1.4.3.3: D-amino-acid oxidase

This is an abbreviated version!
For detailed information about D-amino-acid oxidase, go to the full flat file.

Word Map on EC 1.4.3.3

Reaction

a D-amino acid
+
H2O
+
O2
=
a 2-oxo carboxylate
+
NH3
+
H2O2

Synonyms

chDAO, D-AAO, D-amino acid oxidase, D-amino acid oxidases, D-amino-acid-oxidase, D-aminoacid oxidase, DAAO, DAMOX, DAO, DAO1, DaoE, hDAAO, ophio-amino-acid oxidase, oxidase, D-amino acid, PEG-DAO, pkDAAO, RgDAAO, TvDAAO, TvDAO, LH99

ECTree

     1 Oxidoreductases
         1.4 Acting on the CH-NH2 group of donors
             1.4.3 With oxygen as acceptor
                1.4.3.3 D-amino-acid oxidase

Renatured

Renatured on EC 1.4.3.3 - D-amino-acid oxidase

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RENATURED/Commentary
ORGANISM
UNIPROT
LITERATURE
holo- and apoprotein samples of hDAAO are incubated for 60 min at 15C in a buffer containing different concentrations of urea, 0-6 M, and then refolded by 10fold dilution in 50 mM sodium diphosphate, pH 8.0, 5% glycerol and 15C, in the presence of a 10fold molar excess of FAD. Chemical denaturation of hDAAO holoenzyme is partially reversible, 50% of the initial activity is recovered starting with the refolding from 4 M urea-denatured holoprotein, while the refolding of apoprotein is largely irreversible even at 2 M urea , 15-20% of recovery of enzymatic activity versus 90-100% for the holoenzyme and even in the presence of the cofactor
-
renaturation studies of free and immobilized enzyme
-