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87.1
2,2'-azino-bis(3-ethyl-benzothiazoline-6-sulfonic acid)
-
30°C, pH 4.5
20.2 - 245
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
1.4
2,2'-azino-bis(3-ethylbenzthiazole-6-sulfonic acid)
mutant S168W, 25°C, pH 5.0
1.87 - 20.2
2,2'-azinobis(3-ethylbenzthiazoline)-6-sulfonic acid
1.87
2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid) Mn2+
-
-
120 - 139
2,2-azino-di-3-ethylbenzothiazoline-6-sulfonate
0.4 - 2590
2,6-dimethoxyphenol
98.3
Mn(III)-tartrate
-
preparation M2
-
118
Mn3+-tartrate
-
preparation M1
0.06 - 16.7
Reactive Black 5
7.3
Reactive Blue 19
pH 3.0, 35°C, recombinant His6-tagged enzyme
0.3 - 39.5
veratryl alcohol
additional information
additional information
-
-
-
20.2
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
-
-
245
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
pH 3.5, 45°C, recombinant His6-tagged enzyme
1.87
2,2'-azinobis(3-ethylbenzthiazoline)-6-sulfonic acid
-
in 0.05 M malonate-NaOH buffer pH 4.0 containing 0.2 mM H2O2, 45°C
20.2
2,2'-azinobis(3-ethylbenzthiazoline)-6-sulfonic acid
-
in 0.05 M malonate-NaOH buffer pH 4.0 containing 0.2 mM H2O2 and 1.5 mM Mn2+, 45°C
120
2,2-azino-di-3-ethylbenzothiazoline-6-sulfonate
-
Mn2+-dependent activity
139
2,2-azino-di-3-ethylbenzothiazoline-6-sulfonate
-
Mn2+-independent activity
0.4
2,6-dimethoxyphenol
pH 7.0, 30°C, mutant A172W/A269R
0.5
2,6-dimethoxyphenol
pH 7.0, 30°C, mutant A172W
0.7
2,6-dimethoxyphenol
pH 7.0, 30°C, mutant A172W/A273T
0.8
2,6-dimethoxyphenol
pH 7.0, 30°C, mutant A172W/F259M
1
2,6-dimethoxyphenol
pH 7.0, 30°C, mutant A172W/K168V
1.1
2,6-dimethoxyphenol
pH 7.0, 30°C, mutant A172W/I171V
1.82
2,6-dimethoxyphenol
pH 7.4, 30°C, recombinant wild-type enzyme, in absence of Mn2+
3.98
2,6-dimethoxyphenol
-
-
5.67
2,6-dimethoxyphenol
-
-
5.67
2,6-dimethoxyphenol
-
in 0.05 M malonate-NaOH buffer pH 4.0 containing 0.2 mM H2O2, 45°C
26.26
2,6-dimethoxyphenol
-
-
26.26
2,6-dimethoxyphenol
-
in 0.05 M malonate-NaOH buffer pH 4.0 containing 0.2 mM H2O2 and 1.5 mM Mn2+, 45°C
37.86
2,6-dimethoxyphenol
pH 4.0, 30°C, recombinant wild-type enzyme, in absence of Mn2+
69
2,6-dimethoxyphenol
-
30°C, pH 4.5
70
2,6-dimethoxyphenol
-
Mn2+-independent activity
80.1
2,6-dimethoxyphenol
pH 7.4, 30°C, recombinant wild-type enzyme, in presence of Mn2+
148
2,6-dimethoxyphenol
pH 4.0, 30°C, recombinant wild-type enzyme, in presence of Mn2+
200
2,6-dimethoxyphenol
-
MnP3
320
2,6-dimethoxyphenol
-
Mn2+-dependent activity
379
2,6-dimethoxyphenol
pH 4.0, 30°C, recombinant mutant E166D, in absence of Mn2+
647
2,6-dimethoxyphenol
pH 4.0, 30°C, recombinant mutant E166Q, in absence of Mn2+
989
2,6-dimethoxyphenol
pH 4.5, 55°C, recombinant His6-tagged enzyme
2590
2,6-dimethoxyphenol
pH 4.0, 30°C, recombinant mutant E166G, in absence of Mn2+
3.4 - 4.2
ferrocyanide
-
MnP1 from mutants F190Y, F190L, F190I
4
ferrocyanide
-
wild-type MnP1
14.6
ferrocyanide
-
MnP1 from mutant F190A
0.74
guaiacol
-
-
0.74
guaiacol
-
in 0.05 M malonate-NaOH buffer pH 4.0 containing 0.2 mM H2O2, 45°C
0.75
guaiacol
pH 7.4, 30°C, recombinant wild-type enzyme, in absence of Mn2+
8.92
guaiacol
-
in 0.05 M malonate-NaOH buffer pH 4.0 containing 0.2 mM H2O2 and 1.5 mM Mn2+, 45°C
11.26
guaiacol
