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1.1.1.431: D-xylose reductase (NADPH)

This is an abbreviated version!
For detailed information about D-xylose reductase (NADPH), go to the full flat file.

Reaction

xylitol
+
NAD(P)+
=
D-xylose
 +
NAD(P)H
 +
H+

Synonyms

ALR1, Cb-XR, CbXR, CtXR, D-xylose reductase, D-xylose reductase 1, D-xylose reductase 2, D-xylose reductase 3, DnXR, GRE3, monospecific xylose reductase, More, msXR, NAD(P)H-dependent D-xylose reductase, NAD(P)H-dependent xylose reductase, NADPH dependent D-xylose reductase, NADPH-dependent D-xylose reductase II,III, NADPH-dependent xylose reductase, NADPH-preferring xylose reductase, NRRL3_10868, SsXR, Texr, TrxR, XR1, XR2, XR3, XRTL, XYL1, xylose reductase, xyr8, XyrA, XyrB

ECTree

     1 Oxidoreductases
         1.1 Acting on the CH-OH group of donors
             1.1.1 With NAD+ or NADP+ as acceptor
                1.1.1.431 D-xylose reductase (NADPH)

Temperature Stability

Temperature Stability on EC 1.1.1.431 - D-xylose reductase (NADPH)

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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30
purified recombinant wild-type enzyme has a half-life of 60 min, engineered enzyme Pir-XR of 120 min, and engineered enzyme XR-GPI of 120 min
40
purified recombinant wild-type enzyme has a half-life of 45 min, engineered enzyme Pir-XR of 100 min, and engineered enzyme XR-GPI of 100 min
40 - 60
the purified recombinant enzyme XRTL is extremely stable at 40°C and 50°C retaining 100% and 94.2% activity, respectively, after 120 min. The enzyme retains 24% activity at 60°C for 45 min, while the activity is completely lost at 70°C after 45 min. Half-lives (t1/2) of purified XRTL at 50°C, 55°C, and 60°C are 1386 min, 49.86 min, and 14.53 min, respectively
50
purified recombinant wild-type enzyme has a half-life of 30 min, engineered enzyme Pir-XR of 60 min, and engineered enzyme XR-GPI of 40 min
60
-
1 h, D-xylose reductase 1, D-xylose reductase 2, complete loss of activity
7
the purified recombinant enzyme is catalytically and structurally stable showing a melting temperature of 50°C
additional information
standard first-order enzyme deactivation kinetics