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Information on EC 1.1.1.49 - glucose-6-phosphate dehydrogenase (NADP+)
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1.1.1.49 glucose-6-phosphate dehydrogenase (NADP+)IUBMB Comments
The enzyme catalyses a step of the pentose phosphate pathway. The enzyme is specific for NADP+. cf. EC 1.1.1.363, glucose-6-phosphate dehydrogenase [NAD(P)+] and EC 1.1.1.388, glucose-6-phosphate dehydrogenase (NAD+).
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Word Map
- 1.1.1.49
- erythrocyte
- pentose
-
hemolytic
- catalase
- malaria
- 6-phosphogluconate
- anemia
- dismutase
- neonatal
- hexokinase
- gsh
- hemoglobin
-
malic
- plasmodium
- children
- newborn
-
haemolysis
- malate
-
x-linked
- glycogen
- sod
- jaundice
-
sickle
-
lipogenic
- infant
-
shunt
- isocitrate
-
mediterranean
- falciparum
- hyperbilirubinemia
- bilirubin
-
endemic
-
lipogenesis
- transfusion
- vivax
- glucose-6-phosphatase
-
phosphofructokinase
-
cytochemical
-
thiobarbituric
- hexose
- dehydroepiandrosterone
- glucokinase
- glutathione-s-transferase
-
x-chromosome
- phosphoglucomutase
-
phototherapy
- hemoglobinopathy
- methemoglobin
- thalassemia
- synthesis
- beta-thalassemia
- medicine
- drug development
- industry
The enzyme appears in viruses and cellular organisms
Synonyms
6-phosphoglucose dehydrogenase, beta-D-glucose-6-phosphate; NADP oxidoreductase, beta-D-glucose-6-phosphate;NADP oxido-reductase, D-glucose 6-phosphate dehydrogenase, D-glucose 6-phosphate: NADP+ oxidoreductase, D-glucose-6-phosphate: NADP+ oxidoreductase, D-glucose-6-phosphate:NADP oxidoreductase, Entner-Doudoroff enzyme, G-6-PD, G-6-PDH, 
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
6-phosphoglucose dehydrogenase
-
-
-
-
beta-D-glucose-6-phosphate;NADP oxido-reductase
D-glucose 6-phosphate dehydrogenase
-
-
-
-
D-glucose-6-phosphate:NADP oxidoreductase
Entner-Doudoroff enzyme
-
-
-
-
G-6-PD
G-6-PDH
G6PD
G6PD1
G6PD2
G6PD3
G6PD4
G6PD5
G6PD6
G6PDH
G6PDH1
G6PDH2
-
-
-
-
G6PDH3
-
-
-
-
G6PDH4
-
-
-
-
G6PDH5
-
-
-
-
G6PDH6
-
-
-
-
glucose 6-phosphate dehydrogenase
glucose 6-phosphate dehydrogenase (NADP)
-
-
-
-
glucose-6-phosphate 1-dehydrogenase
glucose-6-phosphate dehydrogenase
glucose-6-phosphate dehydrogenase Zwf
NADP-dependent glucose 6-phophate dehydrogenase
-
-
-
-
NADP-glucose-6-phosphate dehydrogenase
VEG11
-
-
-
-
Vegetative protein 11
-
-
-
-
Zwf
zwf-1
Zwf-2
Zwf1p
Zwischenferment
-
-
-
-
G6PD
-
-
-
-
G6PD
-
-
G6PD1
-
-
-
-
G6PD2
-
-
-
-
G6PD3
-
-
-
-
G6PD4
-
-
-
-
G6PD5
-
-
-
-
G6PD6
-
-
-
-
G6PDH1
-
-
-
-
glucose-6-phosphate 1-dehydrogenase
-
-
-
-
glucose-6-phosphate dehydrogenase
-
-
-
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glucose-6-phosphate dehydrogenase
-
NADP-glucose-6-phosphate dehydrogenase
-
-
-
-
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
D-glucose 6-phosphate + NADP+ = 6-phospho-D-glucono-1,5-lactone + NADPH + H+
-
-
-
-
D-glucose 6-phosphate + NADP+ = 6-phospho-D-glucono-1,5-lactone + NADPH + H+
sequential mechanism
-
D-glucose 6-phosphate + NADP+ = 6-phospho-D-glucono-1,5-lactone + NADPH + H+
reaction mechanism is important for regulation
-
D-glucose 6-phosphate + NADP+ = 6-phospho-D-glucono-1,5-lactone + NADPH + H+
rapid equilibrium ordered bi bi kinetic
-
D-glucose 6-phosphate + NADP+ = 6-phospho-D-glucono-1,5-lactone + NADPH + H+
physiological regulating function is discussed
-
D-glucose 6-phosphate + NADP+ = 6-phospho-D-glucono-1,5-lactone + NADPH + H+
rapid-equilibrium random-order mechanism
-
D-glucose 6-phosphate + NADP+ = 6-phospho-D-glucono-1,5-lactone + NADPH + H+
rapid equilibrium random bi bi kinetic mechanism
-
D-glucose 6-phosphate + NADP+ = 6-phospho-D-glucono-1,5-lactone + NADPH + H+
ternary-complex mechanism
-
D-glucose 6-phosphate + NADP+ = 6-phospho-D-glucono-1,5-lactone + NADPH + H+
the adult kidney cortex enzyme shows a ping pong bi bi kinetic mechanism, while the lamb kidney enzyme obeys an ordered bi bi mechanism, thus the kinetic mechanism alters due to aging
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
oxidation
redox reaction
-
-
-
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reduction
-
-
-
-
oxidation
-
-
-
-
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PATHWAY SOURCE
PATHWAYS
BRENDA
KEGG
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SYSTEMATIC NAME
IUBMB Comments
D-glucose-6-phosphate:NADP+ 1-oxidoreductase
The enzyme catalyses a step of the pentose phosphate pathway. The enzyme is specific for NADP+. cf. EC 1.1.1.363, glucose-6-phosphate dehydrogenase [NAD(P)+] and EC 1.1.1.388, glucose-6-phosphate dehydrogenase (NAD+).
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CAS REGISTRY NUMBER
COMMENTARY
9001-40-5
-
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
2-deoxy-D-glucose 6-phosphate + NAD(P)+
2-deoxy-D-glucono-1,5-lactone 6-phosphate + NAD(P)H
-
low activity
-
-
?
2-deoxy-D-glucose-6-phosphate + NADP+
2-deoxy-D-gluconate 6-phosphate + NADPH
-
-
-
-
?
D-glucose + NAD(P)+
D-gluconate + NAD(P)H
-
activity only with dimethylsulfoxide added by 40% (v/v)
-
-
?
D-glucose 6-phosphate + deamino-NADP+
D-glucono-1,5-lactone 6-phosphate + deamino-NADPH
-
-
-
-
?
D-glucose 6-phosphate + NAD+
6-phospho-D-glucono-1,5-lactone + NADP + H+
-
very low activity with NAD+
-
-
?
D-glucose 6-phosphate + NADP+
7-phospho-D-glucono-1,5-lactone + NADPH + H+
-
-
-
-
r
D-glucose 6-phosphate + thio-NAD(P)+
D-glucono-1,5-lactone 6-phosphate + thio-NAD(P)H
-
the thio-cofactors give increased activity compared to the non-thio-cofactors
-
-
?
D-glucose 6-phosphate + thio-NAD+
6-phospho-D-glucono-1,5-lactone + thio-NADH + H+
-
-
-
-
r
2-deoxy-D-glucose 6-phosphate + NADP+
2-deoxy-D-glucono-1,5-lactone 6-phosphate + NADPH
-
-
-
-
?
2-deoxy-D-glucose 6-phosphate + NADP+
2-deoxy-D-glucono-1,5-lactone 6-phosphate + NADPH
-
wild-type and mutant P409R, below 10% activity compared to D-glucose 6-phosphate
-
-
?
D-galactose 6-phosphate + NAD(P)+
D-galactonate 6-phosphate + NADH
-
-
-
-
?
D-glucose 6-phosphate + H2O + NADP+
D-gluconate 6-phosphate + NADPH + H+
-
-
6-phosphoglucono-delta-lactone
?
D-glucose 6-phosphate + H2O + NADP+
D-gluconate 6-phosphate + NADPH + H+
-
-
-
-
r
D-glucose 6-phosphate + H2O + NADP+
D-gluconate 6-phosphate + NADPH + H+
-
-
6-phosphoglucono-delta-lactone
r
D-glucose 6-phosphate + H2O + NADP+
D-gluconate 6-phosphate + NADPH + H+
-
sigmoidal relationship in cofactor binding
-
-
r
D-glucose 6-phosphate + H2O + NADP+
D-gluconate 6-phosphate + NADPH + H+
-
-
6-phosphoglucono-delta-lactone
r
D-glucose 6-phosphate + H2O + NADP+
D-gluconate 6-phosphate + NADPH + H+
-
-
-
r
D-glucose 6-phosphate + H2O + NADP+
D-gluconate 6-phosphate + NADPH + H+
-
-
6-phosphoglucono-delta-lactone
r
D-glucose 6-phosphate + H2O + NADP+
D-gluconate 6-phosphate + NADPH + H+
-
-
6-phosphoglucono-delta-lactone
r
D-glucose 6-phosphate + H2O + NADP+
D-gluconate 6-phosphate + NADPH + H+
-
-
6-phosphoglucono-delta-lactone
r
D-glucose 6-phosphate + H2O + NADP+
D-gluconate 6-phosphate + NADPH + H+
-
-
-
-
?
