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Literature summary for 1.1.1.431 extracted from

  • Wang, X.; Fang, B.; Luo, J.; Li, W.; Zhang, L.
    Heterologous expression, purification, and characterization of xylose reductase from Candida shehatae (2007), Biotechnol. Lett., 29, 1409-1412.
    View publication on PubMed
Search for more literature for 1.1.1.431

Activating Compound

Activating Compound Comment Organism Structure
bovine serum albumin 1 mg/ml. the specific activity is increased by 20-30% Scheffersomyces shehatae
EDTA 1 mM, 30% activation Scheffersomyces shehatae
Triton X-100 0.1%, the specific activity is increased by 20–30% Scheffersomyces shehatae
Tween 20 0.1%, the specific activity is increased by 20-30% Scheffersomyces shehatae
Tween 80 0.1%, the specific activity is increased by 20-30% Scheffersomyces shehatae

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli as a His6-tagged fusion protein Scheffersomyces shehatae

Inhibitors

Inhibitors Comment Organism Structure
DTT 1 mM, 35% inhibition Scheffersomyces shehatae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.02
-
 NADPH pH 6.5, 35°C Scheffersomyces shehatae
0.15
-
 NADH pH 6.5, 35°C Scheffersomyces shehatae
160
-
 D-xylose pH 6.5, 35°C Scheffersomyces shehatae

Metals/Ions

Metals/Ions Comment Organism Structure
Li+ Ca2+, Li+, Mg2+, Mn2+ and NH4+ at 10 mM decrease activity by 10-50% Scheffersomyces shehatae
Mg2+ Ca2+, Li+, Mg2+, Mn2+ and NH4+ at 10 mM decrease activity by 10-50% Scheffersomyces shehatae
Mn2+ Ca2+, Li+, Mg2+, Mn2+ and NH4+ at 10 mM decrease activity by 10-50% Scheffersomyces shehatae
NH4+ Ca2+, Li+, Mg2+, Mn2+ and NH4+ at 10 mM decrease activity by 10-50% Scheffersomyces shehatae

Organism

Organism UniProt Comment Textmining
Scheffersomyces shehatae A0MTG4
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Scheffersomyces shehatae

Storage Stability

Storage Stability Organism
4°C, enzyme retains activity for several months Scheffersomyces shehatae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-galactose + NADH + H+ 48% of the activity compared to D-xylose (with NADH as cofactor) Scheffersomyces shehatae ?
-
 ?
D-glucose + NADH + H+ 10 of the activity compared to D-xylose (with NADH as cofactor) Scheffersomyces shehatae ?
-
 ?
D-mannose + NADH + H+ 8% of the activity compared to D-xylose (with NADH as cofactor) Scheffersomyces shehatae ?
-
 ?
D-xylose + NADH + H+ NADPH is the preferred cofactor Scheffersomyces shehatae xylitol + NAD+
-
 ?
D-xylose + NADPH + H+ NADPH is the preferred cofactor, specific for D-xylose Scheffersomyces shehatae xylitol + NADP+
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35 40
-
 Scheffersomyces shehatae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
 with NADH as cofactor Scheffersomyces shehatae

Cofactor

Cofactor Comment Organism Structure
NADH NADPH is the preferred cofactor Scheffersomyces shehatae
NADPH NADPH is the preferred cofactor Scheffersomyces shehatae