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Literature summary for 1.11.1.13 extracted from

  • Glenn, J.K.; Akileswaran, L.; Gold, M.H.
    Mn(II) oxidation is the principal function of the extracellular Mn-peroxidase from Phanerochaete chrysosporium (1986), Arch. Biochem. Biophys., 251, 688-696.
    View publication on PubMed
Search for more literature for 1.11.1.13

Activating Compound

Activating Compound Comment Organism Structure
alpha-hydroxy acid activates by chelating and stabilizing Mn3+ rather than activating the enzyme Phanerodontia chrysosporium
H2O2 H2O2-dependent Phanerodontia chrysosporium
Lactate stimulates by complexing with and stabilizing Mn3+ Phanerodontia chrysosporium

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.08
-
 Mn2+
-
 Phanerodontia chrysosporium
0.14
-
 H2O2
-
 Phanerodontia chrysosporium

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Phanerodontia chrysosporium
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
46000
-
-
 Phanerodontia chrysosporium

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
Mn2+ + H+ + H2O2 Phanerodontia chrysosporium involved in lignin-degradation, the mechanism enables the fungus to oxidize structures within woods which are inaccessible to enzymes Mn3+ + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Phanerodontia chrysosporium
-
 white rot basidomycete
-

Purification (Commentary)

Purification (Comment) Organism
-
 Phanerodontia chrysosporium

Reaction

Reaction Comment Organism Reaction ID
2 Mn(II) + 2 H+ + H2O2 = 2 Mn(III) + 2 H2O mechanism Phanerodontia chrysosporium
a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
180
-
 Mn3+-lactate complex formation Phanerodontia chrysosporium

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Co2+ + H+ + H2O2 reduction of enzyme compound II, oxidation at 2% the rate of Mn2+ oxidation Phanerodontia chrysosporium Co3+ + H2O
-
 ?
Mn2+ + H+ + H2O2 specifically oxidizes Mn2+ Phanerodontia chrysosporium Mn3+ + H2O Mn3+ complexed to lactate or other alpha-hydroxy acids acts as an obligatory oxidation intermediate in the oxidation of various dyes and lignin model compounds, Mn3+-lactate complex oxidizes all dyes oxidized by the enzyme in presence of Mn2+: NADH, pinacyanol, phenol red and poly B-411 ?
Mn2+ + H+ + H2O2 specifically oxidizes Mn2+ Phanerodontia chrysosporium Mn3+ + H2O the diffusible product is Mn3+ ?
Mn2+ + H+ + H2O2 specifically oxidizes Mn2+ Phanerodontia chrysosporium Mn3+ + H2O chelation of Mn3+ by organic acids stabilizes Mn3+ at a high redox potential ?
Mn2+ + H+ + H2O2 in presence of H2O2 enzyme oxidizes Mn2+ significantly faster than all other substrates, main function of enzyme is oxidation of Mn2+ to Mn3+ Phanerodontia chrysosporium Mn3+ + H2O Mn3+ complexed to lactate or other alpha-hydroxy acids acts as an obligatory oxidation intermediate in the oxidation of various dyes and lignin model compounds, Mn3+-lactate complex oxidizes all dyes oxidized by the enzyme in presence of Mn2+: NADH, pinacyanol, phenol red and poly B-411 ?
Mn2+ + H+ + H2O2 in presence of H2O2 enzyme oxidizes Mn2+ significantly faster than all other substrates, main function of enzyme is oxidation of Mn2+ to Mn3+ Phanerodontia chrysosporium Mn3+ + H2O the diffusible product is Mn3+ ?
Mn2+ + H+ + H2O2 in presence of H2O2 enzyme oxidizes Mn2+ significantly faster than all other substrates, main function of enzyme is oxidation of Mn2+ to Mn3+ Phanerodontia chrysosporium Mn3+ + H2O chelation of Mn3+ by organic acids stabilizes Mn3+ at a high redox potential ?
Mn2+ + H+ + H2O2 involved in lignin-degradation, the mechanism enables the fungus to oxidize structures within woods which are inaccessible to enzymes Phanerodontia chrysosporium Mn3+ + H2O
-
 ?
additional information enzyme oxidizes a variety of organic compounds in presence, but not in absence of Mn2+ Phanerodontia chrysosporium ?
-
 ?
additional information in absence of Mn2+ the enzyme oxidizes pinacyanol as most easily oxidized dye at 1.7% of the rate of the Mn2+ oxidation Phanerodontia chrysosporium ?
-
 ?
additional information enzyme oxidizes 2,2-azino-di-3-ethylbenzothiazoline-6-sulfonate Phanerodontia chrysosporium ?
-
 ?
additional information in absence of H2O2 the enzyme oxidizes Mn-dependently NADH to NAD+, generating H2O2 for oxidizing other substrates Phanerodontia chrysosporium ?
-
 ?
additional information Mn2+-dependent oxidation of 2,2-azino-di-3-ethylbenzothiazoline-6-sulfonate Phanerodontia chrysosporium ?
-
 ?
additional information in absence of H2O2 the enzyme oxidizes Mn-dependently NADPH+ to NADP+ Phanerodontia chrysosporium ?
-
 ?
additional information enzyme oxidizes phenol red Phanerodontia chrysosporium ?
-
 ?
additional information enzyme oxidizes the polymeric dyes poly R-481 and poly B-411 Phanerodontia chrysosporium ?
-
 ?
additional information in presence of Mn2+ enzyme oxidizes various organic compounds Phanerodontia chrysosporium ?
-
 ?
additional information no oxidation of Ni2+ Phanerodontia chrysosporium ?
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5
-
 oxidation of 2,2-azino-bis-3-ethyl-6-benzothiazolinesulfonate Phanerodontia chrysosporium
5
-
 Mn3+-lactate complex formation Phanerodontia chrysosporium

pH Range

pH Minimum pH Maximum Comment Organism
4 5.5 about half-maximal activity at pH 4.0 and 5.5, 2,2-azino-bis-3-ethyl-6-benzothiazolinesulfonate-oxidation Phanerodontia chrysosporium
4.5 5.7 about 65% of maximal activity at pH 4.5 and about 50% at pH 5.7, Mn(III)-lactate formation Phanerodontia chrysosporium

Cofactor

Cofactor Comment Organism Structure
heme heme protein Phanerodontia chrysosporium