pH 4.0, 30°C, recombinant wild-type enzyme, in absence of Mn2+
14.5
guaiacol
-
Mn2+-independent activity
17.3
guaiacol
-
30°C, pH 4.5
20.54
guaiacol
pH 7.4, 30°C, recombinant wild-type enzyme, in presence of Mn2+
26.15
guaiacol
pH 4.0, 30°C, recombinant mutant E166D, in absence of Mn2+
31.7
guaiacol
-
Mn2+-dependent activity
40
guaiacol
-
isoenzyme H5
53
guaiacol
-
isoenzyme H3
56
guaiacol
-
isoenzyme H4
64.57
guaiacol
pH 4.0, 30°C, recombinant mutant E166Q, in absence of Mn2+
136
guaiacol
pH 4.0, 30°C, recombinant mutant E166G, in absence of Mn2+
142
guaiacol
pH 4.0, 30°C, recombinant wild-type enzyme, in presence of Mn2+
184
guaiacol
pH 4.0, 45°C, recombinant His6-tagged enzyme
17.3
H2O2
-
mutant R177E
121
H2O2
-
with 1.5 mM Mn2+ as cosubstrate, pH 5.5, 45°C
168
H2O2
-
recombinant enzyme
237
H2O2
-
wild-type enzyme
272.4
H2O2
-
reaction with Mn2+
879
H2O2
pH 4.5, 30°C, recombinant His6-tagged enzyme
5.3
Mn(II)
pH 5.0, 25°C, recombinant isozyme
6.7
Mn(II)
-
pH 4.5, 25°C
9.1
Mn(II)
pH 5.0, 25°C, recombinant isozyme
60.5
Mn(II)
mutant S168W, 25°C, pH 5.0
66.9
Mn(II)
mutant T162S/S168W/F258E/F262M/F268K/Q271N/S272R/S275G, 25°C, pH 5.0
83
Mn(II)
wild-type, 25°C, pH 5.0
369
Mn(II)
pH 4.5, 30°C, recombinant His6-tagged enzyme
0.22
Mn2+
-
-
24
Mn2+
-
A48C/A63C double mutant MnP after thermal treatment at 37°C for 15 min
161
Mn2+
Deuteromycotina sp.
-
-
240 - 290
Mn2+
-
MnP1 from mutants F190Y, F190L, F190I and F190A
242.4
Mn2+
-
with 0.2 mM H2O2 as cosubstrate, pH 5.5, 45°C
270 - 273
Mn2+
-
recombinant MnP and A48C/A63C double mutant MnP
290
Mn2+
-
wild-type MnP1
304.8
Mn2+
-
reactioin with H2O2
479.7
Mn2+
-
30°C, pH 4.5
39
Phenol red
-
isoenzyme H5
41
Phenol red
-
isoenzyme H4
66
Phenol red
-
isoenzyme H3
9.98
pyrogallol
-
-
9.98
pyrogallol
-
in 0.05 M malonate-NaOH buffer pH 4.0 containing 0.2 mM H2O2, 45°C
20.95
pyrogallol
-
in 0.05 M malonate-NaOH buffer pH 4.0 containing 0.2 mM H2O2 and 1.5 mM Mn2+, 45°C
0.06
Reactive Black 5
pH 3.0, 35°C, recombinant His6-tagged enzyme
7
Reactive Black 5
mutant T162S/S168W/F258E/F262M/F268K/Q271N/S272R/S275G, 25°C, pH 5.0
7.8
Reactive Black 5
mutant S168W, 25°C, pH 5.0
7.9
Reactive Black 5
pH 7.0, 30°C, mutant A172W/A269R
8.6
Reactive Black 5
pH 7.0, 30°C, mutant A172W
12.1
Reactive Black 5
pH 7.0, 30°C, mutant A172W/F259M
13.4
Reactive Black 5
pH 7.0, 30°C, mutant A172W/I171V
16
Reactive Black 5
pH 7.0, 30°C, mutant A172W/A273T
16.7
Reactive Black 5
pH 7.0, 30°C, mutant A172W/K168V
76
vanillylacetone
-
isoenzyme H5
90.3
vanillylacetone
-
isoenzyme H4
97
vanillylacetone
-
isoenzyme H3
0.3
veratryl alcohol
pH 7.0, 30°C, mutant A172W
0.3
veratryl alcohol
pH 7.0, 30°C, mutant A172W/A269R
0.45
veratryl alcohol
-
-
0.45
veratryl alcohol
-
in 0.05 M malonate-NaOH buffer pH 4.0 containing 0.2 mM H2O2 and 1.5 mM Mn2+, 45°C
0.54
veratryl alcohol
mutant S168W, 25°C, pH 5.0
0.7
veratryl alcohol
pH 7.0, 30°C, mutant A172W/I171V
1.1
veratryl alcohol
pH 7.0, 30°C, mutant A172W/A273T
1.1
veratryl alcohol
pH 7.0, 30°C, mutant A172W/F259M
1.3
veratryl alcohol
pH 7.0, 30°C, mutant A172W/K168V
1.69
veratryl alcohol
mutant T162S/S168W/F258E/F262M/F268K/Q271N/S272R/S275G, 25°C, pH 5.0
5.33
veratryl alcohol
-
Mn2+-independent activity
39.5
veratryl alcohol
-
Mn2+-dependent activity