D-glucose 6-phosphate + H2O + NADP+
D-gluconate 6-phosphate + NADPH + H+
-
-
6-phosphoglucono-delta-lactone
r
D-glucose 6-phosphate + H2O + NADP+
D-gluconate 6-phosphate + NADPH + H+
-
-
6-phosphoglucono-delta-lactone
r
D-glucose 6-phosphate + H2O + NADP+
D-gluconate 6-phosphate + NADPH + H+
-
-
6-phosphoglucono-delta-lactone
r
D-glucose 6-phosphate + H2O + NADP+
D-gluconate 6-phosphate + NADPH + H+
-
-
6-phosphoglucono-delta-lactone
?, ir
D-glucose 6-phosphate + H2O + NADP+
D-gluconate 6-phosphate + NADPH + H+
-
-
6-phosphoglucono-delta-lactone
r
D-glucose 6-phosphate + H2O + NADP+
D-gluconate 6-phosphate + NADPH + H+
-
-
-
?
D-glucose 6-phosphate + H2O + NADP+
D-gluconate 6-phosphate + NADPH + H+
-
-
6-phosphoglucono-delta-lactone
r
D-glucose 6-phosphate + H2O + NADP+
D-gluconate 6-phosphate + NADPH + H+
-
isoform A(-) shows sigmoidal enzyme-substrate interaction, isoform A(+) shows hyperbolic enzyme-substrate interaction
D-glucose-delta-lactone 6-phosphate
r
D-glucose 6-phosphate + H2O + NADP+
D-gluconate 6-phosphate + NADPH + H+
-
hyperbolic cofactor binding
-
?
D-glucose 6-phosphate + H2O + NADP+
D-gluconate 6-phosphate + NADPH + H+
-
-
-
-
?
D-glucose 6-phosphate + H2O + NADP+
D-gluconate 6-phosphate + NADPH + H+
-
-
glucono-delta-lactone-6-phosphate
?
D-glucose 6-phosphate + H2O + NADP+
D-gluconate 6-phosphate + NADPH + H+
-
-
6-phosphoglucono-delta-lactone
r
D-glucose 6-phosphate + H2O + NADP+
D-gluconate 6-phosphate + NADPH + H+
-
-
6-phosphoglucono-delta-lactone
r
D-glucose 6-phosphate + H2O + NADP+
D-gluconate 6-phosphate + NADPH + H+
Penicillium duponti
-
-
6-phosphoglucono-delta-lactone
r
D-glucose 6-phosphate + H2O + NADP+
D-gluconate 6-phosphate + NADPH + H+
-
-
6-phosphoglucono-delta-lactone
?
D-glucose 6-phosphate + H2O + NADP+
D-gluconate 6-phosphate + NADPH + H+
-
-
6-phosphoglucono-delta-lactone
r
D-glucose 6-phosphate + H2O + NADP+
D-gluconate 6-phosphate + NADPH + H+
-
-
6-phosphoglucono-delta-lactone
r
D-glucose 6-phosphate + H2O + NADP+
D-gluconate 6-phosphate + NADPH + H+
-
-
6-phosphoglucono-delta-lactone
r
D-glucose 6-phosphate + H2O + NADP+
D-gluconate 6-phosphate + NADPH + H+
-
-
6-phosphoglucono-delta-lactone
r
D-glucose 6-phosphate + H2O + NADP+
D-gluconate 6-phosphate + NADPH + H+
-
-
6-phosphoglucono-delta-lactone
r
D-glucose 6-phosphate + H2O + NADP+
D-gluconate 6-phosphate + NADPH + H+
-
-
-
-
?
D-glucose 6-phosphate + H2O + NADP+
D-gluconate 6-phosphate + NADPH + H+
-
-
-
ir
D-glucose 6-phosphate + H2O + NADP+
D-gluconate 6-phosphate + NADPH + H+
-
-
-
r
D-glucose 6-phosphate + H2O + NADP+
D-gluconate 6-phosphate + NADPH + H+
-
-
-
-
?
D-glucose 6-phosphate + H2O + NADP+
D-gluconate 6-phosphate + NADPH + H+
-
-
-
-
?
D-glucose 6-phosphate + H2O + NADP+
D-gluconate 6-phosphate + NADPH + H+
-
-
-
?
D-glucose 6-phosphate + H2O + NADP+
D-gluconate 6-phosphate + NADPH + H+
-
-
6-phosphoglucono-delta-lactone
r
D-glucose 6-phosphate + H2O + NADP+
D-gluconate 6-phosphate + NADPH + H+
-
-
6-phosphoglucono-delta-lactone
r
D-glucose 6-phosphate + H2O + NADP+
D-gluconate 6-phosphate + NADPH + H+
-
-
6-phosphoglucono-delta-lactone
r
D-glucose 6-phosphate + NAD(P)+
D-glucono-1,5-lactone 6-phosphate + NAD(P)H
-
key enzyme catalyzing the first step of the pentose phosphate pathway generating NADPH for anabolic metabolsim pathways and protection systems in the liver
-
-
?
D-glucose 6-phosphate + NAD(P)+
D-glucono-1,5-lactone 6-phosphate + NAD(P)H
-
-
-
-
?
D-glucose 6-phosphate + NAD+
6-phospho-D-glucono-1,5-lactone + NADH + H+
-
-
-
-
?
D-glucose 6-phosphate + NAD+
6-phospho-D-glucono-1,5-lactone + NADH + H+
-
NADP+-specific enzyme. In presence of NAD+ 410fold reduction in the performance of the enzyme
-
-
?
D-glucose 6-phosphate + NAD+
6-phospho-D-glucono-1,5-lactone + NADH + H+
-
-
-
-
?
D-glucose 6-phosphate + NAD+
6-phospho-D-glucono-1,5-lactone + NADH + H+
-
-
-
-
?
D-glucose 6-phosphate + NAD+
D-glucono-1,5-lactone 6-phosphate + NADH + H+
-
-
-
-
?
D-glucose 6-phosphate + NAD+
D-glucono-1,5-lactone 6-phosphate + NADH + H+
-
-
-
-
?
D-glucose 6-phosphate + NAD+
D-glucono-1,5-lactone 6-phosphate + NADH + H+
-
-
-
-
?
D-glucose 6-phosphate + NAD+
D-glucono-1,5-lactone 6-phosphate + NADH + H+
-
-
-
-
?
D-glucose 6-phosphate + NAD+
D-glucono-1,5-lactone 6-phosphate + NADH + H+
-
-
-
-
?
D-glucose 6-phosphate + NADP+
6-phospho-D-glucono-1,5-lactone + NADPH + H+
-
-
-
-
r
D-glucose 6-phosphate + NADP+
6-phospho-D-glucono-1,5-lactone + NADPH + H+
-
-
-
-
?
D-glucose 6-phosphate + NADP+
6-phospho-D-glucono-1,5-lactone + NADPH + H+
-
-
-
-
?
D-glucose 6-phosphate + NADP+
6-phospho-D-glucono-1,5-lactone + NADPH + H+
-
-
-
-
?
D-glucose 6-phosphate + NADP+
6-phospho-D-glucono-1,5-lactone + NADPH + H+
-
-
-
-
?
D-glucose 6-phosphate + NADP+
6-phospho-D-glucono-1,5-lactone + NADPH + H+
-
-
-
-
?
D-glucose 6-phosphate + NADP+
6-phospho-D-glucono-1,5-lactone + NADPH + H+
-
first enzyme of the pentose phosphate pathway, one of the main sources of NADPH
-
-
?
D-glucose 6-phosphate + NADP+
6-phospho-D-glucono-1,5-lactone + NADPH + H+
-
oxidative pentose pathway
-
-
?
D-glucose 6-phosphate + NADP+
6-phospho-D-glucono-1,5-lactone + NADPH + H+
-
the enzyme is involved pentose biosynthesis
-
-
?
D-glucose 6-phosphate + NADP+
6-phospho-D-glucono-1,5-lactone + NADPH + H+
-
NADP+-specific enzyme. In presence of NAD+ 410fold reduction in the performance of the enzyme. Modeling of the sensitivity of reduced cofactor production by G6PDH as a function of the redox ratios of NAD/NADH (rRNAD) and NADP/NADPH (rRNADP). NADPH production sharply increases within the range of thermodynamically feasible values of rRNADP, but NADH production remains low within the range feasible for rRNAD
-
-
?
D-glucose 6-phosphate + NADP+
6-phospho-D-glucono-1,5-lactone + NADPH + H+
Escherichia coli B / ATCC 11303
-
-
-
-
?
D-glucose 6-phosphate + NADP+
6-phospho-D-glucono-1,5-lactone + NADPH + H+
Escherichia coli B / ATCC 11303
-
oxidative pentose pathway
-
-
?
D-glucose 6-phosphate + NADP+
6-phospho-D-glucono-1,5-lactone + NADPH + H+
Escherichia coli B / ATCC 11303
-
-
-
-
?
