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Literature summary for 1.11.1.13 extracted from

  • Duan, Z.; Shen, R.; Liu, B.; Yao, M.; Jia, R.
    Comprehensive investigation of a dye-decolorizing peroxidase and a manganese peroxidase from Irpex lacteus F17, a lignin-degrading basidiomycete (2018), AMB Express, 8, 119 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.11.1.13

Cloned(Commentary)

Cloned (Comment) Organism
gene Il-MnP6, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression of His6-tagged enzyme in Escherichia coli strain Rosetta (DE3) in inclusion bodies Irpex lacteus

Inhibitors

Inhibitors Comment Organism Structure
2-mercaptoethanol 85% inhibition at 0.1 mM, complete inhibition at 1 mM Irpex lacteus
Al3+ 8% inhibition at 1 mM, 78% inhibition at 10 mM Irpex lacteus
Ca2+ 10% inhibition at 10 mM Irpex lacteus
Cd2+ 49% inhibition at 10 mM Irpex lacteus
Cu2+ 50% inhibition at 10 mM Irpex lacteus
DTT 75% inhibition at 0.1 mM, complete inhibition at 1 mM Irpex lacteus
EDTA 92% inhibition at 1 mM, 96% inhibition at 10 mM Irpex lacteus
Fe2+ 81% inhibition at 0.1 mM, complete inhibition at 1 mM Irpex lacteus
Fe3+ 64% inhibition at 1 mM, complete inhibition at 10 mM Irpex lacteus
H2O2 the enzyme activity is reduced by 10% as the H2O2 concentration increases from 0.1 to 4 mM Irpex lacteus
Ni2+ 8% inhibition at 10 mM Irpex lacteus
Thiourea 81% inhibition at 10 mM Irpex lacteus
Zn2+ 15% inhibition at 10 mM Irpex lacteus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0026
-
 guaiacol pH 4.0, 45°C, recombinant His6-tagged enzyme Irpex lacteus
0.0044
-
 Reactive Black 5 pH 3.0, 35°C, recombinant His6-tagged enzyme Irpex lacteus
0.053
-
 2,6-dimethoxyphenol pH 4.5, 55°C, recombinant His6-tagged enzyme Irpex lacteus
0.081
-
 Reactive Blue 19 pH 3.0, 35°C, recombinant His6-tagged enzyme Irpex lacteus
0.083
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) pH 3.5, 45°C, recombinant His6-tagged enzyme Irpex lacteus
0.129
-
 Mn(II) pH 4.5, 30°C, recombinant His6-tagged enzyme Irpex lacteus
0.194
-
 H2O2 pH 4.5, 30°C, recombinant His6-tagged enzyme Irpex lacteus

Metals/Ions

Metals/Ions Comment Organism Structure
Ba2+ 15% activation at 10 mM Irpex lacteus
Co2+ 11% activation at 10 mM Irpex lacteus
additional information poor effect on enzyme activity by Mn2+, Na+, and Mg2+ at 0.1-10 mM Irpex lacteus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2 Mn(II) + 2 H+ + H2O2 Irpex lacteus
-
 2 Mn(III) + 2 H2O
-
 ?
2 Mn(II) + 2 H+ + H2O2 Irpex lacteus F17
-
 2 Mn(III) + 2 H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Irpex lacteus A0A2P1C6N7
-
-
Irpex lacteus F17 A0A2P1C6N7
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant refolded His6-tagged enzyme expressed from Escherichia coli strain Rosetta (DE3) by dialysis and nickel affinity chromatography Irpex lacteus

Renatured (Commentary)

