1.1.1.431: D-xylose reductase (NADPH)
This is an abbreviated version!
For detailed information about D-xylose reductase (NADPH), go to the full flat file.
Reaction
Synonyms
ALR1, Cb-XR, CbXR, CtXR, D-xylose reductase, D-xylose reductase 1, D-xylose reductase 2, D-xylose reductase 3, DnXR, GRE3, monospecific xylose reductase, More, msXR, NAD(P)H-dependent D-xylose reductase, NAD(P)H-dependent xylose reductase, NADPH dependent D-xylose reductase, NADPH-dependent D-xylose reductase II,III, NADPH-dependent xylose reductase, NADPH-preferring xylose reductase, NRRL3_10868, SsXR, Texr, TrxR, XR1, XR2, XR3, XRTL, XYL1, xylose reductase, xyr8, XyrA, XyrB
ECTree
1.1.1.431 D-xylose reductase (NADPH)Advanced search results
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results (Inhibitors
Inhibitors on EC 1.1.1.431 - D-xylose reductase (NADPH)
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INHIBITOR 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
IMAGE
DL-DTT
inhibits DnXR via mixed inhibition mechanism, a covalent adduct is formed between the nicotinamide ring and enzyme-bound DTT (NADP-DTT). In the DnXR complex, DTT is bound in the substrate-binding pocket in an orientation expected for a four-carbon open chain sugar alcohol, reminiscent of a product-bound form. The tertiary structure has an eight stranded barrel (beta1-beta8) is flanked by eight helices (alpha1-alpha8). A beta-hairpin caps the N-terminal end of the barrel while the C-terminal end contains the conserved active site groove. The extended C-terminal region containing two helices reaches over the active site with the terminal loop that is stabilized by interactions with residues from loop 4. Sequence comparisons
meso-erythritol
inhibits DnXR via mixed inhibition mechanism
NADP+
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competitive inhibitor against NADPH; for monospecific xylose reductase (msXR) and dual specific xylose reductase (dsXR) NADP+ behaves as a competitive inhibitor against NADPH. Competitive inhibition of is observed both at unsaturating and saturating concentrations of xylose
NADPH
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competitive inhibitor against NADP+; for monospecific xylose reductase (msXR) and dual specific xylose reductase (dsXR) NADPH behaves as a competitive inhibitor against NADP+. Competitive inhibition of is observed both at unsaturating and saturating concentrations of xylitol
additional information
wild-type and engineered enzymes are resistant to the addition of EDTA and are not much impaired by non-ionic detergents like Tweens or Triton X-100, but they are sensitive to SDS, PMSF and 2-mercaptoethanol
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