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Literature summary for 2.7.7.1 extracted from
- Expression, localization, and biochemical characterization of nicotinamide mononucleotide adenylyltransferase 2 (2010), J. Biol. Chem., 285, 40387-40396.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli Rosetta (DE3)pLysS cells | Mus musculus |
| expressed in Escherichia coli Rosetta (DE3)pLysS cells | Homo sapiens |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| C164S/C165S | the mutant protein is not palmitoylated (soluble), appears diffuse in the cytoplasm and does not colocalize with GM130. However, the mutant is fully active and shows even higher kcat values compared to the wild type enzyme | Mus musculus |
| C164S/C165S | the mutant protein is not palmitoylated (soluble), appears diffuse in the cytoplasm and does not colocalize with GM130. However, the mutant is fully active and shows even higher kcat values compared to the wild type enzyme | Homo sapiens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00295 | - |
nicotinamide ribonucleotide | mutant enzyme C164S/C165S, in 50 mM HEPES-NaOH, pH 7.5, temperature not specified in the publication | Mus musculus |  |
| 0.00691 | - |
nicotinamide ribonucleotide | wild type enzyme, in 50 mM HEPES-NaOH, pH 7.5, temperature not specified in the publication | Mus musculus | |
| 0.08425 | - |
ATP | wild type enzyme, in 50 mM HEPES-NaOH, pH 7.5, temperature not specified in the publication | Mus musculus | |
| 0.113 | - |
ATP | mutant enzyme C164S/C165S, in 50 mM HEPES-NaOH, pH 7.5, temperature not specified in the publication | Mus musculus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| endosome | in HeLa cells, in addition to Golgi, isoform Nmnat2 localizes to Rab7-containing late endosomes | Homo sapiens | 5768 | - |
| Golgi apparatus | isoform Nmnat2 is enriched in numerous membrane compartments including synaptic terminals | Mus musculus | 5794 | - |
| Golgi apparatus | isoform Nmnat2 is enriched in numerous membrane compartments including synaptic terminals | Homo sapiens | 5794 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 34000 | - |
x * 34000, SDS-PAGE | Mus musculus |
| 34000 | - |
x * 34000, SDS-PAGE | Homo sapiens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | Q9BZQ4 | isoform NMNAT2 | - |
| Mus musculus | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| palmitoylation | isoform Nmnat2 is palmitoylated in vitro (at Cys164 and Cys165) and this modification is required for membrane association | Mus musculus |
| palmitoylation | isoform Nmnat2 is palmitoylated in vitro (at Cys164 and Cys165) and this modification is required for membrane association | Homo sapiens |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-NTA agarose bead chromatography | Mus musculus |
| Ni-NTA agarose bead chromatography | Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| brain | Nmnat2 transcript is expressed predominately in the brain | Mus musculus | - |
| brain | Nmnat2 transcript is expressed predominately in the brain | Homo sapiens | - |
| HeLa cell | - |
Homo sapiens | - |
| additional information | isoform Nmat2 is not detected in heart, spleen, lung, kidney, liver and skeletal muscle | Mus musculus | - |
| additional information | isoform Nmat2 is not detected in HEK-293T cells, NIH-3T3 cells, U-343 cells, U-87 cells, and primary glia cells | Homo sapiens | - |
| Neuro-2a cell | - |
Homo sapiens | - |
| neuron | primary cortical neuron | Mus musculus | - |
| neuron | primary cortical neuron | Homo sapiens | - |
| SH-SY5Y cell | differentiated | Mus musculus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + nicotinamide ribonucleotide | - |
Mus musculus | diphosphate + NAD+ | - |
? | |
| ATP + nicotinamide ribonucleotide | - |
Homo sapiens | diphosphate + NAD+ | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 34000, SDS-PAGE | Mus musculus |
| ? | x * 34000, SDS-PAGE | Homo sapiens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| nicotinamide mononucleotide adenylyltransferase 2 | - |
Mus musculus |
| nicotinamide mononucleotide adenylyltransferase 2 | - |
Homo sapiens |
| NMNAT2 | - |
Mus musculus |
| NMNAT2 | - |
Homo sapiens |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.654 | - |
nicotinamide ribonucleotide | wild type enzyme, in 50 mM HEPES-NaOH, pH 7.5, temperature not specified in the publication | Mus musculus | |
| 7.21 | - |
nicotinamide ribonucleotide | mutant enzyme C164S/C165S, in 50 mM HEPES-NaOH, pH 7.5, temperature not specified in the publication | Mus musculus | |
Expression
| Organism | Comment | Expression |
|---|---|---|
| Mus musculus | isoform Nmnat2 protein levels increase dramatically upon differentiation of SH-SY5Y cells | up |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | overexpression of isoform Nmnat2 is toxic to primary neurons resulting in pervasive cell death | Mus musculus |
| physiological function | overexpression of isoform Nmnat2 is toxic to primary neurons resulting in pervasive cell death | Homo sapiens |
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