D-glucose 6-phosphate + NADP+
6-phospho-D-glucono-1,5-lactone + NADPH + H+
Escherichia coli B / ATCC 11303
-
the enzyme is involved pentose biosynthesis
-
-
?
D-glucose 6-phosphate + NADP+
6-phospho-D-glucono-1,5-lactone + NADPH + H+
-
-
-
-
?
D-glucose 6-phosphate + NADP+
6-phospho-D-glucono-1,5-lactone + NADPH + H+
-
-
-
-
?
D-glucose 6-phosphate + NADP+
6-phospho-D-glucono-1,5-lactone + NADPH + H+
-
-
-
?
D-glucose 6-phosphate + NADP+
6-phospho-D-glucono-1,5-lactone + NADPH + H+
-
-
-
-
?
D-glucose 6-phosphate + NADP+
6-phospho-D-glucono-1,5-lactone + NADPH + H+
-
-
-
?
D-glucose 6-phosphate + NADP+
6-phospho-D-glucono-1,5-lactone + NADPH + H+
-
-
-
-
?
D-glucose 6-phosphate + NADP+
6-phospho-D-glucono-1,5-lactone + NADPH + H+
-
-
-
?
D-glucose 6-phosphate + NADP+
6-phospho-D-glucono-1,5-lactone + NADPH + H+
-
-
-
-
?
D-glucose 6-phosphate + NADP+
6-phospho-D-glucono-1,5-lactone + NADPH + H+
-
-
-
-
?
D-glucose 6-phosphate + NADP+
6-phospho-D-glucono-1,5-lactone + NADPH + H+
-
-
-
-
?
D-glucose 6-phosphate + NADP+
6-phospho-D-glucono-1,5-lactone + NADPH + H+
-
-
-
-
?
D-glucose 6-phosphate + NADP+
6-phospho-D-glucono-1,5-lactone + NADPH + H+
-
-
-
-
?
D-glucose 6-phosphate + NADP+
6-phospho-D-glucono-1,5-lactone + NADPH + H+
-
-
-
-
?
D-glucose 6-phosphate + NADP+
6-phospho-D-glucono-1,5-lactone + NADPH + H+
-
-
-
-
ir
D-glucose 6-phosphate + NADP+
6-phospho-D-glucono-1,5-lactone + NADPH + H+
-
-
-
-
ir
D-glucose 6-phosphate + NADP+
6-phospho-D-glucono-1,5-lactone + NADPH + H+
-
-
-
-
?
D-glucose 6-phosphate + NADP+
6-phospho-D-glucono-1,5-lactone + NADPH + H+
-
-
-
?
D-glucose 6-phosphate + NADP+
6-phospho-D-glucono-1,5-lactone + NADPH + H+
-
-
-
-
?
D-glucose 6-phosphate + NADP+
6-phospho-D-glucono-1,5-lactone + NADPH + H+
-
-
-
-
?
D-glucose 6-phosphate + NADP+
6-phospho-D-glucono-1,5-lactone + NADPH + H+
-
-
-
?
D-glucose 6-phosphate + NADP+
6-phospho-D-glucono-1,5-lactone + NADPH + H+
-
-
-
?
D-glucose 6-phosphate + NADP+
6-phospho-D-glucono-1,5-lactone + NADPH + H+
-
-
-
?
D-glucose 6-phosphate + NADP+
6-phospho-D-glucono-1,5-lactone + NADPH + H+
-
-
-
?
D-glucose 6-phosphate + NADP+
D-glucono-1,5-lactone 6-phosphate + NADPH
-
-
-
-
?
D-glucose 6-phosphate + NADP+
D-glucono-1,5-lactone 6-phosphate + NADPH
-
-
-
?
D-glucose 6-phosphate + NADP+
D-glucono-1,5-lactone 6-phosphate + NADPH
-
-
-
?
D-glucose 6-phosphate + NADP+
D-glucono-1,5-lactone 6-phosphate + NADPH
-
-
-
?
D-glucose 6-phosphate + NADP+
D-glucono-1,5-lactone 6-phosphate + NADPH
-
-
-
?
D-glucose 6-phosphate + NADP+
D-glucono-1,5-lactone 6-phosphate + NADPH
-
inhibition by divalent metal ions, at low NADPH /NADP+ ratio, is another means of controlling the pentose phosphate pathway
-
-
?
D-glucose 6-phosphate + NADP+
D-glucono-1,5-lactone 6-phosphate + NADPH
-
the substrate specificity is extremely strict
-
-
?
D-glucose 6-phosphate + NADP+
D-glucono-1,5-lactone 6-phosphate + NADPH
-
-
-
-
?
D-glucose 6-phosphate + NADP+
D-glucono-1,5-lactone 6-phosphate + NADPH
-
-
-
-
?
D-glucose 6-phosphate + NADP+
D-glucono-1,5-lactone 6-phosphate + NADPH
-
-
-
-
?
D-glucose 6-phosphate + NADP+
D-glucono-1,5-lactone 6-phosphate + NADPH
-
catalyzes the first step of the pentose phosphate pathway, enzyme deficiency often causes hemolytic anaemia
-
-
?
D-glucose 6-phosphate + NADP+
D-glucono-1,5-lactone 6-phosphate + NADPH
-
key enzyme catalyzing the first step in the pentose phosphate pathway
-
-
?
D-glucose 6-phosphate + NADP+
D-glucono-1,5-lactone 6-phosphate + NADPH
-
wild-type and mutant P409R
-
-
?
D-glucose 6-phosphate + NADP+
D-glucono-1,5-lactone 6-phosphate + NADPH
-
-
-
-
?
D-glucose 6-phosphate + NADP+
D-glucono-1,5-lactone 6-phosphate + NADPH
-
involved in oxidative pentose phosphate pathway in roots, related to glutamate synthesis in the plastids
-
-
?
D-glucose 6-phosphate + NADP+
D-glucono-1,5-lactone 6-phosphate + NADPH
-
-
-
-
?
D-glucose 6-phosphate + NADP+
D-glucono-1,5-lactone 6-phosphate + NADPH
-
-
-
-
?
D-glucose 6-phosphate + NADP+
D-glucono-1,5-lactone 6-phosphate + NADPH
-
key enzyme of the pentose phosphate pathway
-
-
?
D-glucose 6-phosphate + NADP+
D-glucono-1,5-lactone 6-phosphate + NADPH
-
-
-
-
?
D-glucose 6-phosphate + NADP+
D-glucono-1,5-lactone 6-phosphate + NADPH
-
-
-
-
?
D-glucose 6-phosphate + NADP+
D-glucono-1,5-lactone 6-phosphate + NADPH
-
-
-
-
?
D-glucose 6-phosphate + NADP+
D-glucono-1,5-lactone 6-phosphate + NADPH
-
G6PDH is the rate-limiting step in the formation of NADPH via the triphosphate, and is responsible for the major supply of NADPH in the cell
-
-
?
D-glucose 6-phosphate + NADP+
D-glucono-1,5-lactone 6-phosphate + NADPH
-
-
-
-
?
D-glucose 6-phosphate + NADP+
D-glucono-1,5-lactone 6-phosphate + NADPH
-
-
-
-
?
D-glucose 6-phosphate + NADP+
D-glucono-1,5-lactone 6-phosphate + NADPH
-
first step in the pentose phosphate pathway
-
-
?
D-glucose 6-phosphate + NADP+
D-glucono-1,5-lactone 6-phosphate + NADPH
-
key enzyme catalyzing the first step of the hexose monophosphate shunt pathway
-
-
?
D-glucose 6-phosphate + NADP+
D-glucono-1,5-lactone 6-phosphate + NADPH
-
-
-
-
?
D-glucose 6-phosphate + NADP+
D-glucono-1,5-lactone 6-phosphate + NADPH
-
-
-
-
?
D-glucose 6-phosphate + NADP+
D-glucono-1,5-lactone 6-phosphate + NADPH
-
-
-
-
?
D-glucose 6-phosphate + NADP+
D-glucono-1,5-lactone 6-phosphate + NADPH
-
-
-
-
?
D-glucose 6-phosphate + NADP+
D-glucono-1,5-lactone 6-phosphate + NADPH
-
-
-
-
?
D-glucose 6-phosphate + NADP+
D-glucono-1,5-lactone 6-phosphate + NADPH
-
-
-
-
?
D-glucose 6-phosphate + NADP+
D-glucono-1,5-lactone 6-phosphate + NADPH
-
enzyme catalyzes the first step of the pentose phosphate pathway
-
-
?
D-glucose 6-phosphate + NADP+
D-glucono-1,5-lactone 6-phosphate + NADPH
-
key regulatory enzyme of the pentose phosphate pathway, regulation mechanism study
-
-
?
D-glucose 6-phosphate + NADP+
D-glucono-1,5-lactone 6-phosphate + NADPH
-
-
-
-
?
D-glucose 6-phosphate + NADP+
D-glucono-1,5-lactone 6-phosphate + NADPH
-
differential regulation of isozymes, the cytosolic enzyme is regulated by sugar availability at transcriptional level, while the plastidic isozymes are regulated by redox mechanism
-
-
?
D-glucose 6-phosphate + NADP+
D-glucono-1,5-lactone 6-phosphate + NADPH
-
-
-
-
?
