Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21Star (DE3) One Shot cells | Leishmania donovani |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4-thiouracil | substrate inhibition at high concentrations | Leishmania donovani | |
5-fluorouracil | substrate inhibition at high concentrations | Leishmania donovani | |
EDTA | - |
Leishmania donovani | |
Uracil | substrate inhibition at high concentrations | Leishmania donovani |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0064 | - |
5-fluorouracil | at pH 7.5 and 25°C | Leishmania donovani | |
0.0071 | - |
4-thiouracil | at pH 7.5 and 25°C | Leishmania donovani | |
0.0204 | - |
Uracil | at pH 7.5 and 25°C | Leishmania donovani | |
0.0993 | - |
5-phospho-alpha-D-ribose 1-diphosphate | with uracil as cosubstrate, at pH 7.5 and 25°C | Leishmania donovani |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | can replace Mg2+ | Leishmania donovani | |
Mg2+ | maximum activity in the presence of 5 mM Mg2+ | Leishmania donovani | |
Mn2+ | can replace Mg2+ | Leishmania donovani | |
additional information | not stimulated by Ba2+, Ca2+, Ni2+, and Zn2+ | Leishmania donovani |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
27000 | - |
4 * 27000, SDS-PAGE | Leishmania donovani |
100000 | - |
gel filtration | Leishmania donovani |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
uracil + 5-phospho-alpha-D-ribose 1-diphosphate | Leishmania donovani | - |
UMP + diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Leishmania donovani | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography | Leishmania donovani |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-thiouracil + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Leishmania donovani | 4-thio-UMP + diphosphate | - |
? | |
5-fluorouracil + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Leishmania donovani | 5-fluoro-UMP + diphosphate | - |
? | |
uracil + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Leishmania donovani | UMP + diphosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | 4 * 27000, SDS-PAGE | Leishmania donovani |
Synonyms | Comment | Organism |
---|---|---|
UPRT | - |
Leishmania donovani |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Leishmania donovani |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.5 | 8.5 | more than 90% activity between 7.5 and 8.5 with sharp drops in activity 0.5 pH units outside this range | Leishmania donovani |