1.1.1.431: D-xylose reductase (NADPH)

This is an abbreviated version!
For detailed information about D-xylose reductase (NADPH), go to the full flat file.

Reaction

Synonyms

ALR1, Cb-XR, CbXR, CtXR, D-xylose reductase, D-xylose reductase 1, D-xylose reductase 2, D-xylose reductase 3, DnXR, GRE3, monospecific xylose reductase, More, msXR, NAD(P)H-dependent D-xylose reductase, NAD(P)H-dependent xylose reductase, NADPH dependent D-xylose reductase, NADPH-dependent D-xylose reductase II,III, NADPH-dependent xylose reductase, NADPH-preferring xylose reductase, NRRL3_10868, SsXR, Texr, TrxR, XR1, XR2, XR3, XRTL, XYL1, xylose reductase, xyr8, XyrA, XyrB

ECTree

     1 Oxidoreductases

         1.1 Acting on the CH-OH group of donors

             1.1.1 With NAD⁺ or NADP⁺ as acceptor

                1.1.1.431 D-xylose reductase (NADPH)

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Results	in table
5	Activating Compound
16	AA Sequence
10	Application
22	Cloned(Commentary)
64	Cofactor
7	Crystallization (Commentary)
6	Expression
33	General Information
2	General Stability
10	IC50 Value
39	Inhibitors
81	kcat/KM [mM/s]
18	Ki Value [mM]
97	KM Value [mM]
1	Localization
4	Metals/Ions
15	Molecular Weight [Da]
44	Natural Substrates/ Products (Substrates)
113	Organism
1	Pathway
23	pH Optimum
3	pH Range
7	pH Stability
6	pI Value
2	Posttranslational Modification
49	Protein Variants
17	Purification (Commentary)
4	Reaction
65	Reference
3	Source Tissue
20	Specific Activity [micromol/min/mg]
6	Storage Stability
170	Substrates and Products (Substrate)
29	Subunits
99	Synonyms
1	Systematic Name
22	Temperature Optimum [°C]
4	Temperature Range [°C]
8	Temperature Stability [°C]
75	Turnover Number [1/s]

KM Value

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KM Value on EC 1.1.1.431 - D-xylose reductase (NADPH)

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KM VALUE [mM]

