Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.11.1.13 extracted from

  • Urek, R.O.; Pazarlioglu, N.K.
    Purification and partial characterization of manganese peroxidase from immobilized Phanerochaete chrysosporium (2004), Proc. Biochem., 39, 2061-2068.
No PubMed abstract available
Search for more literature for 1.11.1.13

General Stability

General Stability Organism
complete loss of activity after 1 h in 2 mM KCN Phanerodontia chrysosporium
no inactivation after 5 h in 50 mM Na-tartrate buffer, pH 4.5, with 2 mM nitrilotriacetate, 0.5 mM iodoacetamide or 2 mM PMSF Phanerodontia chrysosporium

Inhibitors

Inhibitors Comment Organism Structure
acetate 1 mM, 58% inhibition Phanerodontia chrysosporium
ascorbic acid 2 mM, complete inhibition Phanerodontia chrysosporium
beta-mercaptoethanol 2 mM, complete inhibition Phanerodontia chrysosporium
N-bromosuccinimide
-
 Phanerodontia chrysosporium
N-ethyl-5-phenylisoxazolium 3'-sulfonate 1 mM, 28% inhibition Phanerodontia chrysosporium
NaN3 2 mM, complete inhibition Phanerodontia chrysosporium

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0286
-
 2,6-dimethoxyphenol 30°C, pH 4.5 Phanerodontia chrysosporium
0.0714
-
 H2O2 30°C, pH 4.5 Phanerodontia chrysosporium

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ the proposed role of Mn2+ chelators in the enzyme mechanism is to release Mn3+ from the enzyme Phanerodontia chrysosporium

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45000
-
 gel filtration Phanerodontia chrysosporium

Organism

Organism UniProt Comment Textmining
Phanerodontia chrysosporium
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Phanerodontia chrysosporium

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
484.8
-
-
 Phanerodontia chrysosporium

Storage Stability

Storage Stability Organism
-20°C, 10% decrease of activity after 3 months Phanerodontia chrysosporium
4°C, pH 4.5, purified enzyme in 50 mM Na-tartrate buffer is stable for 1 week Phanerodontia chrysosporium

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,6-dimethoxyphenol + H2O2
-
 Phanerodontia chrysosporium ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 45000, SDS-PAGE Phanerodontia chrysosporium

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
 Phanerodontia chrysosporium

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
25 45 25°C: about 80% of maximal activity, 45°C: about 60% of maximal activity Phanerodontia chrysosporium

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
35
-
 pH 4.5, 1 h, stable up to Phanerodontia chrysosporium
45
-
 pH 4.5, 1 h, about 40% loss of activity Phanerodontia chrysosporium
47
-
 pH 4.5, 1 h, about 60% loss of activity Phanerodontia chrysosporium
65
-
 pH 4.5, 1 h, complete loss of activity Phanerodontia chrysosporium

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5
-
-
 Phanerodontia chrysosporium

pH Range

pH Minimum pH Maximum Comment Organism
3.5 6 pH 3.5: about 30% of maximal activity, pH 4.0: about 70% of maximal activity, pH 6.0: about 45% of maximal activity Phanerodontia chrysosporium

pH Stability

pH Stability pH Stability Maximum Comment Organism
2.5
-
 25°C, 2 h, about 50% loss of activity Phanerodontia chrysosporium
3
-
 25°C, 2 h, about 40% loss of activity Phanerodontia chrysosporium
4.5 6 25°C, 2 h, stable Phanerodontia chrysosporium
6.5
-
 25°C, 2 h, about 20% loss of activity Phanerodontia chrysosporium
7
-
 25°C, 2 h, about 40% loss of activity Phanerodontia chrysosporium