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(1R)-1-(2-naphthyl)ethylamine + H2O + O2
1-(naphthalen-2-yl)ethan-1-one + NH3 + H2O2
substrate of mutant Y228L/F242I/R283G
-
-
?
(1R)-1-phenylethan-1-amine + H2O + O2
1-phenylethan-1-one + NH3 + H2O2
(1R)-1-phenylpropan-1-amine + H2O + O2
1-phenylpropan-1-one + NH3 + H2O2
mutant Y228L/R283G, 109% of the activity with (1R)-1-phenylethan-1-amine
-
-
?
(R)-alpha-methylbenzylamine + H2O + O2
alpha-methylbenzaldehyde + NH3 + H2O2
substrate of mutant Y228L/R283G, the neutral amine is the substrate
-
-
?
(RS)-1-(4-chlorophenyl)-1-phenylmethanamine + H2O + O2
(4-chlorophenyl)(phenyl)methanone + NH3 + H2O2
no substrate of wild-type
-
-
?
1,1-diphenylmethanamine + H2O + O2
diphenylmethanone + NH3 + H2O2
no substrate of wild-type
-
-
?
1-(4-fluorophenyl)ethan-1-amine + H2O + O2
1-phenylethan-1-one + NH3 + H2O2
mutant Y228L/R283G, 22.7% of the activity with (1R)-1-phenylethan-1-amine
-
-
?
3,4-dihydroxy-D-phenylalanine + H2O + O2
3,4-dihydroxyphenylpyruvate + NH3 + H2O2
-
-
-
-
?
4-fluoro-D-Phe + H2O + O2
4-fluorophenylpyruvate + NH3 + H2O2
82% activity compared to D-Met
-
-
?
4-fluoro-DL-phenylalanine + H2O + O2
4-fluoro-phenylpyruvate + NH3 + H2O2
81% activity compared to D-methionine
-
-
?
a D-amino acid + H2O + O2
a 2-oxo acid + NH3 + H2O2
alanine + H2O + O2
pyruvate + NH3 + H2O2
-
-
-
-
?
cephalosporin C
alpha-ketoadipinyl-7-aminocephalosporanic acid
-
-
-
-
?
cephalosporin C + H2O + O2
7-(5-oxoadipoamido)cephalosporanic acid + NH3 + H2O2
cephalosporin C + H2O + O2
7-aminocephalosporanic acid + ? + H2O2
conversion of cephalosporin C to 7-aminocephalosporanic acid and spontaneous decarboxylation of oxoadipyl-7-amino cephalosporanic acid is promoted by the H2O2 formed in the oxidase reaction of TvDAO
-
-
?
D-1-naphthyl-alanine + H2O + O2
?
-
-
-
?
D-1-naphthyl-glycine + H2O + O2
?
-
-
-
?
D-2-aminobutyrate + H2O + O2
2-oxobutyrate + NH3 + H2O2
-
-
-
-
?
D-2-chloro-phenylglycine + H2O + O2
(2-chlorophenyl)acetic acid + NH3 + H2O2
-
-
-
?
D-2-fluoro-phenylglycine + H2O + O2
(2-fluorophenyl)acetic acid + NH3 + H2O2
-
-
-
?
D-2-naphthyl-alanine + H2O + O2
?
-
-
-
?
D-2-naphthyl-glycine + H2O + O2
?
-
-
-
?
D-3,4-dihydroxyphenylalanine + H2O + O2
?
D-3,4-dihydroxyphenylalanine + H2O + O2
? + H2O2 + NH3
D-4-chloro-phenylglycine + H2O + O2
(4-chlorophenyl)acetic acid + NH3 + H2O2
-
-
-
?
D-4-fluoro-phenylglycine + H2O + O2
(4-fluorophenyl)acetic acid + NH3 + H2O2
-
-
-
?
D-4-hydroxy-phenylglycine + H2O + O2
(4-hydroxyphenyl)acetic acid + NH3 + H2O2
-
-
-
?
D-Ala + H2O + O2
pyruvate + NH3 + H2O2
D-alanine + 2,6-dichlorophenolindophenol + H2O
pyruvate + reduced 2,6-dichlorophenolindophenol + H2O2
-
-
-
-
r
D-alanine + H2O + O2
pyruvate + H2O2 + NH3
D-alanine + H2O + O2
pyruvate + NH3 + H2O2
D-alanine + H2O + O2
pyruvic acid + NH3 + H2O2
D-alanine + oxidized methylene blue + H2O
pyruvate + reduced methylene blue + H2O2
-
-
-
-
r
D-allo-isoleucine + H2O + O2
(3R)-3-hydroxy-2-oxobutanoate + NH3 + H2O2
-
-
-
?