SUBSTRATE

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

0.221

2-deoxy-D-galactose

[Candida] intermedia

-

pH 7.0, 25°C, NADPH-dependent monospecific xylose reductase, cofactor: NADPH

764945

0.058

2-deoxy-D-glucose

[Candida] intermedia

-

pH 7.0, 25°C, NADPH-dependent monospecific xylose reductase, cofactor: NADPH

764945

0.038

2-deoxy-D-ribose

[Candida] intermedia

-

pH 7.0, 25°C, NADPH-dependent monospecific xylose reductase, cofactor: NADPH

764945

1.5 - 4.4

5,6-dideoxy-5,6-difluoro-D-glucofuranose

2 entries

-

0.4 - 3

5,6-dideoxy-5-fluoro-D-glucofuranose

2 entries

-

1 - 10.4

5,6-dideoxy-6-fluoro-D-xylo-hexofuranose

2 entries

-

0.9 - 2.2

5,6-dideoxy-D-xylo-hexofuranose

2 entries

-

4

5-azido-5-deoxy-D-xylofuranose

Saitozyma flava

-

pH 7, 25°C

764095

-

0.7 - 1

5-deoxy-D-xylofuranose

2 entries

-

1 - 1.6

5-fluoro-5-deoxy-D-xylofuranose

2 entries

-

0.6 - 1.3

6-azido-5,6-dideoxy-5-fluoro-D-glucofuranose

2 entries

-

1.8

benzaldehyde

Zymomonas mobilis subsp. mobilis

F2YCN5

pH 7.2, temperature not specified in the publication

721903

2 - 3

Butanal

[Candida] intermedia

-

pH 7.0, 25°C, NADPH-dependent monospecific xylose reductase, cofactor: NADPH

764945

0.32 - 9.8

cyclohexanecarboxaldehyde

2 entries

0.033 - 5.5

D-erythrose

2 entries

0.01

D-fucose

[Candida] intermedia

-

pH 7.0, 25°C, NADPH-dependent monospecific xylose reductase, cofactor: NADPH

764945

0.061 - 303

D-galactose

4 entries

0.006 - 177

D-glucose

2 entries

0.091 - 36.3

D-ribose

2 entries

0.016 - 258

D-xylose

20 entries

1.14

DL-glyceraldehyde

[Candida] intermedia

-

pH 7.0, 25°C, NADPH-dependent monospecific xylose reductase, cofactor: NADPH

764945

4.2

furfural

Zymomonas mobilis subsp. mobilis

F2YCN5

pH 7.2, temperature not specified in the publication

721903

0.02 - 93

L-arabinose

4 entries

0.117

L-Lyxose

[Candida] intermedia

-

pH 7.0, 25°C, NADPH-dependent monospecific xylose reductase, cofactor: NADPH

764945

0.0187 - 40

NADH

6 entries

0.016

NADP+

[Candida] intermedia

-

pH 7, 25°C

764945

0.0073 - 7.6

NADPH

15 entries

0.6 - 2.5

oenanthaldehyde

2 entries

-

3.9

pentanal

[Candida] intermedia

-

pH 7.0, 25°C, NADPH-dependent monospecific xylose reductase, cofactor: NADPH

764945

78

propionaldehyde

[Candida] intermedia

-

pH 7.0, 25°C, NADPH-dependent monospecific xylose reductase, cofactor: NADPH

764945

334 - 537

xylitol

3 entries

additional information

additional information

8 entries

-

1.5

5,6-dideoxy-5,6-difluoro-D-glucofuranose

[Candida] intermedia

-

pH 7, 25°C

764095

-

4.4

5,6-dideoxy-5,6-difluoro-D-glucofuranose

Saitozyma flava

-

pH 7, 25°C

764095

-

0.4

5,6-dideoxy-5-fluoro-D-glucofuranose

Saitozyma flava

-

pH 7, 25°C

764095

-

3

5,6-dideoxy-5-fluoro-D-glucofuranose

[Candida] intermedia

-

pH 7, 25°C

764095

-

1

5,6-dideoxy-6-fluoro-D-xylo-hexofuranose

Saitozyma flava

-

pH 7, 25°C

764095

-

10.4

5,6-dideoxy-6-fluoro-D-xylo-hexofuranose

[Candida] intermedia

-

pH 7, 25°C

764095

-

0.9

5,6-dideoxy-D-xylo-hexofuranose

Saitozyma flava

-

pH 7, 25°C

764095

-

2.2

5,6-dideoxy-D-xylo-hexofuranose

[Candida] intermedia

-

pH 7, 25°C

764095

-

0.7

5-deoxy-D-xylofuranose

Saitozyma flava

-

