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Literature summary for 1.4.3.3 extracted from

  • Sacchi, S.; Caldinelli, L.; Cappelletti, P.; Pollegioni, L.; Molla, G.
    Structure-function relationships in human D-amino acid oxidase (2012), Amino Acids, 43, 1833-1850.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
3-hydroxyquinoline-2-(1H)-one very potent inhibitor Homo sapiens
4H-furo[3,2-b]pyrrole-5-carboxylic acid
-
Homo sapiens
4H-thieno[3,2-b]pyrrole-5-carboxylic acid
-
Homo sapiens
5-methylpyrazole-3-carboxylic acid
-
Homo sapiens
6-chlorobenzo[d]isoxazol-3-ol
-
Homo sapiens
anthranilate
-
Homo sapiens
benzoate competitive inhibitor Homo sapiens
chlorpromazine
-
Homo sapiens
D-serine
-
Homo sapiens
trifluoro-D-alanine pseudo-substrate Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3
-
D-alanine at pH 7.0 and 25°C Homo sapiens
1.5
-
D-tryptophan at pH 7.0 and 25°C Homo sapiens
1.5
-
D-Dopa at pH 7.0 and 25°C Homo sapiens
1.5
-
D-tyrosine at pH 7.0 and 25°C Homo sapiens
2.7
-
D-phenylalanine at pH 7.0 and 25°C Homo sapiens
7.5
-
D-serine at pH 7.0 and 25°C Homo sapiens
8.5
-
D-proline at pH 7.0 and 25°C Homo sapiens
180
-
glycine at pH 7.0 and 25°C Homo sapiens
2000
-
D-Aspartate at pH 7.0 and 25°C Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
peroxisome
-
Homo sapiens 5777
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-amino acid + H2O + O2 Homo sapiens
-
2-oxocarboxylate + NH3 + H2O2
-
?
D-kynurenine + H2O + O2 Homo sapiens
-
kynurenic acid + NH3 + H2O2
-
?
D-serine + H2O + O2 Homo sapiens low catalytic efficiency and substrate affinity on the physiological substrate D-serine 2-oxo-3-hydroxypropionate + NH3 + H2O2
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
Homo sapiens
-
brain cortex
-
Homo sapiens
-
cerebellum
-
Homo sapiens
-
glial cell
-
Homo sapiens
-
medulla oblongata
-
Homo sapiens
-
additional information not detected in the forebrain Homo sapiens
-
pons
-
Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-alanine + H2O + O2
-
Homo sapiens pyruvate + NH3 + H2O2
-
?
D-amino acid + H2O + O2
-
Homo sapiens 2-oxocarboxylate + NH3 + H2O2
-
?
D-aspartate + H2O + O2
-
Homo sapiens oxaloacetate + NH3 + H2O2
-
?
D-DOPA + H2O + O2
-
Homo sapiens 3,4-dihydroxyphenylpyruvic acid + NH3 + H2O2
-
?
D-kynurenine + H2O + O2
-
Homo sapiens kynurenic acid + NH3 + H2O2
-
?
D-phenylalanine + H2O + O2
-
Homo sapiens phenylpyruvate + NH3 + H2O2
-
?
D-proline + H2O + O2
-
Homo sapiens 2-oxopentanoic acid + NH3 + H2O2
-
?
D-serine + H2O + O2 low catalytic efficiency and substrate affinity on the physiological substrate D-serine Homo sapiens 2-oxo-3-hydroxypropionate + NH3 + H2O2
-
?
D-tryptophan + H2O + O2
-
Homo sapiens indol-3-pyruvic acid + NH3 + H2O2
-
?
D-tyrosine + H2O + O2
-
Homo sapiens 4-hydroxyphenylpyruvic acid + NH3 + H2O2
-
?
glycine + H2O + O2
-
Homo sapiens glyoxylate + NH3 + H2O2
-
?

Subunits

Subunits Comment Organism
homodimer
-
Homo sapiens

Synonyms

Synonyms Comment Organism
D-amino acid oxidase
-
Homo sapiens
DAAO
-
Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.9
-
glycine at pH 7.0 and 25°C Homo sapiens
3
-
D-serine at pH 7.0 and 25°C Homo sapiens
3.2
-
D-tryptophan at pH 7.0 and 25°C Homo sapiens
5.2
-
D-alanine at pH 7.0 and 25°C Homo sapiens
6.6
-
D-phenylalanine at pH 7.0 and 25°C Homo sapiens
6.7
-
D-Aspartate at pH 7.0 and 25°C Homo sapiens
10.2
-
D-proline at pH 7.0 and 25°C Homo sapiens
14.8
-
D-tyrosine at pH 7.0 and 25°C Homo sapiens
21.7
-
D-Dopa at pH 7.0 and 25°C Homo sapiens

Cofactor

Cofactor Comment Organism Structure
FAD
-
Homo sapiens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.007
-
benzoate at pH 7.0 and 25°C Homo sapiens
0.7
-
chlorpromazine at pH 7.0 and 25°C Homo sapiens
0.7
-
trifluoro-D-alanine at pH 7.0 and 25°C Homo sapiens

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.000004
-
at pH 7.0 and 25°C Homo sapiens 3-hydroxyquinoline-2-(1H)-one
0.000141
-
at pH 7.0 and 25°C Homo sapiens 4H-furo[3,2-b]pyrrole-5-carboxylic acid
0.000145
-
at pH 7.0 and 25°C Homo sapiens 4H-thieno[3,2-b]pyrrole-5-carboxylic acid
0.00018
-
at pH 7.0 and 25°C Homo sapiens 6-chlorobenzo[d]isoxazol-3-ol
0.00091
-
at pH 7.0 and 25°C Homo sapiens 5-methylpyrazole-3-carboxylic acid

General Information

General Information Comment Organism
malfunction a total loss of enzymatic activity is associated with the adult onset of familial amyotrophic lateral sclerosis. Altered enzyme expression levels and activity have been reported in schizophrenia Homo sapiens
physiological function the enzyme is part responsible for the significant increases in brain kynurenic acid levels seen under inflammatory conditions Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.003
-
D-Aspartate at pH 7.0 and 25°C Homo sapiens
0.005
-
glycine at pH 7.0 and 25°C Homo sapiens
0.4
-
D-serine at pH 7.0 and 25°C Homo sapiens
1.3
-
D-proline at pH 7.0 and 25°C Homo sapiens
2.1
-
D-tryptophan at pH 7.0 and 25°C Homo sapiens
2.4
-
D-phenylalanine at pH 7.0 and 25°C Homo sapiens
4
-
D-alanine at pH 7.0 and 25°C Homo sapiens
9.9
-
D-tyrosine at pH 7.0 and 25°C Homo sapiens
14.5
-
D-Dopa at pH 7.0 and 25°C Homo sapiens