1.1.1.431: D-xylose reductase (NADPH)

This is an abbreviated version!
For detailed information about D-xylose reductase (NADPH), go to the full flat file.

Reaction

Synonyms

ALR1, Cb-XR, CbXR, CtXR, D-xylose reductase, D-xylose reductase 1, D-xylose reductase 2, D-xylose reductase 3, DnXR, GRE3, monospecific xylose reductase, More, msXR, NAD(P)H-dependent D-xylose reductase, NAD(P)H-dependent xylose reductase, NADPH dependent D-xylose reductase, NADPH-dependent D-xylose reductase II,III, NADPH-dependent xylose reductase, NADPH-preferring xylose reductase, NRRL3_10868, SsXR, Texr, TrxR, XR1, XR2, XR3, XRTL, XYL1, xylose reductase, xyr8, XyrA, XyrB

ECTree

1 Oxidoreductases

1.1 Acting on the CH-OH group of donors

1.1.1 With NAD⁺ or NADP⁺ as acceptor

1.1.1.431 D-xylose reductase (NADPH)

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Results	in table
5	Activating Compound
16	AA Sequence
10	Application
22	Cloned(Commentary)
64	Cofactor
7	Crystallization (Commentary)
6	Expression
33	General Information
2	General Stability
10	IC50 Value
39	Inhibitors
81	kcat/KM [mM/s]
18	Ki Value [mM]
97	KM Value [mM]
1	Localization
4	Metals/Ions
15	Molecular Weight [Da]
44	Natural Substrates/ Products (Substrates)
113	Organism
1	Pathway
23	pH Optimum
3	pH Range
7	pH Stability
6	pI Value
2	Posttranslational Modification
49	Protein Variants
17	Purification (Commentary)
4	Reaction
65	Reference
3	Source Tissue
20	Specific Activity [micromol/min/mg]
6	Storage Stability
170	Substrates and Products (Substrate)
29	Subunits
99	Synonyms
1	Systematic Name
22	Temperature Optimum [°C]
4	Temperature Range [°C]
8	Temperature Stability [°C]
75	Turnover Number [1/s]

Purification

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Purification on EC 1.1.1.431 - D-xylose reductase (NADPH)

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from mycelium

identification of the most suitable operating conditions for purification, the aqueous two-phase systems proves effective for partial purification of xylose reductase in cell-free crude extract

Debaryomyces hansenii

recombinant enzyme

recombinant enzyme from Pichia pastoris strain GS115 to homogeneity with an overall 13.77fold increase in specific activity, by ammonium sulfate fractionation, ion exchange chromatography, and gel filtration

Thermomyces lanuginosus

A0A455KZK8

760720

recombinant His-tagged enzyme from Escherichia coli strain L21(DE3)pLysS by nickel affinity chromatography and ultrafiltration

Aspergillus niger

760777

recombinant His-tagged enzyme from Escherichia coli strain Rosetta

Debaryomyces nepalensis

A0A0M4HL56

761017

recombinant His-tagged enzyme from Escherichia coli strain Rosetta by nickel affinity chromatography

Debaryomyces nepalensis

A0A0M4HL56

761066

recombinant His-tagged enzyme from Escherichia coli strain UT5600 by nickel affinity chromatography

Zymomonas mobilis subsp. mobilis

F2YCN5

721903

recombinant His6-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3)-T1R by nickel affinity chromatography and gel filtration

Scheffersomyces stipitis

P31867

762450

wild-type and mutant enzyme Y49F

Saccharomyces cerevisiae

701424

yeast cell extract is fractionated by biomimetic chromatography using the dye HE-3B (reactive Red 120) as pseudoaffinity ligand coupled to Sepharose CL-4B. In the second step, high-resolution anion-exchange chromatography using Mono Q yields purified isoform ALR2 in overall yield of 62%

[Candida] intermedia

765140