Cloned (Comment) | Organism |
---|---|
cloning and sequencing of the gene encoding MnP2, promoter region is analyzed, nucleotide sequence of the cDNA encoding MnP1, mnp1 is located on chromosome IV, mnp2 on chromosome VI and mnp3 on chromosome V | Pleurotus ostreatus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.014 | - |
Mn2+ | MnP3 | Pleurotus ostreatus | |
0.0293 | 0.0294 | H2O2 | MnP2 and MnP3 | Pleurotus ostreatus | |
0.0838 | - |
Mn2+ | MnP2 | Pleurotus ostreatus | |
0.092 | - |
2,6-dimethoxyphenol | MnP3 | Pleurotus ostreatus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | - |
Pleurotus ostreatus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
38000 | - |
1 * 41000, MnP2, 1 * 38000, MnP3, SDS-PAGE | Pleurotus ostreatus |
41000 | - |
MnP2, gel filtration | Pleurotus ostreatus |
47000 | - |
MnP3, gel filtration | Pleurotus ostreatus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
Mn2+ + H+ + H2O2 | Pleurotus ostreatus | involved in lignin-degradation | Mn3+ + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pleurotus ostreatus | - |
white rot basidomycete, 3 isoenzymes: MnP1, MnP2 and MnP3 | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | presence of high-mannose-type glucans | Pleurotus ostreatus |
Purification (Comment) | Organism |
---|---|
MnP2 and MnP3, purified from poplar culture | Pleurotus ostreatus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Pleurotus ostreatus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,6-dimethoxyphenol + H2O2 | - |
Pleurotus ostreatus | ? | - |
? | |
Mn2+ + H+ + H2O2 | - |
Pleurotus ostreatus | Mn3+ + H2O | Mn3+ oxidizes 2,6-dimethoxyphenol | ? | |
Mn2+ + H+ + H2O2 | - |
Pleurotus ostreatus | Mn3+ + H2O | Mn3+ oxidizes syringaldazine | ? | |
Mn2+ + H+ + H2O2 | - |
Pleurotus ostreatus | Mn3+ + H2O | Mn3+ oxidizes guaiacol | ? | |
Mn2+ + H+ + H2O2 | involved in lignin-degradation | Pleurotus ostreatus | Mn3+ + H2O | - |
? | |
additional information | enzyme oxidizes 2,2-azino-di-3-ethylbenzothiazoline-6-sulfonate | Pleurotus ostreatus | ? | - |
? | |
additional information | no activity with veratryl alcohol | Pleurotus ostreatus | ? | - |
? | |
additional information | Mn2+-dependent and Mn2+-independent peroxidase activities when tested on the phenolic substrates 2,6-dimethoxyphenol, 2,2-azino-di-3-ethylbenzothiazoline-6-sulfonate, guaiacol and syringaldazine, more rapid oxidation in presence of Mn2+ | Pleurotus ostreatus | ? | - |
? | |
additional information | enzyme oxidizes 2,6-dimethoxyphenol | Pleurotus ostreatus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 41000, MnP2, 1 * 38000, MnP3, SDS-PAGE | Pleurotus ostreatus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Pleurotus ostreatus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
100 | - |
Mn2+ | MnP2 | Pleurotus ostreatus | |
100 | - |
H2O2 | MnP2 | Pleurotus ostreatus | |
200 | - |
2,6-dimethoxyphenol | MnP3 | Pleurotus ostreatus | |
230 | - |
Mn2+ | MnP3 | Pleurotus ostreatus | |
230 | - |
H2O2 | MnP3 | Pleurotus ostreatus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
MnP2: pI 3.7, MnP3: pI 3.5 | Pleurotus ostreatus |
2.5 | - |
oxidation of 2,6-dimethoxyphenol in absence of Mn2+ | Pleurotus ostreatus |
4 | - |
oxidation of Mn2+ | Pleurotus ostreatus |
4.5 | - |
oxidation of 2,6-dimethoxyphenol in presence of Mn2+ | Pleurotus ostreatus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
heme | iron protoporphyrin IX prosthetic group | Pleurotus ostreatus |