1.4.3.3: D-amino-acid oxidase

This is an abbreviated version!
For detailed information about D-amino-acid oxidase, go to the full flat file.

Word Map on EC 1.4.3.3

Reaction

a D-amino acid
+
H2O
+
O2
=
a 2-oxo carboxylate
+
NH3
+
H2O2

Synonyms

chDAO, D-AAO, D-amino acid oxidase, D-amino acid oxidases, D-amino-acid-oxidase, D-aminoacid oxidase, DAAO, DAMOX, DAO, DAO1, DaoE, hDAAO, ophio-amino-acid oxidase, oxidase, D-amino acid, PEG-DAO, pkDAAO, RgDAAO, TvDAAO, TvDAO, LH99

ECTree

     1 Oxidoreductases
         1.4 Acting on the CH-NH2 group of donors
             1.4.3 With oxygen as acceptor
                1.4.3.3 D-amino-acid oxidase

Storage Stability

Storage Stability on EC 1.4.3.3 - D-amino-acid oxidase

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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20C, immobilized on microbiosensor, more than 160 days, 2% loss of activity
-20C, several months
-
-20C, wild-type and mutant enzyme M213R are stable for several months
-
-80C, 2-3 months, stable
-
22C, free enzyme, 10 days, 20% residual activity
-
22C, immobilized enzyme, 10 days, 74% residual activity
-
25C, free enzyme, 14 days, 50% loss of activity
-
25C, immobilized enzyme, 18 days, 50% loss of activity
-
30C, 1 week, no loss of activity
4C, immobilized enzyme, 1 month, 10% loss of activity
4C, pH 6.5, 3.2 M ammonium sulfate, crystallized, several months
-
frozen, 50% loss of activity after 7 days
-