1.1.1.431: D-xylose reductase (NADPH)
This is an abbreviated version!
For detailed information about D-xylose reductase (NADPH), go to the full flat file.
Reaction
Synonyms
ALR1, Cb-XR, CbXR, CtXR, D-xylose reductase, D-xylose reductase 1, D-xylose reductase 2, D-xylose reductase 3, DnXR, GRE3, monospecific xylose reductase, More, msXR, NAD(P)H-dependent D-xylose reductase, NAD(P)H-dependent xylose reductase, NADPH dependent D-xylose reductase, NADPH-dependent D-xylose reductase II,III, NADPH-dependent xylose reductase, NADPH-preferring xylose reductase, NRRL3_10868, SsXR, Texr, TrxR, XR1, XR2, XR3, XRTL, XYL1, xylose reductase, xyr8, XyrA, XyrB
ECTree
1.1.1.431 D-xylose reductase (NADPH)Advanced search results
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results (Cloned
Cloned on EC 1.1.1.431 - D-xylose reductase (NADPH)
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CLONED (Commentary) 
ORGANISM
UNIPROT
LITERATURE
construction of Kluyveromyces marxianus strains expressing Pichia stipitis Psxyl1 genes leading to reversed cofactor specificity and ethanol and xylitol production
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expression in Escherichia coli
expression in Escherichia coli as a His6-tagged fusion protein
expression of the mutant xylulose reductase from Candida tenuis in Saccharomyces cerevisiae, co-expression with an engineered xylitol dehydrogenase, with altered cofactor specificity, from Galactocandida mastotermitis, the transformed strain shows up to 50% decreased glycerol yield without increase in ethanol during xylose fermentation, overview
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gene XRTL, DNA and amino acid sequence determination and analysis, recombinant enzyme expression in Pichia pastoris strain GS115 and Saccharomyces cerevisiae strain Y294 from the 19up-TEF1-XRTL-CYC-down-pAUR135 expression plasmid, subcloning in Escherichia coli strain JM109. The recombinant Saccharomyces cerevisiae strain with the XRTL gene shows 34% higher xylitol production compared to the control strain
gene Xyl1, phylogenetic analysis, recombinant expression of His6-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)-T1R
gene xyl1, recombinant expression in Escherichia coli strain BL21(DE3), coexpression with glucose dehydrogenase (GDH) gene of Bacillus cereus strain ATCC 14579
gene xyl1, recombinant expression of the enzyme in Synechococcus elongatus strain PCC7942, functional co-expression with a xylose transporter from Escherichia coli, Ec-XylE
gene xyr8, recombinant expression in Escherichia coli strain BL21(DE3), coexpression with glucose dehydrogenase (GDH) gene of Bacillus cereus strain ATCC 14579
gene xyrB or NRRL3_10868, phylogenetic and transcriptome analysis, phylogenetic tree, codon optimization of NRRL3_10868 and recombinant expression of His-tagged enzyme in Escherichia coli strain L21(DE3)pLysS
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gene ZMO0976, expression of His-tagged enzyme in Escherichia coli strain UT5600
overexpression of wild-type enzyme and mutant enzyme, with modified cofactor specificity, in Saccharomyces cerevisiae strain CEN.PK 113-7D under control of the constitutive TDH3 promoter, coexpression with xylitol dehydrogenase from Galactocandida mastotermitis, The strain harboring the xylose reductase double mutant shows 42% enhanced ethanol yield compared to the reference strain harboring wild-type xylose reductase during anaerobic bioreactor conversions of xylose. Likewise, the yields of xylitol and glycerol are decreased by 52% and 57% respectively in the xylose reductase mutant strain
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recombinant expression of His-tagged enzyme in Escherichia coli strain Rosetta
recombinant expression of the enzyme, constructs prepared for: (a) binding of xylose reductase to cell wall glucan through the fusion with the Pir4 cell wall protein (Pir-XR) expressed under regulation by the GAL1 promoter, (b) binding of xylose reductase to cell wall glucan through the Ccw12 GPI domain (XR-GPI) expressed under regulation by the GAL1 promoter, (c) secretion of xylose reductase into the medium (XR) expressed under regulation by the GAL1 promoter, (d) binding of xylose reductase to cell wall glucan through the Ccw12 GPI domain (XR-GPI) expressed under regulation by the PHO5 promoter. Relative activity of XR-GPI under PHO5 promoter at different concentrations of phosphate in growth media, overview
recombinant overexpression of strictly NADPH-dependent xylose reductase with mutated strictly NADP+-dependent xylitol dehydrogenase leading to increased bioethanol production and decreased xylitol accumulation in Saccharomyces cerevisiae strain D452-2 resulting in mutant strains SK-N1 to SK-N4
subcloned into the expression plasmid pET28a(+) and subsequently transformed into Escherichia coli BL21(DE3)pLysS cells for recombinant protein expression
