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Literature summary for 2.4.2.9 extracted from

  • Villela, A.D.; Ducati, R.G.; Rosado, L.A.; Bloch, C.J.; Prates, M.V.; Goncalves, D.C.; Ramos, C.H.; Basso, L.A.; Santos, D.S.
    Biochemical characterization of uracil phosphoribosyltransferase from Mycobacterium tuberculosis (2013), PLoS ONE, 8, e56445.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.4.2.9

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Mycobacterium tuberculosis

Inhibitors

Inhibitors Comment Organism Structure
additional information not significantly activated or inhibited by ATP, UTP, and CTP Mycobacterium tuberculosis
UMP substrate inhibition at 0.1 mM UMP Mycobacterium tuberculosis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0124
-
 Uracil at pH 7.5 and 25°C Mycobacterium tuberculosis

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Mycobacterium tuberculosis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
21289
-
 4 * 21289, calculated from amino acid sequence Mycobacterium tuberculosis
22000
-
 4 * 22000, SDS-PAGE Mycobacterium tuberculosis
109700
-
 gel filtration Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
uracil + 5-phospho-alpha-D-ribose 1-diphosphate Mycobacterium tuberculosis the enzyme is specific for uracil UMP + diphosphate
-
 r
uracil + 5-phospho-alpha-D-ribose 1-diphosphate Mycobacterium tuberculosis H37Rv the enzyme is specific for uracil UMP + diphosphate
-
 r

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis
-
-
-
Mycobacterium tuberculosis H37Rv
-
-
-

Purification (Commentary)

Purification (Comment) Organism
DEAE Sepharose column chromatography, Mono Q column chromatography, and Sephacryl S-300 gel filtration Mycobacterium tuberculosis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.76
-
 crude extract, at pH 7.5 and 25°C Mycobacterium tuberculosis
1.56
-
 after 2.1fold purification, at pH 7.5 and 25°C Mycobacterium tuberculosis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
uracil + 5-phospho-alpha-D-ribose 1-diphosphate the enzyme is specific for uracil Mycobacterium tuberculosis UMP + diphosphate
-
 r
uracil + 5-phospho-alpha-D-ribose 1-diphosphate the enzyme is specific for uracil Mycobacterium tuberculosis H37Rv UMP + diphosphate
-
 r

Subunits

Subunits Comment Organism
homotetramer 4 * 22000, SDS-PAGE Mycobacterium tuberculosis
homotetramer 4 * 21289, calculated from amino acid sequence Mycobacterium tuberculosis

Synonyms

Synonyms Comment Organism
UPRT
-
 Mycobacterium tuberculosis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.65
-
 Uracil at pH 7.5 and 25°C Mycobacterium tuberculosis