Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Mus musculus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0252 | - |
nicotinamide ribonucleotide | isoform NMNAT1, in 30 mM HEPES/KOH, pH 7.5, at 37°C | Mus musculus | |
0.0335 | - |
ATP | isoform NMNAT1, in 30 mM HEPES/KOH, pH 7.5, at 37°C | Mus musculus | |
0.0385 | - |
nicotinamide ribonucleotide | isoform NMNAT2, in 30 mM HEPES/KOH, pH 7.5, at 37°C | Mus musculus | |
0.039 | - |
ATP | isoform NMNAT3, in 30 mM HEPES/KOH, pH 7.5, at 37°C | Mus musculus | |
0.082 | - |
ATP | isoform NMNAT2, in 30 mM HEPES/KOH, pH 7.5, at 37°C | Mus musculus | |
0.1176 | - |
nicotinamide ribonucleotide | isoform NMNAT3, in 30 mM HEPES/KOH, pH 7.5, at 37°C | Mus musculus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | isoform NMNAT3 is predominantly activated by Co2+ ions (5 mM) | Mus musculus | |
Mg2+ | isoform NMNAT2 is predominantly activated by Mg2+ ions (0.5 mM) | Mus musculus | |
Zn2+ | isoform NMNAT1 is predominantly activated by Zn2+ ions (0.05 mM) | Mus musculus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | Q5HZI3 | isoform NMNAT2 | - |
Mus musculus | Q99JR6 | isoform NMNAT3 | - |
Mus musculus | Q9EPA7 | isoform NMNAT1 | - |
Purification (Comment) | Organism |
---|---|
Ni-NTA affinity chromatography | Mus musculus |
Talon affinity column chromatography | Mus musculus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | - |
Mus musculus | - |
liver | - |
Mus musculus | - |
Storage Stability | Organism |
---|---|
-20°C, at pH 7.5 in buffer containing both glycerol and TCEP, 6 months, about 20% loss of activity | Mus musculus |
-20°C, at pH 7.5 in buffer containing both glycerol and TCEP, 6 months, about 55% loss of activity | Mus musculus |
-20°C, at pH 7.5 in buffer containing both glycerol and TCEP, 6 months, about 60% loss of activity | Mus musculus |
4°C, at pH 7.5 in buffer containing both glycerol and TCEP, 6 months, about 55% loss of activity | Mus musculus |
4°C, at pH 7.5 in buffer containing both glycerol and TCEP, 6 months, about 60% loss of activity | Mus musculus |
4°C, at pH 7.5 in buffer containing both glycerol and TCEP, 6 months, about 90% loss of activity | Mus musculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + nicotinamide ribonucleotide | - |
Mus musculus | diphosphate + NAD+ | - |
r | |
ATP + nicotinic acid mononucleotide | - |
Mus musculus | diphosphate + deamido-NAD+ | - |
r | |
GTP + nicotinamide ribonucleotide | - |
Mus musculus | ? | - |
? | |
ITP + nicotinamide ribonucleotide | - |
Mus musculus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
nicotinamide mononucleotide adenylyltransferase | - |
Mus musculus |
NMNAT1 | isoform | Mus musculus |
NMNAT2 | isoform | Mus musculus |
NMNAT3 | isoform | Mus musculus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.74 | - |
ATP | isoform NMNAT3, in 30 mM HEPES/KOH, pH 7.5, at 37°C | Mus musculus | |
0.75 | - |
nicotinamide ribonucleotide | isoform NMNAT3, in 30 mM HEPES/KOH, pH 7.5, at 37°C | Mus musculus | |
2.33 | - |
ATP | isoform NMNAT2, in 30 mM HEPES/KOH, pH 7.5, at 37°C | Mus musculus | |
2.87 | - |
nicotinamide ribonucleotide | isoform NMNAT2, in 30 mM HEPES/KOH, pH 7.5, at 37°C | Mus musculus | |
15 | - |
ATP | isoform NMNAT1, in 30 mM HEPES/KOH, pH 7.5, at 37°C | Mus musculus | |
15.5 | - |
nicotinamide ribonucleotide | isoform NMNAT1, in 30 mM HEPES/KOH, pH 7.5, at 37°C | Mus musculus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
10 | - |
nicotinamide ribonucleotide | isoform NMNAT3, in 30 mM HEPES/KOH, pH 7.5, at 37°C | Mus musculus | |
20 | - |
ATP | isoform NMNAT3, in 30 mM HEPES/KOH, pH 7.5, at 37°C | Mus musculus | |
30 | - |
ATP | isoform NMNAT2, in 30 mM HEPES/KOH, pH 7.5, at 37°C | Mus musculus | |
70 | - |
nicotinamide ribonucleotide | isoform NMNAT2, in 30 mM HEPES/KOH, pH 7.5, at 37°C | Mus musculus | |
450 | - |
ATP | isoform NMNAT1, in 30 mM HEPES/KOH, pH 7.5, at 37°C | Mus musculus | |
610 | - |
nicotinamide ribonucleotide | isoform NMNAT1, in 30 mM HEPES/KOH, pH 7.5, at 37°C | Mus musculus |