D-allo-isoleucine + H2O + O2
?
1.0% activity compared to D-methionine
-
-
?
D-allo-threonine + H2O + O2
? + NH3 + H2O2
-
-
-
?
D-alpha-aminoadipate + H2O + O2
2-oxoadipate + NH3 + H2O2
-
-
-
-
?
D-amino acid + H2O + O2
2-oxo acid + NH3 + H2O2
D-amino acid + H2O + O2
2-oxocarboxylate + NH3 + H2O2
D-amino acid + H2O + O2
a 2-oxo carboxylate + NH3 + H2O2
-
-
-
-
ir
D-Arg + H2O + O2
5-guanidino-2-oxopentanoate + NH3 + H2O2
D-Arg + H2O + O2
5-guanidino-2-oxopentanoic acid + NH3 + H2O2
D-arginine + H2O + O2
5-guanidino-2-oxopentanoate + NH3 + H2O2
D-arginine + H2O + O2
5-guanidino-2-oxopentanoic acid + NH3 + H2O2
D-Asn + H2O + O2
2-oxosuccinamate + NH3 + H2O2
D-Asp + H2O + O2
oxaloacetate + NH3 + H2O2
D-asparagine + H2O + O2
2-oxosuccinamic acid + NH3 + H2O2
D-aspartate + H2O + O2
?
-
-
-
?
D-aspartate + H2O + O2
? + NH3 + H2O2
recombinant maltose-binding protein-fusion DAAO
-
-
?
D-aspartate + H2O + O2
oxaloacetate + NH3 + H2O2
D-aspartic acid + H2O + O2
oxaloacetate + NH3 + H2O2
-
weak activity
-
-
?
D-citrulline + H2O + O2
2-oxo-5-ureidopentanoate + NH3 + H2O2
-
-
-
-
r
D-citrulline + H2O + O2
2-oxo-5-ureidopentanoic acid + NH3 + H2O2
-
-
-
-
?
D-cycloserine + H2O + O2
? + NH3 + H2O2
-
-
-
?
D-Cys + H2O + O2
2-oxo-3-sulfanylpropionate + NH3 + H2O2
-
1% of the activity with D-Met
-
-
?
D-cysteine + H2O + O2
2-oxo-3-sulfanylpropionate + NH3 + H2O2
D-cysteine + H2O + O2
2-oxo-3-thiopropionic acid + NH3 + H2O2
D-DOPA + H2O + O2
3,4-dihydroxyphenylpyruvate + NH3 + H2O2
-
-
-
?
D-DOPA + H2O + O2
3,4-dihydroxyphenylpyruvic acid + NH3 + H2O2
D-ethionine + H2O + O2
4-ethylsulfanyl-2-oxobutyric acid + NH3 + H2O2
D-Gln + H2O + O2
2-oxoglutaramate + NH3 + H2O2
D-Glu + H2O + O2
alpha-ketoglutarate + NH3 + H2O2
D-glufosinate + H2O + O2
?
-
-
-
-
ir
D-glutamate + H2O + O2
2-oxoglutarate + NH3 + H2O2
D-glutamate + H2O + O2
alpha-ketoglutarate + NH3 + H2O2
D-glutamic acid + H2O + O2
alpha-ketoglutarate + NH3 + H2O2
-
-
-
-
?
D-glutamine + H2O + O2
2-oxoglutaramate + NH3 + H2O2
D-His + H2O + O2
3-(1H-imidazol-4-yl)-2-oxopropanoic acid + NH3 + H2O2
D-histidine + H2O + O2
3-(1H-imidazol-4-yl)-2-oxopropanoate + NH3 + H2O2
D-histidine + H2O + O2
3-(1H-imidazol-4-yl)-2-oxopropanoic acid + NH3 + H2O2
-
-
-
?
D-homo-phenylalanine + H2O + O2
?
-
-
-
?
D-Ile + H2O + O2
3-methyl-2-oxopentanoate + NH3 + H2O2
D-isoleucine + H2O + O2
3-methyl-2-oxopentanoate + NH3 + H2O2
D-isoleucine + H2O + O2
3-methyl-2-oxopentanoic acid + NH3 + H2O2
D-kynurenine + H2O + O2
kynurenate + NH3 + H2O2
-
-
-
?