pH 7, 25°C

764095

-

1

5-deoxy-D-xylofuranose

[Candida] intermedia

-

pH 7, 25°C

764095

-

1

5-fluoro-5-deoxy-D-xylofuranose

Saitozyma flava

-

pH 7, 25°C

764095

-

1.6

5-fluoro-5-deoxy-D-xylofuranose

[Candida] intermedia

-

pH 7, 25°C

764095

-

0.6

6-azido-5,6-dideoxy-5-fluoro-D-glucofuranose

Saitozyma flava

-

pH 7, 25°C

764095

-

1.3

6-azido-5,6-dideoxy-5-fluoro-D-glucofuranose

[Candida] intermedia

-

pH 7, 25°C

764095

-

0.32

cyclohexanecarboxaldehyde

[Candida] intermedia

-

pH 7, 25°C

764095

9.8

cyclohexanecarboxaldehyde

Saitozyma flava

-

pH 7, 25°C

764095

0.033

D-erythrose

[Candida] intermedia

-

pH 7.0, 25°C, NADPH-dependent monospecific xylose reductase, cofactor: NADPH

764945

5.5

D-erythrose

Debaryomyces nepalensis

A0A0M4HL56

recombinant His-tagged enzyme, pH 7.0, 45°C

761066

0.061

D-galactose

[Candida] intermedia

-

pH 7.0, 25°C, NADPH-dependent monospecific xylose reductase, cofactor: NADPH

764945

51

D-galactose

Saitozyma flava

-

pH 7, 25°C

764095

72.2

D-galactose

Rhizomucor pusillus

I7HD84

pH 7.0, 30°C

740777

303

D-galactose

[Candida] intermedia

-

pH 7, 25°C

764095

0.006

D-glucose

[Candida] intermedia

-

pH 7.0, 25°C, NADPH-dependent monospecific xylose reductase, cofactor: NADPH

764945

177

D-glucose

Rhizomucor pusillus

I7HD84

pH 7.0, 30°C

740777

0.091

D-ribose

[Candida] intermedia

-

pH 7.0, 25°C, NADPH-dependent monospecific xylose reductase, cofactor: NADPH

764945

36.3

D-ribose

Rhizomucor pusillus

I7HD84

pH 7.0, 30°C

740777

0.016

D-xylose

[Candida] intermedia

-

pH 7.0, 25°C, NADPH-dependent monospecific xylose reductase, cofactor: NADPH

764945

0.064

D-xylose

Meyerozyma guilliermondii

-

pH 7.2, 25°C

763937

3.3

D-xylose

Aspergillus niger

-

recombinant enzyme, pH 7.0, 25°C

760777

14.8

D-xylose

Saccharomyces cerevisiae

-

pH 7.0, 22°C, wild-type enzyme

701424

15.36

D-xylose

Thermomyces lanuginosus

A0A455KZK8

recombinant enzyme, pH 6.5, 50°C

760720

27

D-xylose

Saitozyma flava

-

pH 7, 25°C

764095

28.1

D-xylose

Rhizomucor pusillus

I7HD84

pH 7.0, 30°C

740777

30

D-xylose

Candida tropicalis

-

pH 7.0, D-xylose reductase 2

699278

32.37

D-xylose

Scheffersomyces stipitis

P31867

with NADPH, without NaCl, pH 8.0, 30°C

762450

34

D-xylose

Candida tropicalis

-

pH 7.0, D-xylose reductase 3

699278

37

D-xylose

Candida tropicalis

-

pH 7.0, D-xylose reductase 1

699278

39

D-xylose

Aspergillus niger

-

30°C

668063

39.4

D-xylose

Scheffersomyces stipitis

P31867

with NADPH, with 150 mM NaCl, pH 8.0, 30°C

762450

39.61

D-xylose

Scheffersomyces stipitis

P31867

with NADH, without NaCl, pH 8.0, 30°C

762450

59.72

D-xylose

Scheffersomyces stipitis

P31867

with NADH, with 150 mM NaCl, pH 8.0, 30°C

762450

77

D-xylose

[Candida] intermedia

-

pH 7, 25°C

764945

81.8

D-xylose

Candida tropicalis

-

pH 7.0, 25°C

739881

82

D-xylose

[Candida] intermedia

-

pH 7, 25°C

764095, 765140

160

D-xylose

Scheffersomyces shehatae

A0MTG4

pH 6.5, 35°C

696892

258

D-xylose

Zymomonas mobilis subsp. mobilis

F2YCN5

pH 7.2, temperature not specified in the publication

721903

0.02

L-arabinose

[Candida] intermedia

-

pH 7.0, 25°C, NADPH-dependent monospecific xylose reductase, cofactor: NADPH

