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Literature summary for 2.4.2.9 extracted from
- Recombinant uracil phosphoribosyltransferase from the thermophile Bacillus caldolyticus: expression, purification, and partial characterization (1997), Protein Expr. Purif., 10, 356-364.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| DNA and amino acid sequence determination and analysis, overexpression in enzyme-deficient Escherichia coli | [Bacillus] caldolyticus |
General Stability
| General Stability | Organism |
|---|---|
| bovine serum albumin at 2 mg/ml stabilizes | [Bacillus] caldolyticus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.002 | - |
Uracil | - |
[Bacillus] caldolyticus |  |
| 0.05 | - |
5-phospho-alpha-D-ribose 1-diphosphate | - |
[Bacillus] caldolyticus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 46000 | - |
gel filtration | [Bacillus] caldolyticus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus subtilis | - |
- |
- |
| Bacillus subtilis 168 | - |
- |
- |
| [Bacillus] caldolyticus | P70881 | gene upp | - |
| [Bacillus] caldolyticus | P70881 | strain DSM 405 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant from enzyme-deficient Escherichia coli | [Bacillus] caldolyticus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.07 | - |
37°C | Bacillus subtilis |
| 0.09 | - |
60°C | [Bacillus] caldolyticus |
| 19.2 | - |
purified recombinant enzyme, 37°C | [Bacillus] caldolyticus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 0°C, enzyme concentration 0.001 mg/ml, 50% loss of activity in 1 h | [Bacillus] caldolyticus |
| 4°C, concentrated enzyme is stable for several months, on dilution occurs rapid loss of activity | [Bacillus] caldolyticus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| uracil + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Bacillus subtilis | UMP + diphosphate | - |
? | |
| uracil + 5-phospho-alpha-D-ribose 1-diphosphate | - |
[Bacillus] caldolyticus | UMP + diphosphate | - |
? | |
| uracil + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Bacillus subtilis 168 | UMP + diphosphate | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
recombinant enzyme | [Bacillus] caldolyticus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
10 min, 90% remaining activity | Bacillus subtilis |
| 55 | - |
10 min, 20% remaining activity | Bacillus subtilis |
| 60 | - |
10 min, inactivation | Bacillus subtilis |
| 70 | - |
10 min, 70% remaining activity | [Bacillus] caldolyticus |
| 75 | - |
10 min, 5% remaining activity | [Bacillus] caldolyticus |
| 80 | - |
10 min, inactivation | [Bacillus] caldolyticus |
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