1.11.1.13: manganese peroxidase

This is an abbreviated version!
For detailed information about manganese peroxidase, go to the full flat file.

Word Map on EC 1.11.1.13

Reaction

2 Mn(II) + 2 H+ +

H2O2
= 2 Mn(III) + 2 H2O

Synonyms

hybrid Mn-peroxidase, L-MnP, LeMnP2, manganese peroxidase, manganese-dependent peroxidase, Mn-dependent (NADH-oxidizing) peroxidase, Mn2+: hydrogen peroxide oxidoreductase, Mn2+:hydrogen peroxide oxidoreductase, MnP, MnP 1, MnP II, MnP-GY, MnP-PGY, mnp1, MnP2, MnP3, MnP6, MP, multifunctional manganese peroxidase, Nf b19 MNP2, peroxidase, manganese, peroxidase-M2

ECTree

     1 Oxidoreductases
         1.11 Acting on a peroxide as acceptor
             1.11.1 Peroxidases
                1.11.1.13 manganese peroxidase

Cloned

Cloned on EC 1.11.1.13 - manganese peroxidase

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CLONED/commentary
ORGANISM
UNIPROT
LITERATURE
cDNA encoding MnP2 is cloned and expressed in Escherichia coli BL21(DE3)LysS
cDNA sequence encoding a MnP isoenzyme is determined
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cDNA sequences of several MnP-encoding genes, including mnp1, mnp2 and mnp3
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cloning and sequencing of the gene encoding MnP2, promoter region is analyzed, nucleotide sequence of the cDNA encoding MnP1, mnp1 is located on chromosome IV, mnp2 on chromosome VI and mnp3 on chromosome V
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cloning of a full MnP cDNA from Trametes versicolor, and introducing an extra copy of the MnP gene under the control of a glyceraldehyde-3-phosphate dehydrogenase gene promoter from Aspergillus nidulans, into the genome of the wild type strain using a genetic transformation procedure. The gene is cloned and transferred into an expression vector (pBARGPE1) carrying a phosphinothricin resistance gene (bar) as a selectable marker to yield the expression vector, pBARTvMnP2. Transformants are generated through genetic transformation using pBARTvMnP2
expression in Escherichia coli
expression in Pichia pastoris
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expression in Pichia pastoris alphaMnP-1. Production of recombinant manganese peroxidase is highest at pH 6, with rMnP concentrations in the medium declining rapidly at pH less than 5.5, although cell growth rates are similar from pH 4-7
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expression of A48C/A63C double mutant mnp gene in Escherichia coli XL-1 Blue
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expression of mnp2 gene encoding MnP in Phanerochaete chrysosporium
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into the vector pET-22b, used for in vitro transcription and translation in a wheat germ extract system
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isoenzymes are encoded by different genes
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isoenzymes H3, H4 and H5 are encoded by different genes and differentially regulated
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isoenzymes MnPI, MnPII and MnPIII are encoded by separate genes
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isolation of the cDNAs encoding MnP isoenzymes: coding sequences of IZ-MnP1 cDNA with 1152 bp and IZ-MnP2 cDNA with 1155 bp in length, 2 copies of DNA encoding MnP isoenzymes exist in genomic DNA, 4 isoenzymes are generated as products of 2 genes by the difference of posttranslational modification
Deuteromycotina sp.
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lemnp2 is cloned by 3'-RACE using cDNA that is synthesized from mycelial RNA
B5U990
MnP1 and MnP2 are encoded by different genes
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mutant genes F190Y, F190L, F190I and F190A are subcloned and expressed in Escherichia coli XL-1 Blue and DH5alphaF’
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three differentexpression vectors are constructed: pZBMNP contains the native Phanerochaete chrysosporium fungal secretion signal, palphaAMNP contains an alpha-factor secretion signal derived from Saccharomyces cerevisiae, and pZBIMNP has no secretion signal and is used for intracellular expression
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