, D-AAO, D-amino acid oxidase, D-amino acid oxidases, D-amino-acid-oxidase, D-aminoacid oxidase, DAAO, DAMOX, DAO, DAO1, DaoE, hDAAO, ophio-amino-acid oxidase, oxidase, D-amino acid, PEG-DAO, pkDAAO, RgDAAO, TvDAAO, TvDAO,  LH99

ECTree

     1 Oxidoreductases
         1.4 Acting on the CH-NH2 group of donors
             1.4.3 With oxygen as acceptor
                1.4.3.3 D-amino-acid oxidase

KM Value

KM Value on EC 1.4.3.3 - D-amino-acid oxidase

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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.015
2,6-dichlorophenolindophenol
-
with D-alanine
0.83 - 28
cephalosporin C
6.7
citrulline
-
-
0.03 - 0.04
D-1-naphthyl-alanine
0.05 - 0.33
D-1-naphthyl-glycine
0.46
D-2-aminobutyrate
-
-
0.13 - 0.4
D-2-chloro-phenylglycine
0.5
D-2-fluoro-phenylglycine
-
mutant enzyme M213G, in 75 mM sodium diphosphate buffer, pH 8.5 at 25°C; wild type enzyme, in 75 mM sodium diphosphate buffer, pH 8.5 at 25°C
0.04 - 0.06
D-2-naphthyl-alanine
0.01 - 0.03
D-2-naphthyl-glycine
0.03 - 0.2
D-4-chloro-phenylglycine
0.6 - 0.7
D-4-fluoro-phenylglycine
1.6 - 2.1
D-4-hydroxy-phenylglycine
0.23 - 46
D-Ala
0.036 - 800
D-alanine
1.01 - 13
D-Arg
1.3 - 29
D-arginine
11 - 50
D-Asn
2 - 2000
D-Asp
2 - 2000
D-Aspartate
21.41
D-cysteine
-
-
1.5
D-Dopa
3.8 - 4.6
D-Gln
33 - 77.3
D-Glu
5.1 - 101
D-glutamate
2.5 - 6
D-glutamine
2.89
D-His
-
pH 8.3, 37°C, recombinant DAO
0.15 - 0.3
D-homo-phenylalanine
0.052 - 1.06
D-Ile
0.049 - 6.5
D-Leu
0.08 - 10.9
D-leucine
0.425 - 29.3
D-Lys
8 - 16.6
D-Lysine
0.2 - 4.2
D-Met
0.24 - 143
D-methionine
0.39 - 0.4
D-norleucine
0.063 - 0.89
D-Norvaline
0.04 - 3.6
D-Phe
0.2 - 55.9
D-phenylalanine
0.38 - 7
D-phenylglycine
0.33 - 1280
D-Pro
1.1 - 86
D-proline
0.909 - 36.6
D-Ser
3.9 - 310
D-serine
8.16 - 11.1
D-Thr
0.2 - 2.7
D-Trp
0.17 - 15
D-tryptophan
0.087 - 0.84
D-Tyr
1.1 - 1.5
D-tyrosine
0.094 - 46
D-Val
0.67 - 20
D-valine
1
Ethionine
-
-
0.00045
FAD
-
-
140 - 180
Gly
180
glycine
-
at pH 7.0 and 25°C
43.11 - 362
L-Ala
45
L-Asp
-
at pH 8.0 and 37°C
20 - 1800
L-Pro
0.067
methylene blue
-
with D-alanine
18
N-Methyl-D-aspartate
-
pH 8.5, 25°C, mutant enzyme M213R
0.06 - 6.1
O2
0.06
phenylalanine
-
-
17.3 - 20.8
serine
1.99
thiazolidine-2-carboxylate
-
-
5.8
tyrosine
-
-
additional information
additional information
-