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Literature summary for 1.1.1.431 extracted from
- D-Xylose metabolism by Candida intermedia isolation and characterisation of two forms of aldose reductase with different coenzyme specificities (2000), J. Chromatogr. B Biomed. Sci. Appl., 737, 195-202 .
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.061 | - |
NADPH | pH 7, 25°C | [Candida] intermedia |  |
| 82 | - |
D-xylose | pH 7, 25°C | [Candida] intermedia | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 58000 | - |
gel filtration | [Candida] intermedia |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-xylose + NADPH + H+ | [Candida] intermedia | - |
xylitol + NADP+ | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| [Candida] intermedia | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| yeast cell extract is fractionated by biomimetic chromatography using the dye HE-3B (reactive Red 120) as pseudoaffinity ligand coupled to Sepharose CL-4B. In the second step, high-resolution anion-exchange chromatography using Mono Q yields purified isoform ALR2 in overall yield of 62% | [Candida] intermedia |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-xylose + NADPH + H+ | - |
[Candida] intermedia | xylitol + NADP+ | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 36000, SDS-PAGE, 2 * 35823, MALDI-TOF | [Candida] intermedia |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ALR1 | - |
[Candida] intermedia |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 14.6 | - |
NADPH | pH 7, 25°C | [Candida] intermedia | |
| 14.6 | - |
D-xylose | pH 7, 25°C | [Candida] intermedia | |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| [Candida] intermedia | isoelectric focusing | - |
4.4 |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | isoform ALR1 is strictly specific for NADPH, EC 1.1.1.431, whereas isoform ALR2 utilises NADH and NADPH with similar specificity constants, EC 1.1.1.307 | [Candida] intermedia |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.18 | - |
D-xylose | pH 7, 25°C | [Candida] intermedia | |
| 240 | - |
NADPH | pH 7, 25°C | [Candida] intermedia | |
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Release 2023.1 (January 2023)
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