1.11.1.13: manganese peroxidase

This is an abbreviated version!
For detailed information about manganese peroxidase, go to the full flat file.

Word Map on EC 1.11.1.13

Reaction

2 Mn(II) + 2 H+ +

H2O2
= 2 Mn(III) + 2 H2O

Synonyms

hybrid Mn-peroxidase, L-MnP, LeMnP2, manganese peroxidase, manganese-dependent peroxidase, Mn-dependent (NADH-oxidizing) peroxidase, Mn2+: hydrogen peroxide oxidoreductase, Mn2+:hydrogen peroxide oxidoreductase, MnP, MnP 1, MnP II, MnP-GY, MnP-PGY, mnp1, MnP2, MnP3, MnP6, MP, multifunctional manganese peroxidase, Nf b19 MNP2, peroxidase, manganese, peroxidase-M2

ECTree

     1 Oxidoreductases
         1.11 Acting on a peroxide as acceptor
             1.11.1 Peroxidases
                1.11.1.13 manganese peroxidase

Specific Activity

Specific Activity on EC 1.11.1.13 - manganese peroxidase

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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.01
-
specific MnP activity recovered from solid-state culture after 1h and 10°C, U/mg of fugal biomass, pH 6.0; specific MnP activity recovered from solid-state culture after 1h and 25°C, U/mg of fugal biomass, pH 6.0
0.04
-
effect of dialysis on MnP recovered from solid-state culture at 10°C, dialyzed extract, extraction conducted for 3h at 100 rpm, U/mg of fugal biomass, pH 4.0
0.1
-
specific MnP activity recovered from solid-state culture after 3h and 10°C, U/mg of fugal biomass, pH 6.0
0.12
-
specific MnP activity recovered from solid-state culture after 3h and 25°C, U/mg of fugal biomass, pH 6.0
0.16
-
effect of dialysis on MnP recovered from solid-state culture at 10°C, dialyzed extract, extraction conducted for 1h without agitation, U/mg of fugal biomass, pH 5.0
0.2
-
specific MnP activity recovered from solid-state culture after 1h and 25°C, U/mg of fugal biomass, pH 4.0
0.21
-
effect of dialysis on MnP recovered from solid-state culture at 10°C, dialyzed extract, extraction conducted for 3h at 100 rpm, U/mg of fugal biomass, pH 5.0
0.27
-
effect of dialysis on MnP recovered from solid-state culture at 10°C, dialyzed extract, extraction conducted for 1h without agitation, U/mg of fugal biomass, pH 4.0
0.31
-
effect of dialysis on MnP recovered from solid-state culture at 10°C, crude extract, extraction conducted for 3h at 100 rpm, U/mg of fugal biomass, pH 4.0; specific MnP activity recovered from solid-state culture after 3h and 10°C, U/mg of fugal biomass, pH 4.0
0.35
-
effect of dialysis on MnP recovered from solid-state culture at 10°C, crude extract, extraction conducted for 1h without agitation, U/mg of fugal biomass, pH 4.0; effect of dialysis on MnP recovered from solid-state culture at 10°C, crude extract, extraction conducted for 1h without agitation, U/mg of fugal biomass, pH 5.0; specific MnP activity recovered from solid-state culture after 1h and 10°C, U/mg of fugal biomass, pH 4.0; specific MnP activity recovered from solid-state culture after 1h and 10°C, U/mg of fugal biomass, pH 5.0
0.41
-
effect of dialysis on MnP recovered from solid-state culture at 10°C, crude extract, extraction conducted for 3h at 100 rpm, U/mg of fugal biomass, pH 5.0; specific MnP activity recovered from solid-state culture after 3h and 10°C, U/mg of fugal biomass, pH 5.0
0.45
-
specific MnP activity recovered from solid-state culture after 1h and 25°C, U/mg of fugal biomass, pH 5.0
0.48
-
specific MnP activity recovered from solid-state culture after 3h and 25°C, U/mg of fugal biomass, pH 4.0
0.7
-
specific MnP activity recovered from solid-state culture after 3h and 25°C, U/mg of fugal biomass, pH 5.0
10.2
-
oxidation of 2,2-azino-di-3-ethylbenzothiazoline-6-sulfonate in presence of Mn2+ and H2O2
17.2
-
recombinant protein, pH 4.5, 37°C
40
-
culture supernatant, pH 5.5, 45°C; purification step culture supernatant
41
-
purification step ultrafiltration
65 - 134
-
depending on culture age
107
-
isoenzyme H4
169
-
purification step chromatography on a concanavalin-A Sepharose column
180
-
Mn3+-lactate complex formation
185
-
purification step crude medium
288
-
after 7.2fold purification, pH 5.5, 45°C; purification step gel filtration
506
-
pH 5.0, 25°C
660
-
o-toluidine as substrate for Mn3+-oxidation
733
-
purification step DEAE Sepharose
870
-
purification step Mono Q1
1230
-
purification step CIM QA disk 3, chromatographic MnP form 3, CIM3
2250
-
purification step Mono Q2
2480
-
purification step CIM QA disk 1, chromatographic MnP form 1, CIM1
3430
-
purification step CIM QA disk 2, chromatographic MnP form 2, CIM2
additional information