D-kynurenine + H2O + O2
kynurenic acid + NH3 + H2O2
-
-
-
-
?
D-kynurenine + NH3 + H2O2
kynurenic acid + NH3 + H2O2
-
-
-
-
?
D-Leu + H2O + O2
4-methyl-2-oxopentanoate + NH3 + H2O2
D-Leu + H2O + O2
4-methyl-2-oxopentanoic acid + NH3 + H2O2
-
-
-
-
?
D-leucine + H2O + O2
4-methyl-2-oxopentanoate + NH3 + H2O2
-
-
-
?
D-leucine + H2O + O2
4-methyl-2-oxopentanoic acid + NH3 + H2O2
D-Lys + H2O + O2
6-amino-2-oxohexanoic acid + NH3 + H2O2
D-Lys + H2O + O2
? + NH3 + H2O2
-
-
-
?
D-lysine + H2O + O2
6-amino-2-oxohexanoate + NH3 + H2O2
-
-
-
?
D-lysine + H2O + O2
6-amino-2-oxohexanoic acid + NH3 + H2O2
D-Met + H2O + O2
4-methylthio-2-oxobutanoate + NH3 + H2O2
D-Met + H2O + O2
4-methylthio-2-oxobutanoic acid + NH3 + H2O2
-
best substrate
-
-
?
D-methionine + 2,6-dichloroindophenol
4-methylsulfanyl-2-oxobutanoate + reduced 2,6-dichloroindophenol
D-methionine + 2,6-dichlorophenolindophenol
?
-
-
-
-
?
D-methionine + 2,6-dichlorophenolindophenol + H2O
?
-
-
-
-
?
D-methionine + H2O + 2,6-dichloroindophenol
?
-
-
-
-
?
D-methionine + H2O + O2
2-oxo-4-methylthiobutyric acid + NH3 + H2O2
-
-
-
-
ir
D-methionine + H2O + O2
4-methylsulfanyl-2-oxobutanoate + NH3 + H2O2
D-methionine + H2O + O2
4-methylthio-2-oxobutanoate + NH3 + H2O2
-
highly active
-
-
?
D-methionine + H2O + O2
4-methylthio-2-oxobutanoic acid + NH3 + H2O2
D-naphthylalanine + H2O + O2
naphthylpyruvate + NH3 + H2O2
-
-
-
?
D-norleucine + H2O + O2
2-oxohexanoate + NH3 + H2O2
D-norvaline + H2O + O2
2-oxopentanoate + NH3 + H2O2
D-Orn + H2O + O2
5-amino-2-oxopentanoate + NH3 + H2O2
34% activity compared to D-Met
-
-
?
D-ornithine + H2O + O2
5-amino-2-oxopentanoate + NH3 + H2O2
D-Phe + H2O + O2
phenylpyruvate + NH3 + H2O2
D-phenylalanine + H2O + O2
?
-
-
-
?
D-phenylalanine + H2O + O2
phenylpyruvate + NH3 + H2O2
D-phenylalanine + H2O + O2
phenylpyruvic acid + NH3 + H2O2
D-phenylglycine + H2O + O2
benzoylformic acid + NH3 + H2O2
D-Pro + H2O + O2
2-oxopentanoate + NH3 + H2O2
D-Pro + H2O + O2
2-oxopentanoic acid + NH3 + H2O2
D-proline + H2O + O2
2-oxopentanoate + NH3 + H2O2
D-proline + H2O + O2
2-oxopentanoic acid + NH3 + H2O2
D-Ser + H2O + O2
2-oxo-3-hydroxypropionate + NH3 + H2O2
D-Ser + H2O + O2
2-oxo-3-hydroxypropionic acid + NH3 + H2O2
-
-
-
-
?
D-serine + H2O + O2
2-oxo-3-hydroxypropanoate + NH3 + H2O2
-
-
-
?
D-serine + H2O + O2
2-oxo-3-hydroxypropionate + NH3 + H2O2
D-serine + H2O + O2
2-oxo-3-hydroxypropionic acid + NH3 + H2O2
D-serine + H2O + O2
3-hydroxypyruvate + NH3 + H2O2
D-serine + H2O + O2
?
-
-
-
?
D-serine + H2O + O2
? + NH3 + H2O2
1.4.3.3 D-amino-acid oxidase
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