D-glucose 6-phosphate + NADP+
D-glucono-1,5-lactone 6-phosphate + NADPH
-
-
-
-
?
D-glucose 6-phosphate + NADP+
D-glucono-1,5-lactone 6-phosphate + NADPH
-
-
-
-
?
D-glucose 6-phosphate + NADP+
D-glucono-1,5-lactone 6-phosphate + NADPH + H+
-
-
-
-
?
D-glucose 6-phosphate + NADP+
D-glucono-1,5-lactone 6-phosphate + NADPH + H+
-
-
-
?
D-glucose 6-phosphate + NADP+
D-glucono-1,5-lactone 6-phosphate + NADPH + H+
-
-
-
-
?
D-glucose 6-phosphate + NADP+
D-glucono-1,5-lactone 6-phosphate + NADPH + H+
-
-
-
-
?
D-glucose 6-phosphate + NADP+
D-glucono-1,5-lactone 6-phosphate + NADPH + H+
-
-
-
?
D-glucose 6-phosphate + NADP+
D-glucono-1,5-lactone 6-phosphate + NADPH + H+
-
-
-
-
?
D-glucose 6-phosphate + NADP+
D-glucono-1,5-lactone 6-phosphate + NADPH + H+
-
-
-
-
?
D-glucose 6-phosphate + NADP+
D-glucono-1,5-lactone 6-phosphate + NADPH + H+
-
-
-
-
?
D-glucose 6-phosphate + NADP+
D-glucono-1,5-lactone 6-phosphate + NADPH + H+
-
oxidative enzyme of pentose phosphate pathway
-
-
?
D-glucose 6-phosphate + NADP+
D-glucono-1,5-lactone 6-phosphate + NADPH + H+
-
-
-
-
?
D-glucose 6-phosphate + NADP+
D-glucono-1,5-lactone 6-phosphate + NADPH + H+
-
-
-
-
?
D-glucose 6-phosphate + NADP+
D-glucono-1,5-lactone 6-phosphate + NADPH + H+
-
-
-
-
?
D-glucose 6-phosphate + NADP+
D-glucono-1,5-lactone 6-phosphate + NADPH + H+
-
-
-
-
?
D-glucose 6-phosphate + NADP+
D-glucono-1,5-lactone 6-phosphate + NADPH + H+
-
-
-
-
?
D-glucose 6-phosphate + NADP+
D-glucono-1,5-lactone 6-phosphate + NADPH + H+
-
-
-
-
?
D-glucose 6-phosphate + NADP+
D-glucono-1,5-lactone 6-phosphate + NADPH + H+
-
random bi bi sequential mechanism in rapid equilibrium
-
-
?
D-glucose 6-phosphate + NADP+
D-glucono-1,5-lactone 6-phosphate + NADPH + H+
-
-
-
?
D-glucose 6-phosphate + NADP+ + H2O
D-gluconate 6-phosphate + NADPH + H+
-
-
-
-
?
D-glucose 6-phosphate + NADP+ + H2O
D-gluconate 6-phosphate + NADPH + H+
-
the enzyme is responsible for the production of NADPH, which is required for biosynthetic reactions and protection of cells from free radicals
-
-
?
D-glucose 6-phosphate + NADP+ + H2O
D-gluconate 6-phosphate + NADPH + H+
-
-
-
-
?
D-glucose 6-phosphate + NADP+ + H2O
D-gluconate 6-phosphate + NADPH + H+
-
-
-
-
?
D-glucose 6-phosphate + NADP+ + H2O
D-gluconate 6-phosphate + NADPH + H+
-
-
-
-
?
additional information
?
-
-
G6PDH plays a role in supplying NADPH for oil accumulation in developing seeds in which photosynthesis may be light limited
-
-
?
additional information
?
-
-
no activity with glucose and glucosamine 6-phosphate
-
-
?
additional information
?
-
-
no activity with glucose, glucose 1-phosphate, 2-deoxyglucose 6-phosphate, fructose, fructose 6-phosphate, fructose 1,6-diphosphate, mannose, galactose 6-phosphate, NAD+
-
-
?
additional information
?
-
-
no activity with glucose, glucose 1-phosphate, 2-deoxyglucose 6-phosphate, fructose, fructose 6-phosphate, fructose 1,6-diphosphate, mannose, galactose 6-phosphate, NAD+
-
-
?
additional information
?
-
-
the following substances, 1 mM, are not substrates when substituted for D-glucose 6-phosphate: D-glucose 6-phosphate, D-fructose 1-phosphate, D-gluconate 6-phosphate, D-galactose 6-phosphate, D-ribose 5-phosphate, and D-glucose
-
-
?
additional information
?
-
-
the following substances, 1 mM, are not substrates when substituted for D-glucose 6-phosphate: D-glucose 6-phosphate, D-fructose 1-phosphate, D-gluconate 6-phosphate, D-galactose 6-phosphate, D-ribose 5-phosphate, and D-glucose
-
-
?
additional information
?
-
-
the following substances, 1 mM, are not substrates when substituted for D-glucose 6-phosphate: D-glucose 6-phosphate, D-fructose 1-phosphate, D-gluconate 6-phosphate, D-galactose 6-phosphate, D-ribose 5-phosphate, and D-glucose
-
-
?
additional information
?
-
-
both isoenzymes show no activity with glucose and NAD+, isoenzyme I shows no activity with 6-phosphonogluconate
-
-
?
additional information
?
-
-
no activity with other sugars, such as fructose-6-phosphate, glucose-1-phosphate, ribulose 5-phosphate, and ribose 5-phosphate
-
-
?
additional information
?
-
-
G6PD overexpressed in adipocytes in obese (including db/db, ob/ob and diet-induced obesity) mice, in adipocytes and stromal-vascular cells in diabetic db/db mice, and in adipocytes cultured under high-glucose conditions in vitro induces the expression of NADPH oxidase and leads to an increase in oxidative stress
-
-
?
additional information
?
-
-
transgenic (previously susceptible) lines overexpressing G6PDH-encoding gene, displaying high NADPH tolerance engineered for cytosolic expression, show early oxidative bursts, callose deposition, and changes in metabolic parameter
-
-
?
additional information
?
-
-
G6PDH plays a critical role in maintaining cellular reduced glutathione levels under long-term salt stress in plants. NADPH, produced by G6PDH, not only acts as the reducing potential for the output of reduced glutathione, but is involved in the activity of plasma membrane NADPH oxidase under salt stress, which results in hydrogen peroxide accumulation
-
-
?
additional information
?
-
-
the enzyme is a bifunctional enzyme glucose-6-phosphate dehydrogenase-6-phosphogluconolactonase, the enzyme activity is analyzed for the reaction of glucose-6-phosphate dehydrogenase, EC 1.1.1.49, in a coupled assay with diaphorase activity, which uses NADPH to reduce resazurin to form the highly fluorescent molecule resorufin
-
-
?
additional information
?
-
-
physiological regulation of NADP+-NADPH-ratio in the brain is dicussed
-
-
?
additional information
?
-
-
no activity with D-glucose, D-ribose, D-fructose-6-phosphate, D-ribose-5-phosphate
-
-
?
additional information
?
-
-
no activity with D-glucose, D-ribose, D-fructose-6-phosphate, D-ribose-5-phosphate
-
-
?
additional information
?
-
-
both isoenzymes show no activity with glucose and NAD+, isoenzyme I shows no activity with 6-phosphonogluconate
-
-
?
additional information
?
-
-
glucose-6-phosphate dehydrogenase protects the parasite against reactive oxygen species
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
D-glucose 6-phosphate + NAD(P)+
D-glucono-1,5-lactone 6-phosphate + NAD(P)H
-
key enzyme catalyzing the first step of the pentose phosphate pathway generating NADPH for anabolic metabolsim pathways and protection systems in the liver
-
-
?
D-glucose 6-phosphate + H2O + NADP+
D-gluconate 6-phosphate + NADPH + H+
-
-
6-phosphoglucono-delta-lactone
?
D-glucose 6-phosphate + H2O + NADP+
D-gluconate 6-phosphate + NADPH + H+
-
-
6-phosphoglucono-delta-lactone
r
D-glucose 6-phosphate + H2O + NADP+
D-gluconate 6-phosphate + NADPH + H+
-
sigmoidal relationship in cofactor binding
-
-
r
D-glucose 6-phosphate + H2O + NADP+
D-gluconate 6-phosphate + NADPH + H+
-
-
6-phosphoglucono-delta-lactone
r
D-glucose 6-phosphate + H2O + NADP+
D-gluconate 6-phosphate + NADPH + H+
-
-
-
r
D-glucose 6-phosphate + H2O + NADP+
D-gluconate 6-phosphate + NADPH + H+
-
-
6-phosphoglucono-delta-lactone
r
D-glucose 6-phosphate + H2O + NADP+
D-gluconate 6-phosphate + NADPH + H+
-
-
6-phosphoglucono-delta-lactone
r
D-glucose 6-phosphate + H2O + NADP+
D-gluconate 6-phosphate + NADPH + H+
-
-
6-phosphoglucono-delta-lactone
r
D-glucose 6-phosphate + H2O + NADP+
D-gluconate 6-phosphate + NADPH + H+
-
-
-
-
?
