Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

2.7.7.1: nicotinamide-nucleotide adenylyltransferase

This is an abbreviated version!
For detailed information about nicotinamide-nucleotide adenylyltransferase, go to the full flat file.

Word Map on EC 2.7.7.1

Reaction

ATP
+
nicotinamide ribonucleotide
=
diphosphate
 +
NAD+

Synonyms

adenosine triphosphate-nicotinamide mononucleotide transadenylase, adenylyltransferase, nicotinamide mononucleotide, Arabidopsis thaliana nicotinate/nicotinamide mononucleotide adenyltransferase, AtNMNAT, ATP:NMN adenylyltransferase, diphosphopyridine nucleotide pyrophosphorylase, Drosophila NMNAT, GlNMNAT, hNMNAT, hNMNAT-1, hNMNAT-2, human nicotinamide mononucleotide adenylyl-transferase, m-nonN-Nmnat1, mononucleotide adenylyltransferase, More, NAD synthase, NAD+ pyrophosphorylase, nadD, NadM-Nudix, NadR, NaMNAT, nicotinamide 5'-mononucleotide adenylyltransferase, nicotinamide 5'-mononucleotide adenylyltransferase-2, nicotinamide 5-mononucleotide adenylyltransferase-2, nicotinamide adenine dinucleotide pyrophosphorylase, nicotinamide mononucleotide adenylyl-transferase, nicotinamide mononucleotide adenylyltransferase, nicotinamide mononucleotide adenylyltransferase 1, nicotinamide mononucleotide adenylyltransferase 2, nicotinamide mononucleotide adenylyltransferase type 1, nicotinamide mononucleotide adenylyltransferase1, nicotinamide mononucleotide adenylyltransferase2, nicotinamide/nicotinate mononucleotide adenylyltransferase, nicotinamide/nicotinic acid mononucleotide adenylyltransferase, nicotinamide/nicotinic acid mononucleotide adenylyltransferase 1, nicotinate/nicotinamide mononucleotide adenyltransferase, NMN adenylyl transferase 1, NMN adenylyltransferase, NMN adenylyltransferase/ADP-ribose pyrophosphatase, NMN AT, NMN-adenylyltransferase, NMN/NaMN adenylyltransferase, NMN/NaMN adenylyltransferase 2, NMN/NaMN adenylyltransferase 3, NMNAT, NMNAT-2, NMNAT1, NMNAT2, NMNAT3, NMNATase, non-nuclear-localized-Nmnat1, PF3D7_1327600, PNAT, Pof1, pyridine nucleotide adenylyltransferase, Tc00.1047053507047.170, TK0067, TTHA1780, yNMNAT-1, yNMNAT-2

ECTree

     2 Transferases
         2.7 Transferring phosphorus-containing groups
             2.7.7 Nucleotidyltransferases
                2.7.7.1 nicotinamide-nucleotide adenylyltransferase

Cloned

Cloned on EC 2.7.7.1 - nicotinamide-nucleotide adenylyltransferase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli Rosetta (DE3)pLysS cells
expression in Escherichia coli
expression in Escherichia coli BL21
expression in Escherichia coli BL21 with a 6 x His-tag
expression in Escherichia coli, NMNAT-2 with a 6 x His-tag
-
gene LBRM_17_1470 or lbnmnat, DNA and amino acid sequence determination and analysis, sequence comparisons, primary structure analysis reveals a Leishmania-specific amino-terminal insertion in NMNAT. The deletion of this insertion is negatively correlates with in vitro enzymatic activity. Recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3)
gene MTH_150, recombinant overexpression of N-terminally His6-tagged wild-type and mutant enzymes in Escherichia coli strain BL21 Gold (DE3)
gene NMNAT1, recombinant expression in H-1299 and U2-O2 cells, recombinant co-expression of His6-tagged NMNAT1 and GST-tagged NML in Escherichia coli, co-expression of FLAG-tagged nucleomethylin and Myc-tagged NMNAT1 in H-1299 cells
gene Nmnat1, recombinant expression of mouse Nmnat1 non-nuclear-localized gain-of-function mutant gene (m-nonN-Nmnat1) in Caenorhabditis elegans. The transgenic enzyme provides protection from both hypoxia-induced animal death and taxol-induced axonal pathology and significantly lengthens the nematode's lifespan. Loss of function in two genes, haf-1 and dve-1, encoding mitochondrial unfolded protein response (mitoUPR) factors are identified as suppressors. M-nonN-Nmnat1 induces a transcriptional reporter of the mitoUPR gene hsp-6 and provides protection from the mitochondrial proteostasis toxin ethidium bromide. M-nonN-Nmnat1 is also protective against axonal degeneration in Caenorhabditis elegans induced by the chemotherapy drug taxol. Taxol markedly reduces basal expression of a mitoUPR reporter, the expression is restored by m-nonN-Nmnat1
gene NMNAT2, semi-quantitative and real-time PCR enzyme expression analysis
gene NMNAT2, transfection of hNMNAT2 results in a six- and threefold cytoplasmic overexpression in Caco-2 and HT-29 cell lines, respectively, correlating with Tiazofurin-induced enhanced cell killing
gene Pof1 or YCL047C, DNA and amino acid sequence determination and analysis, sequence comparisons, construction of the POF1-URA3 episomal plasmid (pRS316-POF1) derived from pADH1-POF1, which is constructed in the integrative pPP81 (LEU2) vector, recombinant expression of His-tagged enzyme from plasmid pET28-POF1 in Escherichia coli strain Rosetta (DE3), quantitative PCR expression analysis
gene TcCLB.507047.170 or Tc00.1047053507047.170, DNA and amino acid sequence determination and analysis from genomic DNA, recombinant expression of N-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3)
genes glnmnat-a and glnmnat-b, encoded on chromosome 3 and 5, respectively, DNA and amino acid sequence determination and analysis, phylogenetic analysis of GlNMNAT isoenzymes, sequence comparisons to human isozymes, recombinant expression of His-tagged glnmnat-a gene product in Escherichia coli strain BL21
His-tagged isozymes NMNAT1, NMNAT2, and NMNAT3, expression in Escherichia coli strain BL21(DE3)
overexpressed in Escherichia coli BL21(DE3)
-
overexpression in Escherichia coli of PNAT-3 with a His-tag
-
overexpression in HeLa and HEK293 cells as GFP-fusion protein
-
transgenic mice overexpressing NMNAT1 and the mutant WldS(W258A) are generated
transgenic mice overexpressing NMNAT3 are generated