Application | Comment | Organism |
---|---|---|
pharmacology | the amino-terminal insertion of Leishmania NMNATs as a promising pharmacological target for the development of specific control strategies | Leishmania braziliensis |
Cloned (Comment) | Organism |
---|---|
gene LBRM_17_1470 or lbnmnat, DNA and amino acid sequence determination and analysis, sequence comparisons, primary structure analysis reveals a Leishmania-specific amino-terminal insertion in NMNAT. The deletion of this insertion is negatively correlates with in vitro enzymatic activity. Recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3) | Leishmania braziliensis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.13 | - |
ATP | pH 7.4, 37°C, recombinant enzyme, kinetics following Hanes-Woolf method | Leishmania braziliensis | |
0.15 | - |
ATP | pH 7.4, 37°C, recombinant enzyme, kinetics following Eadie-Hofstee method | Leishmania braziliensis | |
0.189 | - |
ATP | pH 7.4, 37°C, recombinant enzyme, kinetics following Lineweaver-Burk method | Leishmania braziliensis | |
0.755 | - |
nicotinamide ribonucleotide | pH 7.4, 37°C, recombinant enzyme, kinetics following Hanes-Woolf method | Leishmania braziliensis | |
0.926 | - |
nicotinamide ribonucleotide | pH 7.4, 37°C, recombinant enzyme, kinetics following Eadie-Hofstee method | Leishmania braziliensis | |
0.985 | - |
nicotinamide ribonucleotide | pH 7.4, 37°C, recombinant enzyme, kinetics following Lineweaver-Burk method | Leishmania braziliensis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required, best divalent cation | Leishmania braziliensis | |
Mn2+ | 42% of the activity with Mg2+ | Leishmania braziliensis | |
additional information | no activity with Ag+, Ca2+, or Cu2+ | Leishmania braziliensis | |
Ni2+ | 87% of the activity with Mg2+ | Leishmania braziliensis | |
Zn2+ | 9% of the activity with Mg2+ | Leishmania braziliensis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + nicotinamide ribonucleotide | Leishmania braziliensis | - |
diphosphate + NAD+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Leishmania braziliensis | A4H990 | gene LBRM_17_1470 or lbnmnat | - |
Purification (Comment) | Organism |
---|---|
recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration | Leishmania braziliensis |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
promastigote | - |
Leishmania braziliensis | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
28.22 | - |
purified recombinant His-tagged enzyme, pH 7.4, 37°C | Leishmania braziliensis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + nicotinamide ribonucleotide | - |
Leishmania braziliensis | diphosphate + NAD+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
nicotinamide mononucleotide adenylyltransferase | - |
Leishmania braziliensis |
NMNAT | - |
Leishmania braziliensis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Leishmania braziliensis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.718 | - |
nicotinamide ribonucleotide | pH 7.4, 37°C, recombinant enzyme, kinetics following Hanes-Woolf method | Leishmania braziliensis | |
0.73 | - |
nicotinamide ribonucleotide | pH 7.4, 37°C, recombinant enzyme, kinetics following Eadie-Hofstee method | Leishmania braziliensis | |
0.76 | - |
nicotinamide ribonucleotide | pH 7.4, 37°C, recombinant enzyme, kinetics following Lineweaver-Burk method | Leishmania braziliensis | |
0.969 | - |
ATP | pH 7.4, 37°C, recombinant enzyme, kinetics following Hanes-Woolf method | Leishmania braziliensis | |
0.984 | - |
ATP | pH 7.4, 37°C, recombinant enzyme, kinetics following Eadie-Hofstee method | Leishmania braziliensis | |
1.174 | - |
ATP | pH 7.4, 37°C, recombinant enzyme, kinetics following Lineweaver-Burk method | Leishmania braziliensis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Leishmania braziliensis |
General Information | Comment | Organism |
---|---|---|
additional information | structural homology modeling using human NMNAT3, PDB ID 1NUP, B chain, as a template, overview | Leishmania braziliensis |
physiological function | nicotinamide mononucleotide adenylyltransferase catalyzes the central step in nicotinamide adenine dinucleotide (NAD+) biosynthesis, making it essential for survival | Leishmania braziliensis |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.771 | - |
nicotinamide ribonucleotide | pH 7.4, 37°C, recombinant enzyme, kinetics following Lineweaver-Burk method | Leishmania braziliensis | |
0.789 | - |
nicotinamide ribonucleotide | pH 7.4, 37°C, recombinant enzyme, kinetics following Eadie-Hofstee method | Leishmania braziliensis | |
0.951 | - |
nicotinamide ribonucleotide | pH 7.4, 37°C, recombinant enzyme, kinetics following Hanes-Woolf method | Leishmania braziliensis | |
6.218 | - |
ATP | pH 7.4, 37°C, recombinant enzyme, kinetics following Lineweaver-Burk method | Leishmania braziliensis | |
6.549 | - |
ATP | pH 7.4, 37°C, recombinant enzyme, kinetics following Eadie-Hofstee method | Leishmania braziliensis | |
7.481 | - |
ATP | pH 7.4, 37°C, recombinant enzyme, kinetics following Hanes-Woolf method | Leishmania braziliensis |