D-glucose 6-phosphate + H2O + NADP+
D-gluconate 6-phosphate + NADPH + H+
-
-
6-phosphoglucono-delta-lactone
r
D-glucose 6-phosphate + H2O + NADP+
D-gluconate 6-phosphate + NADPH + H+
-
-
6-phosphoglucono-delta-lactone
r
D-glucose 6-phosphate + H2O + NADP+
D-gluconate 6-phosphate + NADPH + H+
-
-
6-phosphoglucono-delta-lactone
r
D-glucose 6-phosphate + H2O + NADP+
D-gluconate 6-phosphate + NADPH + H+
-
-
6-phosphoglucono-delta-lactone
?
D-glucose 6-phosphate + H2O + NADP+
D-gluconate 6-phosphate + NADPH + H+
-
-
6-phosphoglucono-delta-lactone
r
D-glucose 6-phosphate + H2O + NADP+
D-gluconate 6-phosphate + NADPH + H+
-
-
-
-
?
D-glucose 6-phosphate + H2O + NADP+
D-gluconate 6-phosphate + NADPH + H+
-
-
6-phosphoglucono-delta-lactone
r
D-glucose 6-phosphate + H2O + NADP+
D-gluconate 6-phosphate + NADPH + H+
-
isoform A(-) shows sigmoidal enzyme-substrate interaction, isoform A(+) shows hyperbolic enzyme-substrate interaction
D-glucose-delta-lactone 6-phosphate
r
D-glucose 6-phosphate + H2O + NADP+
D-gluconate 6-phosphate + NADPH + H+
-
hyperbolic cofactor binding
-
-
?
D-glucose 6-phosphate + H2O + NADP+
D-gluconate 6-phosphate + NADPH + H+
-
-
-
-
?
D-glucose 6-phosphate + H2O + NADP+
D-gluconate 6-phosphate + NADPH + H+
-
-
6-phosphoglucono-delta-lactone
r
D-glucose 6-phosphate + H2O + NADP+
D-gluconate 6-phosphate + NADPH + H+
-
-
6-phosphoglucono-delta-lactone
r
D-glucose 6-phosphate + H2O + NADP+
D-gluconate 6-phosphate + NADPH + H+
Penicillium duponti
-
-
6-phosphoglucono-delta-lactone
r
D-glucose 6-phosphate + H2O + NADP+
D-gluconate 6-phosphate + NADPH + H+
-
-
6-phosphoglucono-delta-lactone
?
D-glucose 6-phosphate + H2O + NADP+
D-gluconate 6-phosphate + NADPH + H+
-
-
6-phosphoglucono-delta-lactone
r
D-glucose 6-phosphate + H2O + NADP+
D-gluconate 6-phosphate + NADPH + H+
-
-
6-phosphoglucono-delta-lactone
r
D-glucose 6-phosphate + H2O + NADP+
D-gluconate 6-phosphate + NADPH + H+
-
-
6-phosphoglucono-delta-lactone
r
D-glucose 6-phosphate + H2O + NADP+
D-gluconate 6-phosphate + NADPH + H+
-
-
6-phosphoglucono-delta-lactone
r
D-glucose 6-phosphate + H2O + NADP+
D-gluconate 6-phosphate + NADPH + H+
-
-
6-phosphoglucono-delta-lactone
r
D-glucose 6-phosphate + H2O + NADP+
D-gluconate 6-phosphate + NADPH + H+
-
-
-
-
?
D-glucose 6-phosphate + H2O + NADP+
D-gluconate 6-phosphate + NADPH + H+
-
-
-
r
D-glucose 6-phosphate + H2O + NADP+
D-gluconate 6-phosphate + NADPH + H+
-
-
-
-
?
D-glucose 6-phosphate + H2O + NADP+
D-gluconate 6-phosphate + NADPH + H+
-
-
-
-
?
D-glucose 6-phosphate + H2O + NADP+
D-gluconate 6-phosphate + NADPH + H+
-
-
-
-
?
D-glucose 6-phosphate + H2O + NADP+
D-gluconate 6-phosphate + NADPH + H+
-
-
6-phosphoglucono-delta-lactone
r
D-glucose 6-phosphate + H2O + NADP+
D-gluconate 6-phosphate + NADPH + H+
-
-
6-phosphoglucono-delta-lactone
r
D-glucose 6-phosphate + H2O + NADP+
D-gluconate 6-phosphate + NADPH + H+
-
-
6-phosphoglucono-delta-lactone
r
D-glucose 6-phosphate + NADP+
6-phospho-D-glucono-1,5-lactone + NADPH + H+
-
-
-
-
?
D-glucose 6-phosphate + NADP+
6-phospho-D-glucono-1,5-lactone + NADPH + H+
-
-
-
-
?
D-glucose 6-phosphate + NADP+
6-phospho-D-glucono-1,5-lactone + NADPH + H+
-
-
-
-
?
D-glucose 6-phosphate + NADP+
6-phospho-D-glucono-1,5-lactone + NADPH + H+
-
-
-
-
?
D-glucose 6-phosphate + NADP+
6-phospho-D-glucono-1,5-lactone + NADPH + H+
-
-
-
-
?
D-glucose 6-phosphate + NADP+
6-phospho-D-glucono-1,5-lactone + NADPH + H+
-
first enzyme of the pentose phosphate pathway, one of the main sources of NADPH
-
-
?
D-glucose 6-phosphate + NADP+
6-phospho-D-glucono-1,5-lactone + NADPH + H+
-
oxidative pentose pathway
-
-
?
D-glucose 6-phosphate + NADP+
6-phospho-D-glucono-1,5-lactone + NADPH + H+
-
the enzyme is involved pentose biosynthesis
-
-
?
D-glucose 6-phosphate + NADP+
6-phospho-D-glucono-1,5-lactone + NADPH + H+
Escherichia coli B / ATCC 11303
-
oxidative pentose pathway
-
-
?
D-glucose 6-phosphate + NADP+
6-phospho-D-glucono-1,5-lactone + NADPH + H+
Escherichia coli B / ATCC 11303
-
the enzyme is involved pentose biosynthesis
-
-
?
D-glucose 6-phosphate + NADP+
6-phospho-D-glucono-1,5-lactone + NADPH + H+
-
-
-
-
?
D-glucose 6-phosphate + NADP+
6-phospho-D-glucono-1,5-lactone + NADPH + H+
-
-
-
-
?
D-glucose 6-phosphate + NADP+
6-phospho-D-glucono-1,5-lactone + NADPH + H+
-
-
-
?
D-glucose 6-phosphate + NADP+
6-phospho-D-glucono-1,5-lactone + NADPH + H+
-
-
-
-
?
D-glucose 6-phosphate + NADP+
6-phospho-D-glucono-1,5-lactone + NADPH + H+
-
-
-
?
D-glucose 6-phosphate + NADP+
6-phospho-D-glucono-1,5-lactone + NADPH + H+
-
-
-
-
?
D-glucose 6-phosphate + NADP+
6-phospho-D-glucono-1,5-lactone + NADPH + H+
-
-
-
?
D-glucose 6-phosphate + NADP+
6-phospho-D-glucono-1,5-lactone + NADPH + H+
-
-
-
-
?
D-glucose 6-phosphate + NADP+
6-phospho-D-glucono-1,5-lactone + NADPH + H+
-
-
-
-
?
D-glucose 6-phosphate + NADP+
6-phospho-D-glucono-1,5-lactone + NADPH + H+
-
-
-
-
?
D-glucose 6-phosphate + NADP+
6-phospho-D-glucono-1,5-lactone + NADPH + H+
-
-
-
-
?
D-glucose 6-phosphate + NADP+
6-phospho-D-glucono-1,5-lactone + NADPH + H+
-
-
-
-
?
D-glucose 6-phosphate + NADP+
6-phospho-D-glucono-1,5-lactone + NADPH + H+
-
-
-
-
?
D-glucose 6-phosphate + NADP+
6-phospho-D-glucono-1,5-lactone + NADPH + H+
-
-
-
-
ir
D-glucose 6-phosphate + NADP+
6-phospho-D-glucono-1,5-lactone + NADPH + H+
-
-
-
-
ir
D-glucose 6-phosphate + NADP+
6-phospho-D-glucono-1,5-lactone + NADPH + H+
-
-
-
-
?
D-glucose 6-phosphate + NADP+
6-phospho-D-glucono-1,5-lactone + NADPH + H+
-
-
-
?
D-glucose 6-phosphate + NADP+
6-phospho-D-glucono-1,5-lactone + NADPH + H+
-
-
-
-
?
D-glucose 6-phosphate + NADP+
6-phospho-D-glucono-1,5-lactone + NADPH + H+
-
-
-
-
?
D-glucose 6-phosphate + NADP+
6-phospho-D-glucono-1,5-lactone + NADPH + H+
-
-
-
?
D-glucose 6-phosphate + NADP+
6-phospho-D-glucono-1,5-lactone + NADPH + H+
-
-
-
?
D-glucose 6-phosphate + NADP+
6-phospho-D-glucono-1,5-lactone + NADPH + H+
-
-
-
?
D-glucose 6-phosphate + NADP+
6-phospho-D-glucono-1,5-lactone + NADPH + H+
-
-
-
?
