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Literature summary for 2.4.2.9 extracted from

  • Ghode, P.; Jobichen, C.; Ramachandran, S.; Bifani, P.; Sivaraman, J.
    Structural basis of mapping the spontaneous mutations with 5-flurouracil in uracil phosphoribosyltransferase from Mycobacterium tuberculosis (2015), Biochem. Biophys. Res. Commun., 467, 577-582.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Tuner (DE3) cells Mycobacterium tuberculosis

Crystallization (Commentary)

Crystallization (Comment) Organism
sitting drop vapor diffusion method, using 0.1 M sodium acetate trihydrate pH 4.6, 1.5 M sodium formate with a 2:1 reservoir-to-protein solution ratio at 25°C Mycobacterium tuberculosis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
25000
-
4 * 25000, SDS-PAGE Mycobacterium tuberculosis
120000
-
gel filtration Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
uracil + 5-phospho-alpha-D-ribose 1-diphosphate Mycobacterium tuberculosis
-
UMP + diphosphate
-
r
uracil + 5-phospho-alpha-D-ribose 1-diphosphate Mycobacterium tuberculosis H37Rv
-
UMP + diphosphate
-
r

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis P9WFF3
-
-
Mycobacterium tuberculosis H37Rv P9WFF3
-
-

Purification (Commentary)

Purification (Comment) Organism
MonoQ column chromatography and Superdex 200 gel filtration Mycobacterium tuberculosis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
uracil + 5-phospho-alpha-D-ribose 1-diphosphate
-
Mycobacterium tuberculosis UMP + diphosphate
-
r
uracil + 5-phospho-alpha-D-ribose 1-diphosphate
-
Mycobacterium tuberculosis H37Rv UMP + diphosphate
-
r

Subunits

Subunits Comment Organism
homotetramer 4 * 25000, SDS-PAGE Mycobacterium tuberculosis

Synonyms

Synonyms Comment Organism
UPRT
-
Mycobacterium tuberculosis