Literature summary for 1.1.1.431 extracted from

Chen, L.C.; Huang, S.C.; Chuankhayan, P.; Chen, C.D.; Huang, Y.C.; Jeyakanthan, J.; Pang, H.F.; Men, L.C.; Chen, Y.C.; Wang, Y.K.; Liu, M.Y.; Wu, T.K.; Chen, C.J.
Purification, crystallization and preliminary X-ray crystallographic analysis of xylose reductase from Candida tropicalis (2009), Acta Crystallogr. Sect. F, 65, 419-421.

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Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Candida tropicalis
subcloned into the expression plasmid pET28a(+) and subsequently transformed into Escherichia coli BL21(DE3)pLysS cells for recombinant protein expression	Candida tropicalis

Crystallization (Commentary)

Crystallization (Comment)	Organism
hanging-drop vapour diffusion method, to 2.91 A resolution. Unit cell belongs to space group P31 or P32, presence of four XR molecules in the asymmetric unit, with 68.0% solvent content	Candida tropicalis
hanging-drop vapour-diffusion method. X-ray diffraction data from xylose reductase crystals at 2.91 A resolution, the unit cell belongs to space group P3(1) or P3(2). Preliminary analysis indicates the presence of four xylose reductase molecules in the asymmetric unit, with 68.0% solvent content	Candida tropicalis

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
36600	-	x * 36600, calculated	Candida tropicalis
37000	-	x * 37000, SDS-PAGE	Candida tropicalis

Organism

Organism	UniProt	Comment	Textmining
Candida tropicalis	C1K8Y9	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Candida tropicalis
recombinant enzyme	Candida tropicalis

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
D-xylose + NADPH + H+	-	Candida tropicalis	xylitol + NADP+	-	?

Subunits

Subunits	Comment	Organism
?	x * 37000, SDS-PAGE	Candida tropicalis
?	x * 36600, calculated	Candida tropicalis

Synonyms

Synonyms	Comment	Organism
CtXR	-	Candida tropicalis
XYL1	-	Candida tropicalis

Cofactor

Cofactor	Comment	Organism	Structure
NADPH	-	Candida tropicalis

pI Value

Organism	Comment	pI Value Maximum	pI Value
Candida tropicalis	calculated	-	5.1

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Release 2023.1 (January 2023)