D-glucose 6-phosphate + NADP+
D-glucono-1,5-lactone 6-phosphate + NADPH
-
-
-
-
?
D-glucose 6-phosphate + NADP+
D-glucono-1,5-lactone 6-phosphate + NADPH
-
inhibition by divalent metal ions, at low NADPH /NADP+ ratio, is another means of controlling the pentose phosphate pathway
-
-
?
D-glucose 6-phosphate + NADP+
D-glucono-1,5-lactone 6-phosphate + NADPH
-
-
-
-
?
D-glucose 6-phosphate + NADP+
D-glucono-1,5-lactone 6-phosphate + NADPH
-
catalyzes the first step of the pentose phosphate pathway, enzyme deficiency often causes hemolytic anaemia
-
-
?
D-glucose 6-phosphate + NADP+
D-glucono-1,5-lactone 6-phosphate + NADPH
-
key enzyme catalyzing the first step in the pentose phosphate pathway
-
-
?
D-glucose 6-phosphate + NADP+
D-glucono-1,5-lactone 6-phosphate + NADPH
-
involved in oxidative pentose phosphate pathway in roots, related to glutamate synthesis in the plastids
-
-
?
D-glucose 6-phosphate + NADP+
D-glucono-1,5-lactone 6-phosphate + NADPH
-
key enzyme of the pentose phosphate pathway
-
-
?
D-glucose 6-phosphate + NADP+
D-glucono-1,5-lactone 6-phosphate + NADPH
-
-
-
-
?
D-glucose 6-phosphate + NADP+
D-glucono-1,5-lactone 6-phosphate + NADPH
-
-
-
-
?
D-glucose 6-phosphate + NADP+
D-glucono-1,5-lactone 6-phosphate + NADPH
-
G6PDH is the rate-limiting step in the formation of NADPH via the triphosphate, and is responsible for the major supply of NADPH in the cell
-
-
?
D-glucose 6-phosphate + NADP+
D-glucono-1,5-lactone 6-phosphate + NADPH
-
first step in the pentose phosphate pathway
-
-
?
D-glucose 6-phosphate + NADP+
D-glucono-1,5-lactone 6-phosphate + NADPH
-
key enzyme catalyzing the first step of the hexose monophosphate shunt pathway
-
-
?
D-glucose 6-phosphate + NADP+
D-glucono-1,5-lactone 6-phosphate + NADPH
-
-
-
-
?
D-glucose 6-phosphate + NADP+
D-glucono-1,5-lactone 6-phosphate + NADPH
-
-
-
-
?
D-glucose 6-phosphate + NADP+
D-glucono-1,5-lactone 6-phosphate + NADPH
-
-
-
-
?
D-glucose 6-phosphate + NADP+
D-glucono-1,5-lactone 6-phosphate + NADPH
-
enzyme catalyzes the first step of the pentose phosphate pathway
-
-
?
D-glucose 6-phosphate + NADP+
D-glucono-1,5-lactone 6-phosphate + NADPH
-
key regulatory enzyme of the pentose phosphate pathway, regulation mechanism study
-
-
?
D-glucose 6-phosphate + NADP+
D-glucono-1,5-lactone 6-phosphate + NADPH
-
differential regulation of isozymes, the cytosolic enzyme is regulated by sugar availability at transcriptional level, while the plastidic isozymes are regulated by redox mechanism
-
-
?
D-glucose 6-phosphate + NADP+
D-glucono-1,5-lactone 6-phosphate + NADPH
-
-
-
-
?
D-glucose 6-phosphate + NADP+
D-glucono-1,5-lactone 6-phosphate + NADPH + H+
-
-
-
-
?
D-glucose 6-phosphate + NADP+
D-glucono-1,5-lactone 6-phosphate + NADPH + H+
-
-
-
?
D-glucose 6-phosphate + NADP+
D-glucono-1,5-lactone 6-phosphate + NADPH + H+
-
-
-
-
?
D-glucose 6-phosphate + NADP+
D-glucono-1,5-lactone 6-phosphate + NADPH + H+
-
-
-
-
?
D-glucose 6-phosphate + NADP+
D-glucono-1,5-lactone 6-phosphate + NADPH + H+
-
-
-
-
?
D-glucose 6-phosphate + NADP+
D-glucono-1,5-lactone 6-phosphate + NADPH + H+
-
oxidative enzyme of pentose phosphate pathway
-
-
?
D-glucose 6-phosphate + NADP+
D-glucono-1,5-lactone 6-phosphate + NADPH + H+
-
-
-
-
?
D-glucose 6-phosphate + NADP+
D-glucono-1,5-lactone 6-phosphate + NADPH + H+
-
-
-
?
D-glucose 6-phosphate + NADP+ + H2O
D-gluconate 6-phosphate + NADPH + H+
-
the enzyme is responsible for the production of NADPH, which is required for biosynthetic reactions and protection of cells from free radicals
-
-
?
D-glucose 6-phosphate + NADP+ + H2O
D-gluconate 6-phosphate + NADPH + H+
-
-
-
-
?
D-glucose 6-phosphate + NADP+ + H2O
D-gluconate 6-phosphate + NADPH + H+
-
-
-
-
?
additional information
?
-
-
the enzyme is a bifunctional enzyme glucose-6-phosphate dehydrogenase-6-phosphogluconolactonase, the enzyme activity is analyzed for the reaction of glucose-6-phosphate dehydrogenase, EC 1.1.1.49, in a coupled assay with diaphorase activity, which uses NADPH to reduce resazurin to form the highly fluorescent molecule resorufin
-
-
?
additional information
?
-
-
physiological regulation of NADP+-NADPH-ratio in the brain is dicussed
-
-
?
additional information
?
-
-
glucose-6-phosphate dehydrogenase protects the parasite against reactive oxygen species
-
-
?
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
NAD+
-
NAD+ can replace NADP+, with 10 mM NAD+ giving 25% of the rate in the usual assay with NADP+
NAD+
-
NADP+-specific enzyme. In presence of NAD+ 410fold reduction in the performance of the enzyme
NAD+
-
the enzyme prefers NADP+ as a coenzyme, although NAD+ is also accepted
NADP+
-
highly preferred cofactor, 1000fold higher activity than with NAD+
NADP+
-
preferred cofactor, 20.4fold higher activity at 40°C and 5.7fold at 70°C than with NAD+
NADP+
the enzyme contains 1 mol NADP+ per subunit. Apoenzyme, devoid of bound NADP+, is still fully active
NADP+
-
G6PD activity is regulated by the intracellular NADP+ levels and the NADPH/NADP+ ratio
NADP+
-
NADP+ has major positive influence on refolding. NADP+ is essential for the folding intermediate to adopt native structure. Without added NADP+, the recovery yield is only about 10%. As the concentration of NADP+ increases, more enzyme activity can be regained in a concentration dependent manner
NADP+
-
almost specific for NADP+ as coenzyme. In presence of 2 and 3 mM NAD+ the velocity is about 2% compared to NADP+
NADP+
-
NADP+-specific enzyme. In presence of NAD+ 410fold reduction in the performance of the enzyme. Modeling of the sensitivity of reduced cofactor production by G6PDH as a function of the redox ratios of NAD/NADH (rRNAD) and NADP/NADPH (rRNADP). NADPH production sharply increases within the range of thermodynamically feasible values of rRNADP, but NADH production remains low within the range feasible for rRNAD
NADP+
the enzyme has two NADP+ binding sites, one is the structural NADP+ and the second is the catalytic NADP+. The structural NADP+ domain adopt a classic beta-alpha-beta dinucleotide fold with the fingerprint sequence GASGDLA at the tight turn following the first beta-strand
NADP+
-
the enzyme prefers NADP+ as a coenzyme, although NAD+ is also accepted
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Ca2+
Cd2+
Co2+
Mg2+
Mn2+
Ni2+
-
about 110% activity at 5 mM (isoform G6PD2), about 115% activity at 2 mM (isoform G6PD1)
nitrate
-
activates plastidic isozymes by 10fold by direct action on the enzyme
nitrite
-
activates plastidic isozymes by 10fold by direct action on the enzyme
sulfate
-
activates plastidic isozymes by 10fold by direct action on the enzyme
additional information
Cd2+
-
enzyme is stimulated in presence of 3 mg and 5 mg of Cd on the first day of experiment in gill, liver and kidney tissues. The stimulation effect of the 5 mg/l dose of Cd on G6PD and 6PGD enzyme activities is significantly diminished after seven days. The G6PDenzyme activity levels are stimulated by approximately 60% in gills, 68% in liver, and 67% in kidneys
Mg2+
-
about 130% activity at 5 mM (isoform G6PD2), about 150% activity at 5 mM (isoform G6PD1)
Mg2+
-
activates, no absolute requirement. In the presence of saturating concentrations of spermidine or other polycations, Mg2+ ions have no further stimulatory effect
Mg2+
-
is markedly stimulated by (but not absolutely dependent upon) Mg2+
Mn2+
-
is markedly stimulated by (but not absolutely dependent upon) Mn2+
additional information
-
0.87 and 3 ppm Fe3+ concentrations have no significant effect on enzyme activity
additional information
-
no effect on cytosolic isozyme by H2O2, FeCl3, NH4Cl, NaNO2, NaNO3, sulfate or endothall, okadaic acid and NaF
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INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
(2R,3R)-2-(3,4-dihydroxyphenyl)-5,7-dihydroxy-3,4-dihydro-2H-chromen-3-yl 3,4,5-trihydroxybenzoate
-
-
(2R,3R)-5,7-dihydroxy-2-(3,4,5-trihydroxyphenyl)-3,4-dihydro-2H-chromen-3-yl 3,4,5-trihydroxybenzoate
-
-
(2S,3R)-2-(3,4-dihydroxyphenyl)-5,7-dihydroxy-3,4-dihydro-2H-chromen-3-yl 3,4,5-trihydroxybenzoate
-
-
(2S,3R)-5,7-dihydroxy-2-(3,4,5-trihydroxyphenyl)-3,4-dihydro-2H-chromen-3-yl 3,4,5-trihydroxybenzoate
-
-
(4Z)-4-(3-bromobenzylidene)-1-(4-methylphenyl)pyrazolidine-3,5-dione
-
-
(5Z)-1-(4-bromophenyl)-5-[[5-(2-nitrophenyl)furan-2-yl]methylidene]pyrimidine-2,4,6(1H,3H,5H)-trione
-
-
(5Z)-1-(4-ethoxyphenyl)-5-[[5-(2-nitrophenyl)furan-2-yl]methylidene]pyrimidine-2,4,6(1H,3H,5H)-trione
