Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

1.11.1.13: manganese peroxidase

This is an abbreviated version!
For detailed information about manganese peroxidase, go to the full flat file.

Word Map on EC 1.11.1.13

Reaction

2 Mn(II) + 2 H+ +

H2O2
= 2 Mn(III) + 2 H2O

Synonyms

CmMnP, extralong PMnP, hybrid Mn-peroxidase, Il-MnP1, Il-MnP6, L-MnP, LeMnP2, long PMnP, manganese peroxidase, manganese peroxidase 2, manganese-dependent peroxidase, MGmn2, MGmnp1, MGmnp3, Mn-dependent (NADH-oxidizing) peroxidase, Mn2+: hydrogen peroxide oxidoreductase, Mn2+:H2O2 oxidoreductase, Mn2+:hydrogen peroxide oxidoreductase, MnP, MnP 1, MnP II, MnP-BBP6, MnP-GY, MnP-PGY, mnp1, MnP10, MnP117436, MnP12, MnP157986, MnP2, MnP3, MnP50297, MnP6, Moror_3885, MP, MrMnP1, multifunctional manganese peroxidase, Nf b19 MNP2, peroxidase, manganese, peroxidase-M2, rMnP3-BBP6, short manganese peroxidase, short MnP

ECTree

     1 Oxidoreductases
         1.11 Acting on a peroxide as acceptor
             1.11.1 Peroxidases
                1.11.1.13 manganese peroxidase

Cloned

Cloned on EC 1.11.1.13 - manganese peroxidase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
cDNA encoding MnP2 is cloned and expressed in Escherichia coli BL21(DE3)LysS
cDNA sequence encoding a MnP isoenzyme is determined
-
cDNA sequences of several MnP-encoding genes, including mnp1, mnp2 and mnp3
-
cloning and sequencing of the gene encoding MnP2, promoter region is analyzed, nucleotide sequence of the cDNA encoding MnP1, mnp1 is located on chromosome IV, mnp2 on chromosome VI and mnp3 on chromosome V
-
cloning of a full MnP cDNA from Trametes versicolor, and introducing an extra copy of the MnP gene under the control of a glyceraldehyde-3-phosphate dehydrogenase gene promoter from Aspergillus nidulans, into the genome of the wild type strain using a genetic transformation procedure. The gene is cloned and transferred into an expression vector (pBARGPE1) carrying a phosphinothricin resistance gene (bar) as a selectable marker to yield the expression vector, pBARTvMnP2. Transformants are generated through genetic transformation using pBARTvMnP2
codon-optimized gene mrmnp1, high level recombinant expression of wild-type and mutant enzymes in Pichia pastoris strain X-33 with proper heme incorporation in to the resulting enzyme proteins
expression in Escherichia coli
expression in Pichia pastoris
-
expression in Pichia pastoris alphaMnP-1. Production of recombinant manganese peroxidase is highest at pH 6, with rMnP concentrations in the medium declining rapidly at pH less than 5.5, although cell growth rates are similar from pH 4-7
-
expression of A48C/A63C double mutant mnp gene in Escherichia coli XL-1 Blue
-
expression of mnp2 gene encoding MnP in Phanerochaete chrysosporium
-
gene Il-MnP6, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression of His6-tagged enzyme in Escherichia coli strain Rosetta (DE3) in inclusion bodies
gene ilmnp1, recombinant expression of isozyme Il-MnP1
gene MGmnp1, DNA and amino acid sequence determination and analysis, recombinant expression of extracellular isozyme MGmnp1 in Phlebia sp. strain MG-60
gene MGmnp2, DNA and amino acid sequence determination and analysis
gene MGmnp3, DNA and amino acid sequence determination and analysis, recombinant expression of extracellular isozyme MGmnp3 in Phlebia sp. strain MG-60
gene MnP10, DNA and amino acid sequence determination and analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) as soluble protein. Coexpression with disulfide bond isomerase C (DsbC)
gene MnP12, DNA and amino acid sequence determination and analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) as soluble protein. Coexpression with disulfide bond isomerase C (DsbC)
gene MnP2, DNA and amino acid sequence determination and analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) as soluble protein. Coexpression with disulfide bond isomerase C (DsbC)
gene mnp3, recombinant expression of isozyme MnP3 as soluble extracellular protein in Pichia pastoris using alpha-factor as the signal peptide the recombinant enzyme has a MnP activity of 154.5 U/l
into the vector pET-22b, used for in vitro transcription and translation in a wheat germ extract system
isoenzymes are encoded by different genes
-
isoenzymes H3, H4 and H5 are encoded by different genes and differentially regulated
-
isoenzymes MnPI, MnPII and MnPIII are encoded by separate genes
-
isolation of the cDNAs encoding MnP isoenzymes: coding sequences of IZ-MnP1 cDNA with 1152 bp and IZ-MnP2 cDNA with 1155 bp in length, 2 copies of DNA encoding MnP isoenzymes exist in genomic DNA, 4 isoenzymes are generated as products of 2 genes by the difference of posttranslational modification
Deuteromycotina sp.
-
lemnp2 is cloned by 3'-RACE using cDNA that is synthesized from mycelial RNA
MnP1 and MnP2 are encoded by different genes
-
mutant genes F190Y, F190L, F190I and F190A are subcloned and expressed in Escherichia coli XL-1 Blue and DH5alphaF’
-
three differentexpression vectors are constructed: pZBMNP contains the native Phanerochaete chrysosporium fungal secretion signal, palphaAMNP contains an alpha-factor secretion signal derived from Saccharomyces cerevisiae, and pZBIMNP has no secretion signal and is used for intracellular expression
-