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2.4.2.9: uracil phosphoribosyltransferase

This is an abbreviated version!
For detailed information about uracil phosphoribosyltransferase, go to the full flat file.

Word Map on EC 2.4.2.9

Reaction

UMP
+
diphosphate
=
Uracil
 +
5-phospho-alpha-D-ribose 1-diphosphate

Synonyms

AFR052C, AFR052Cp, AGOS_AFR052C, AgUPRT, CD-UPRT, cytosine deaminase-uracil phosphoribosyltransferase, cytosine deaminase/uracil phosphoribosyltransferase, More, MtUPRT, phosphoribosyltransferase, uracil, Rv3309c, TtUPRT, TtUPRT3, UK/UPRT1, UMP pyrophosphorylase, UMP:pyrophosphate phosphoribosyltransferase, UPP, UPRT, UPRTase, uracil phosphoribosyl transferase, uridine 5'-phosphate pyrophosphorylase, uridine kinase-like protein (uracil phosphoribosyltransferase), uridine kinase/uracil phosphoribosyltransferase 1, uridine monophosphate pyrophosphorylase, uridylate pyrophosphorylase, uridylic pyrophosphorylase

ECTree

     2 Transferases
         2.4 Glycosyltransferases
             2.4.2 Pentosyltransferases
                2.4.2.9 uracil phosphoribosyltransferase

Cloned

Cloned on EC 2.4.2.9 - uracil phosphoribosyltransferase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
cloning from a fetal brain cDNA library, DNA and amino acid sequence determination, subcloning in Escherichia coli strain DH5alpha, expression of His6-tagged enzyme in Escherichia coli strain M15, phylogenetic analysis, expressionin AD293 cells, tissue and subcellular expression pattern
co-expression of gene CodA, encoding cytosine deaminase, and gene upp, encoding uracil phosphoribosyl transferase, renders HeLa cells sensitive to 5-fluorocytosine, converted to toxic 5-fluorouracil by cytosine deaminase, and enhanced a bystander effect mediated by a gap junction mechanism, 1% cytosine deaminase+-UPP+ cells are able to kill 40% of the cell population if the cells are communicating
-
coexpression of the uracil phosphoribosyltransferase gene with a chimeric human nerve growth factor receptor/yeast cytosine deaminase fusion gene, using a single retroviral vector, augments cytotoxicity of transduced human T cells, CEM cells, exposed to 5-fluorocytosine
-
cytosine deaminase-UPRT-red fluorescence protein fusion proteins are expressed in Rattus norvegicus R-3327-AT cells
DNA and amino acid sequence determination and analysis of the natural chimeric gene UK/UPRT1 encoding uridine kinase, UK, and uracil phosphoribosyltransferase, UPRT, expressionin Escherichia coli, transient expression as C-terminally GFP-tagged enzyme in Arabidopsis thaliana mesophyll protoplasts, 5-FU sensitivity of the Escherichia coli upp mutants is influenced by the expression of AtUK/UPRT1, as well as the 5-FU and 5-FD sensitivity of Escherichia coli upp-udk mutants, overview
DNA and amino acid sequence determination and analysis, overexpression in enzyme-deficient Escherichia coli
DNA sequence determination and analysis, gene upp belongs to purMN operon, complementation of deficient Escherichia coli strain
-
DNA sequence determination, functional overexpression in Escherichia coli BL21(DE3)plyS
expressed in A2780 cells, A2780/Taxol cells, Skov3 cells, and Skov3/Taxol cells
expressed in AsPC-1, BxPC3, CaPan-1, MIA, PaCa-2, Panc-1, and HT-29 cell lines
-
expressed in BHK-21 cells and HAT-29 cells
-
expressed in Escherichia coli BL21(DE3) cells
-
expressed in Escherichia coli BL21Star (DE3) One Shot cells
-
expressed in Escherichia coli strain JM109
expressed in Escherichia coli strain M15 and in AD-293 cells
expressed in Escherichia coli Tuner (DE3) cells
expressed in HaP-T1 cells
Syrian hamster adenovirus
-
expressed in HeLa cells
-
expressed in LNCaP cells
-
expression in Escherichia coli
-
expression in human adipose tissue-derived mesenchymal stem cells
-
expression of the fusion gene CDUPRT, combining cytosine deaminase combined with uracil phosphoribosyl transferase, in RM1 cancer cells in a gene therapeutic approach, overview
-
functional overexpression in Escherichia coli JM109
gene AGOS_AFR052C, sequence comparisons, phylogenetic tree, functional recombinant expression of the enzyme with an N-terminal fusion to TrxA as a soluble enzyme in Escherichia coli
gene UPP, DNA and amino acid sequence determination and analysis, gene structure, sequence comparison, and phylogenetic analysis, Comparison to uracil kinase-like proteins possessing UPRT domains. The single nuclear gene encoding UPP targeted to plastids is responsible for almost all UPRT activity in Arabidopsis thaliana
gene upp, recombinant expression, the basal level of MtUPRT expression is independent of either growth medium used, addition of bases, or oxygen presence/absence
gene upp, recombinant overexpression of 35S:FCY-UPP and pMYB60:FCY-UPP constructs in Arabdopsis thaliana ecotype Col-0, which affects the effect of 5-fluorouracil (5-FC) on the plants compared to wild-type plants, analysis of the tissue specificity of the FCY-UPP tandem, overview. The enzyme tandem is expressed in the xylem pole pericycle cells from which lateral roots are being formed. Expressing FCYUPP in the lateral root cap and epidermis using the J0951 enhancer trap line results in a strong reduction of primary root growth. Similarly, when FCY-UPP proteins are expressed in the guard cells using the pMYB60 promoter, no reduction in total leaf area is observed. These observations suggest that both the FCY-UPP tandem protein and the products of 5-FC incorporation remain in the cells where the construct is expressed, at least on a short time-scale. A tagged version of FCY-UPP fused to the GFP reporter is expressed under the epidermis-specific promoter of WEREWOLF
gene upp, upp gene is part of the lp_2376-glyA-upp operon, upp expression depends on the upp-pyrP gene cluster, pyrP encodes a high affinity uracil transporter, which is expressed and regulated independently of upp, overview
overexpressing from plasmid in Escherichia coli strain NF1815
-
overexpression of wild-type and mutant P131D in Escherichia coli
-
recombinant expression of N-terminal His6-tagged TtUPRT
wild-type and mutant C128V