-
-
(5Z)-5-[[5-(2-methyl-4-nitrophenyl)furan-2-yl]methylidene]-1-(3-methylphenyl)pyrimidine-2,4,6(1H,3H,5H)-trione
-
reversible, non-competitive inhibition versus NADP+, mixed-type versus D-glucose 6-phosphate
1-(2-nitro-1-(thiophen-2-yl)ethyl)-1H-indole
competitive inhibitor
-
2-[(5Z)-5-(4-hydroxy-3-methoxybenzylidene)-4-oxo-2-thioxo-1,3-thiazolidin-3-yl]-3-phenylpropanoate
-
-
2-[(E)-(4-nitrocyclohexa-2,4-dien-1-yl)(2-phenylhydrazinylidene)methyl]benzene-1,4-diol
-
non-competitive inhibition versus NADP+, non-competitive or mixed-type versus D-glucose 6-phosphate
2-[4-(4-chlorophenyl)-1,3-thiazol-2-yl]-5-methyl-4-(3,4,5-trimethoxybenzyl)-2,4-dihydro-3H-pyrazol-3-one
-
irreversible, non-competitive inhibition versus NADP+, mixed-type versus D-glucose 6-phosphate
3-(3,4-dichlorophenyl)-1,1'-dimethyl urea
-
cytosolic isozyme, inhibition by uncoupling of photosynthetic electron transport
3-(4-hydroxyphenyl)-2-(pyridin-3-yl)quinazolin-4(3H)-one
-
irreversible, non-competitive inhibition versus NADP+, mixed-type versus D-glucose 6-phosphate
3-(5-bromopyridin-2-yl)-2-(pyridin-3-yl)quinazolin-4(3H)-one
-
irreversible, non-competitive inhibition versus NADP+, mixed-type versus D-glucose 6-phosphate
3-(5-[(E)-[2-(5-nitropyridin-2-yl)hydrazinylidene]methyl]furan-2-yl)benzoic acid
-
-
4-methyl-3-(propan-2-yloxy)-6H-benzo[c]chromen-6-one
-
non-competitive inhibition versus NADP+, mixed-type versus D-glucose 6-phosphate
5-(3alpha,7alpha,12alpha-triacetoxy-5-beta-cholanamido)-1,3,4-thiadiazole-2-sulfonamide
-
weak inhibition
5-(3alpha,7alpha,12alpha-trihydroxy-5-beta-cholanamido)-1,3,4-thiadiazole-2-sulfonamide
-
weak inhibition
5-[[5-(4-methoxy-2-nitrophenyl)furan-2-yl]methylidene]-2-thioxodihydropyrimidine-4,6(1H,5H)-dione
-
reversible, competitive inhibition versus NADP+, mixed-type versus D-glucose 6-phosphate
5alpha-androstan-16-alpha-bromo-3beta-ol-17-one
-
in vitro cell viability assays shows a LD50 of 20 microM for Trypanosoma cruzi epimastigotes
5alpha-androsten-16-alpha-bromo-3beta-ol-17-one
-
potent inhibitor, in vitro cell viability assays shows a LD50 of 12 microM for Trypanosoma cruzi epimastigotes
ammonia
-
inhibition in vitro and in vivo at sublethal concentration of 0.0022-0.0055 mM, IC50: 0.0187 mM
beta-naphthoquinone-4-sulfonic acid
-
1 mM, 68% inhibition, G6PDH-1; 1 mM, 81% inhibition, G6PDH-2
cumene hydroperoxide
-
1% residual activity after treatment with 17 mM cumene hydroperoxide at 50°C and pH 7 for 2 h
deltamethrin
-
inhibits G6PD both in vivo and in vitro, significantly inhibits activity after the 48th hour. Among pesticides, it is the most effective one, which is widely used both at homes and in agricultural fields. Deltamethrin inhibits the enzyme at very low doses, particularly in in vivo conditions, indicating that fish in natural and cultural environments are susceptible to this pesticide and that deltamethrin contaminations can be cause high mortality in fish population, which may lead to the increase in food insufficiency for increasing populations and cause disruption of ecological balance. Thus, usage of deltamethrin must be well controlled
ellagic acid
mixed type inhibitor of glucose 6-phosphate activity
guanosine hydrochloride
-
the enzyme shows a sharp loss in activity above 0.75 M, but is activated by low concentrations of 0.2 M of GdmCl
Nicotine
-
inhibits the enzyme from lung, testis, kidney, stomach, and brain, in concert with vitamin E the enzyme from testis brain and liver is inhibited, tissue-specific inhibition of 12.5-48%, overview, nicotine has no effect on enzyme from muscle, heart, and liver
peracetic acid
-
1% residual activity after treatment with 4 mM peracetic acid at 25°C and pH 7 for 15 min
quartz
-
24 h incubation with quartz particles (80 microg/cm(2)) inhibits G6PD activity by 70%. Inhibition is fully prevented by glutathione. Silica exerts on G6PD an oxidative damage
-
tert-butyl hydroperoxide
-
1% residual activity after treatment with 290 mM tert-butyl hydroperoxide at 50°C and pH 7 for 3 h
Tl+
-
201Tl solution and radiation exposure has inhibitory effects on the enzyme activity both in vivo and in vitro
Trypsin
after 2 h of trypsin digestion in the presence of 0.01 mM NADP+ the enzyme retains about 57% of its original activity
-
Urea
-
inhibition in vitro and in vivo at sublethal concentration of 0.02-0.05 mM, IC50: 0.0238 mM
vitamin E
-
in concert with nicotine the enzyme from testis brain and liver is inhibited, while the enzyme from muscle and stomach is activated, overview
ATP
-
31% inhibition at 5 mM, non-competitive inhibition with respect to D-glucose 6-phosphate
Cu2+
-
potent inhibitor, about 65% residual activity at 2 mM (isoform G6PD2), about 50% residual activity at 2 mM (isoform G6PD1)
DTT
-
1 mM, 2 h, 20% loss of activity in the first 15 min and then the enzyme activity remains steady throughout the incubation
epiandrosterone
uncompetitive inhibitor, 12% residual activity at 0.03 mM
Fe2+
-
potent inhibitor, about 10% residual activity at 5 mM (isoform G6PD2), about 30% residual activity at 5 mM (isoform G6PD1)
Fe3+
-
enzyme activities are significantly decreased in the presence of 30 and 300 ppm Fe3+
Fe3+
competitive inhibitor against the G6PD enzyme with respect to NADP+ and D-glucose 6-phosphate
glucose
-
high glucose level of 25 mM leads to a decrease in G6PD activity and protein level in islets
guanidinium hydrochloride
-
in the presence of 4 M, after 2 hours all secondary structure is lost
NADH
-
allosteric inhibition, inhibition is not reversed by NAD+, AMP, or spermidine
NADP+
-
at high concentration, dead-end ternary complexes are formed
NADPH
-
product inhibition with NADP+ or D-glucose 6-phosphate as the varying substrate
NADPH
-
when NADP+ is the varied substrate, NADPH NADPH is a competitive inhibitor both in the presence and absence of Mg2+, linear competitive inhibition. When glucose 6-phosphate is the varied substrate NADPH causes linear noncompetitive inhibition
NADPH
-
NADPH binding is important for physiological regulation of pentose phosphate pathway
p-chloromercuribenzoate
-
can be reversed 100% by dithiothreitol, partially by glutathione
RNAi
-
suppression of endogenous cytosolic G6PDH isoforms result in highly uniform defense responses and also enhanced drought tolerance and flowering
-
Zn2+
-
potent inhibitor, about 58% residual activity at 5 mM (isoform G6PD2), about 35% residual activity at 2 mM (isoform G6PD1)
additional information
Trx f1 regulates G6PDH1 activity as efficiently as Trx m1 or m4. Trx x is a very poor regulator of G6PDH activity. Trx y1 is inefficient as inhibitor but it shows high efficiency in activation. Upon illumination, a strong and fast reductive inhibition of G6PDH1 activity dependent on the presence of all the components of the Fd/Trx system
-
additional information
during 7 days of phosphate starvation, G6PD5 is continuously expressed throughout phosphate-starvation; during 7 days of phosphate starvation, transcript levels are reduced; during 7 days of phosphate starvation, transcript levels are reduced; during 7 days of phosphate starvation, transcript levels are reduced; during 7 days of phosphate starvation, transcript levels are reduced; during 7 days of phosphate starvation, transcript levels are reduced
-
additional information
during 7 days of phosphate starvation, G6PD5 is continuously expressed throughout phosphate-starvation; during 7 days of phosphate starvation, transcript levels are reduced; during 7 days of phosphate starvation, transcript levels are reduced; during 7 days of phosphate starvation, transcript levels are reduced; during 7 days of phosphate starvation, transcript levels are reduced; during 7 days of phosphate starvation, transcript levels are reduced
-
additional information
during 7 days of phosphate starvation, G6PD5 is continuously expressed throughout phosphate-starvation; during 7 days of phosphate starvation, transcript levels are reduced; during 7 days of phosphate starvation, transcript levels are reduced; during 7 days of phosphate starvation, transcript levels are reduced; during 7 days of phosphate starvation, transcript levels are reduced; during 7 days of phosphate starvation, transcript levels are reduced
-
additional information