Renatured (Comment) Organism
recombinant His6-tagged isozyme MnP6 from Escherichia coli strain Rosetta(DE3) inclusion bodies, refolding conditions: 1.5 M urea, 150 mM CaCl2, 0.025 mM hemin, 10% glycerol, 0.5 mM GSSG, 0.05 mM MnSO4, 20 mM KCl, and 50 mM Tris-HCl, pH 8.5 Irpex lacteus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
23.8
-
 pH 4.5, 30°C, purified recombinant refolded His6-tagged enzyme Irpex lacteus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 Mn(II) + 2 H+ + H2O2
-
 Irpex lacteus 2 Mn(III) + 2 H2O
-
 ?
2 Mn(II) + 2 H+ + H2O2
-
 Irpex lacteus F17 2 Mn(III) + 2 H2O
-
 ?
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + 2 H+ + H2O2 ABTS Irpex lacteus oxidized 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + 2 H2O
-
 ?
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + 2 H+ + H2O2 ABTS Irpex lacteus F17 oxidized 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + 2 H2O
-
 ?
2,6-dimethoxyphenol + 2 H+ + H2O2
-
 Irpex lacteus oxidized 2,6-dimethoxyphenol + 2 H2O
-
 ?
2,6-dimethoxyphenol + 2 H+ + H2O2
-
 Irpex lacteus F17 oxidized 2,6-dimethoxyphenol + 2 H2O
-
 ?
guaiacol + 2 H+ + H2O2
-
 Irpex lacteus oxidized guaiacol + 2 H2O
-
 ?
guaiacol + 2 H+ + H2O2
-
 Irpex lacteus F17 oxidized guaiacol + 2 H2O
-
 ?
additional information no activity with veratryl alcohol (VA) Irpex lacteus ?
-
-
additional information no activity with veratryl alcohol (VA) Irpex lacteus F17 ?
-
-
Reactive Black 5 + 2 H+ + H2O2 dye decolorization Irpex lacteus Reactive Black 5 + 2 H2O
-
 ?
Reactive Blue 19 + 2 H+ + H2O2 dye decolorization Irpex lacteus oxidized Reactive Blue 19 + 2 H2O
-
 ?

Subunits

Subunits Comment Organism
? x * 44600, recombinant refolded His6-tagged enzyme, SDS-PAGE, x * 38200, about, MnP6, sequence calculation Irpex lacteus
More enzyme secondary structure analysis overview Irpex lacteus

Synonyms

Synonyms Comment Organism
Il-MnP6
-
 Irpex lacteus
MnP
-
 Irpex lacteus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 recombinant enzyme Irpex lacteus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
 purified recombinant refolded His6-tagged enzyme retaining over 90% of its enzymatic activity below 45°C for 12 h Irpex lacteus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.06
-
 Reactive Black 5 pH 3.0, 35°C, recombinant His6-tagged enzyme Irpex lacteus
7.3
-
 Reactive Blue 19 pH 3.0, 35°C, recombinant His6-tagged enzyme Irpex lacteus
184
-
 guaiacol pH 4.0, 45°C, recombinant His6-tagged enzyme Irpex lacteus
245
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) pH 3.5, 45°C, recombinant His6-tagged enzyme Irpex lacteus
369
-
 Mn(II) pH 4.5, 30°C, recombinant His6-tagged enzyme Irpex lacteus
879
-
 H2O2 pH 4.5, 30°C, recombinant His6-tagged enzyme Irpex lacteus
989
-
 2,6-dimethoxyphenol pH 4.5, 55°C, recombinant His6-tagged enzyme Irpex lacteus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5
-
 optimum peak 1 Irpex lacteus
7
-
 optimum peak 2 Irpex lacteus

pH Stability

pH Stability pH Stability Maximum Comment Organism
3.5 7.5 purified recombinant refolded His6-tagged enzyme retaining 75% of maximal activity within this range Irpex lacteus

Cofactor

Cofactor Comment Organism Structure
heme
-
 Irpex lacteus

General Information

General Information Comment Organism
evolution the enzyme belongs to the ligninolytic peroxidase gene family, 13 MnP genes and five dye-decolorizing peroxidase (DyP) genes are present in the Irpex lacteus strain F17 genome, they represent two different families of heme peroxidases and are unrelated to the fungal class II peroxidases Irpex lacteus
physiological function the manganese peroxidase of Irpex lacteus strain 17 is able to degrade recalcitrant aromatic pollutants Irpex lacteus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
16.67
-
 Reactive Black 5 pH 3.0, 35°C, recombinant His6-tagged enzyme Irpex lacteus
90.12
-
 Reactive Blue 19 pH 3.0, 35°C, recombinant His6-tagged enzyme Irpex lacteus
2860.5
-
 Mn(II) pH 4.5, 30°C, recombinant His6-tagged enzyme Irpex lacteus
2951.8
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) pH 3.5, 45°C, recombinant His6-tagged enzyme Irpex lacteus
4530.9
-
 H2O2 pH 4.5, 30°C, recombinant His6-tagged enzyme Irpex lacteus
18660.4
-
 2,6-dimethoxyphenol pH 4.5, 55°C, recombinant His6-tagged enzyme Irpex lacteus
70769
-
 guaiacol pH 4.0, 45°C, recombinant His6-tagged enzyme Irpex lacteus