764945

9.9

L-arabinose

Aspergillus niger

-

recombinant enzyme, pH 7.0, 25°C

760777

41.7

L-arabinose

Rhizomucor pusillus

I7HD84

pH 7.0, 30°C

740777

93

L-arabinose

Aspergillus niger

-

30°C

668063

0.0187

NADH

Scheffersomyces stipitis

P31867

without NaCl, pH 8.0, 30°C

762450

0.136

NADH

Scheffersomyces stipitis

P31867

with 150 mM NaCl, pH 8.0, 30°C

762450

0.15

NADH

Scheffersomyces shehatae

A0MTG4

pH 6.5, 35°C

696892

0.35

NADH

Rhizomucor pusillus

I7HD84

pH 7.0, 30°C

740777

38

NADH

Yamadazyma tenuis

O74237

pH 7.0, 25°C, wild-type enzyme

696083

40

NADH

Yamadazyma tenuis

O74237

pH 7.0, 25°C, mutant enzyme D50A

696083

0.0073

NADPH

Candida tropicalis

-

pH 7.0, 25°C

739881

0.008

NADPH

Aspergillus niger

-

30°C, cosubstrate: D-xylose or L-arabinose

668063

0.009

NADPH

Candida tropicalis

-

pH 7.0, D-xylose reductase 3

699278

0.00928

NADPH

Scheffersomyces stipitis

P31867

without NaCl, pH 8.0, 30°C

762450

0.0094

NADPH

Rhizomucor pusillus

I7HD84

pH 7.0, 30°C

740777

0.0095

NADPH

Meyerozyma guilliermondii

-

pH 7.2, 25°C

763937

0.014

NADPH

Candida tropicalis

-

pH 7.0, D-xylose reductase 1

699278

0.018

NADPH

Candida tropicalis

-

pH 7.0, D-xylose reductase 2

699278

0.02

NADPH

Scheffersomyces shehatae

A0MTG4

pH 6.5, 35°C

696892

0.0277

NADPH

Scheffersomyces stipitis

P31867

with 150 mM NaCl, pH 8.0, 30°C

762450

0.028

NADPH

[Candida] intermedia

-

pH 7, 25°C

764945

0.061

NADPH

[Candida] intermedia

-

pH 7, 25°C

765140

2.3

NADPH

Yamadazyma tenuis

O74237

pH 7.0, 25°C, mutant enzyme D50A

696083

3.2

NADPH

Yamadazyma tenuis

O74237

pH 7.0, 25°C, wild-type enzyme

696083

7.6

NADPH

Saccharomyces cerevisiae

-

pH 7.0, 22°C, wild-type enzyme

701424

0.6

oenanthaldehyde

Saitozyma flava

-

pH 7, 25°C

764095

-

2.5

oenanthaldehyde

[Candida] intermedia

-

pH 7, 25°C

764095

-

334

xylitol

Yamadazyma tenuis

O74237

pH 7.0, 25°C, wild-type enzyme

696083

480

xylitol

[Candida] intermedia

-

pH 7, 25°C

764945

537

xylitol

Yamadazyma tenuis

O74237

pH 7.0, 25°C, mutant enzyme D50A

696083

additional information

additional information

Zymomonas mobilis subsp. mobilis

F2YCN5

Michaelis-Menten kinetics

721903

-

additional information

additional information

[Candida] boidinii

Q8X195

Michaelis-Menten kinetics

760768

-

additional information

additional information

[Candida] boidinii

-

Michaelis-Menten kinetics

760768

-

additional information

additional information

Thermomyces lanuginosus

A0A455KZK8

Michaelis-Menten kinetics

760720

-

additional information

additional information

[Candida] mogii

-

KM-value determined with cell extract

667284

-

additional information

additional information

Aspergillus niger

-

comparison of kinetic constants of NADPH dependent D-xylose reductases and other pentose reductases from different fungal species, overview

760777

-

additional information

additional information

Debaryomyces nepalensis

A0A0M4HL56

Michaelis-Menten and Lineweaver-Burk plots by linear and nonlinear regression fitting

761066

-

additional information

additional information

Debaryomyces nepalensis

A0A0M4HL56

thermodynamics, recombinant enzyme

761017

-