1.11.1.13: manganese peroxidase

This is an abbreviated version!
For detailed information about manganese peroxidase, go to the full flat file.

Word Map on EC 1.11.1.13

Reaction

2 Mn(II) + 2 H+ +

H2O2
= 2 Mn(III) + 2 H2O

Synonyms

hybrid Mn-peroxidase, L-MnP, LeMnP2, manganese peroxidase, manganese-dependent peroxidase, Mn-dependent (NADH-oxidizing) peroxidase, Mn2+: hydrogen peroxide oxidoreductase, Mn2+:hydrogen peroxide oxidoreductase, MnP, MnP 1, MnP II, MnP-GY, MnP-PGY, mnp1, MnP2, MnP3, MnP6, MP, multifunctional manganese peroxidase, Nf b19 MNP2, peroxidase, manganese, peroxidase-M2

ECTree

     1 Oxidoreductases
         1.11 Acting on a peroxide as acceptor
             1.11.1 Peroxidases
                1.11.1.13 manganese peroxidase

Purification

Purification on EC 1.11.1.13 - manganese peroxidase

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PURIFICATION/commentary
ORGANISM
UNIPROT
LITERATURE
11.1fold purification
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12fold purification; 3 isozymes
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148fold purification of MnP1 and 157fold purification of MnP2
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2 isoenzymes: BOS1 and BOS2
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24fold purification
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25fold purification; MnP1 and MnP2
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3 isoenzymes
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35.8fold purification
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8.1-16fold purification
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affinity chromatography on blue agarose: isozyme H4 95% pure, followed by preparative isoelectric focusing: isozymes H3 and H5 pure
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enzymes are extracted with 50 mM citrate-phosphate buffer at pH 4.0, 5.0 and 6.0 at 10°C and 25°C
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extract is loaded onto a HiPREP26/10 desalting column followd by chromatography on a DEAE-Sepharose and a MonoQ HR5/5 column, finally the active fractions are applied to CIM QA Disks
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from Ceriporiopsis subvermispora by gel filtration and chromatography on a Mono-Q column
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from stem juice
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isoenzyme H4; purification of recombinant A48C/A63C double mutant MnP, expressed in Escherichia coli
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isoenzymes H4 and H5
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MnP1 and MnP2
MnP1: 19fold purification
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MnP2 and MnP3, purified from poplar culture
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MnPI, MnPII and MnPIII
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partial purification
partial purification, MnP2 and MnP3 are not separated
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purification of a protein complex containing MnP, laccase and beta-glucosidase activities
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purification of MnPs from the mutants F190Y, F190L, F190I and F190A
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purification of recombinant MnP, expressed in Escherichia coli
purification of recombinant MnP, expressed in Phanerochaete chrysosporium
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purification of the mutant enzymes R177D, R177E, R177N and R177Q
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purification scheme involves ultrafiltration, affinity chromatography on concanavalin-A Sepharose and gel filtration; ultrafiltration, Concanavalin-A Sepharose column chromatography and Superdex 75 gel filtration
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using a Toyopearl Butyl-650M, a Toyopearl DEAE-650 and a Superdex 75 HR 10/30 column
B5U990