during 7 days of phosphate starvation, G6PD5 is continuously expressed throughout phosphate-starvation; during 7 days of phosphate starvation, transcript levels are reduced; during 7 days of phosphate starvation, transcript levels are reduced; during 7 days of phosphate starvation, transcript levels are reduced; during 7 days of phosphate starvation, transcript levels are reduced; during 7 days of phosphate starvation, transcript levels are reduced
-
additional information
during 7 days of phosphate starvation, G6PD5 is continuously expressed throughout phosphate-starvation; during 7 days of phosphate starvation, transcript levels are reduced; during 7 days of phosphate starvation, transcript levels are reduced; during 7 days of phosphate starvation, transcript levels are reduced; during 7 days of phosphate starvation, transcript levels are reduced; during 7 days of phosphate starvation, transcript levels are reduced
-
additional information
during 7 days of phosphate starvation, G6PD5 is continuously expressed throughout phosphate-starvation; during 7 days of phosphate starvation, transcript levels are reduced; during 7 days of phosphate starvation, transcript levels are reduced; during 7 days of phosphate starvation, transcript levels are reduced; during 7 days of phosphate starvation, transcript levels are reduced; during 7 days of phosphate starvation, transcript levels are reduced
-
additional information
-
during 7 days of phosphate starvation, G6PD5 is continuously expressed throughout phosphate-starvation; during 7 days of phosphate starvation, transcript levels are reduced; during 7 days of phosphate starvation, transcript levels are reduced; during 7 days of phosphate starvation, transcript levels are reduced; during 7 days of phosphate starvation, transcript levels are reduced; during 7 days of phosphate starvation, transcript levels are reduced
-
additional information
-
product and dead-end inhibition studies, overview, no inhibition by AMP
-
additional information
-
less than 8% inhibition with 1,10-phenanthroline, ATP, EDTA, and iodoacetamide. Not inhibited by NAD+
-
additional information
-
hyperaldosteronism downregulates G6PD and whereby decreases GSH levels and conversely increases oxidative stress, which evokes endothelial-derived NO and impairs vascular function
-
additional information
-
fluoride-containing bioactive glasses as used in tissue engineering as well as bone repair, inhibit the pentose phosphate oxidative pathway and the glucose 6-phosphate dehydrogenase activity. The effects are ascribable to the fluoride content/release of glass powders, they are mimicked by NaF solutions and are prevented by radical scavengers dimethyl sulfoxide and tempol, by superoxide dismutase, and by glutathione, but not by apocynin
-
additional information
-
non-radioactive Tl+, Fe3+ and Cu2+ do not influence the enzyme in vitro
-
additional information
-
is not inhibited by dehydroepiandrosterone and epiandrosterone
-
additional information
-
effects of streptomycin sulfate and tetracyclin antibiotics
-
additional information
-
high-throughput screening for small-molecule inhibitors of the enzyme from Plasmodium falciparum, inhibitor evaluation, structure-activity relationship analysis, overview
-
additional information
presence of H2O2 only slightly alters enzyme activity or expression
-
additional information
-
presence of H2O2 only slightly alters enzyme activity or expression
-
additional information
-
inhibition of G6PD protects rat hearts from ischemia-reperfusion injury induced by oxidative stress
-
additional information
-
the cytosolic isoyzme is not affected by osmotic change, phosphate sequestration, or ocidative stress
-
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
5-(3alpha,12alpha-dihydroxy-5-beta-cholanamido)-1,3,4-thiadiazole-2-sulfonamide
-
weak activation
5-(3alpha,acetoxy-5-beta-cholanamido)-1,3,4-thiadiazole-2-sulfonamide
-
activates
6-aminohexanoic acid
-
marginally increases the refolding yield in the presence of Arg, and it is unable to replace Arg in promoting a high refolding yield
artesunate
-
induces a 25fold increase in glucose-6-phosphate dehydrogenase activity while there is no significant reduction in the red blood cell count. Critical role for glucose-6-phosphate dehydrogenase in the prevention of oxidative stress and lysis of red blood cells in artesunate-treated rats
ATP
-
isoform Zwf-2 is active in the presence of 3m MATP, and then only with NADP+ as a coenzyme
butanol
-
marginally increases the refolding yield in the presence of Arg, and it is unable to replace Arg in promoting a high refolding yield
cyclophilin A
-
facilitates refolding, the yield, at about 82%, is about 12% higher than without cyclophilin A. Even with 0.0004 mM cyclophilin A in the standard refolding buffer, maximal recovery takes about 7 days
-
glycerol
-
marginally increases the refolding yield in the presence of Arg, and it is unable to replace Arg in promoting a high refolding yield
guanosine hydrochloride
-
the enzyme shows a sharp loss in activity above 0.75 M, but is activated by low concentrations of 0.2 M of GdmCl
H2O2
-
H2O2, produced under salt stress, induces increased G6PDH activity and the enzymes of glutathione cycle, which concomitantly results in an increased reduced glutathione contents
Insulin
-
stimulates, in pregnant women with types 1 diabetes insulin effect on the enzyme activity is lower than in the control. In the group of pregnant women with type 2 diabetes and gestational diabetes the effect of insulin is decreased as compared to control
-
insulin-like growth factor 1
-
stimulates, in pregnant women with types 1 diabetes effect of insulin-like growth factor 1 is absent. In the group of pregnant women with type 2 diabetes and gestational diabetes the effect of insulin-like growth factor 1 is decreased as compared to control
-
L-Arg
-
L-Arg has major positive influence on refolding. L-Arg is the key player in the refolding of human G6PD, preventing the aggregation of folding intermediate
N-ethylmaleimide
-
blocking the SH group leads to activation of rBmG6PD activity
paraquat
-
activates plastidic isozymes by 10fold by direct action on the enzyme, no activation of the cytosolic isozyme
PEG 3350
-
marginally increases the refolding yield in the presence of Arg, and it is unable to replace Arg in promoting a high refolding yield
-
relaxin
-
stimulates, in pregnant women with types 1 diabetes effect of relaxin is absent. In the group of pregnant women with type 2 diabetes and gestational diabetes the effect of relaxin is somewhat higher as compared to control
-
sucrose
-
in wild-type seedlings G6PD5 and G6PD6 show small (1.7- and 2fold, respectively) but significant induction by sucrose
trehalose
-
marginally increases the refolding yield in the presence of Arg, and it is unable to replace Arg in promoting a high refolding yield
vitamin E
-
in concert with nicotine the enzyme from testis brain and liver is inhibited, while the enzyme from muscle and stomach is activated by 36% and 20%, respectively, overview
additional information
activity of G6PDH increases rapidly just after the inoculation of 10-day-old cells into fresh medium
-
additional information
activity of G6PDH increases rapidly just after the inoculation of 10-day-old cells into fresh medium
-
additional information
activity of G6PDH increases rapidly just after the inoculation of 10-day-old cells into fresh medium
-
additional information
activity of G6PDH increases rapidly just after the inoculation of 10-day-old cells into fresh medium
-
additional information
activity of G6PDH increases rapidly just after the inoculation of 10-day-old cells into fresh medium
-
additional information
activity of G6PDH increases rapidly just after the inoculation of 10-day-old cells into fresh medium
-
additional information
-
activity of G6PDH increases rapidly just after the inoculation of 10-day-old cells into fresh medium
-
additional information
oxidative activation is strictly dependent on plastidial thioredoxins that show differential efficiencies. Trx f1 regulates G6PDH1 activity as efficiently as Trx m1 or m4. Trx x is a very poor regulator of G6PDH activity. Trx y1 is inefficient as inhibitor but it shows high efficiency in activation. G6PDH activity can be recovered by transferring from light to dark, reversibility of inactivation certifying redox modulation of the enzyme. In the dark, reactivation can be enhanced by the addition of oxidants, such as oxidized dithiothreitol and H2O2
-
additional information
-
temperature and protein concentration have positive influence on refolding. Refolding at 25°C is relatively slow, taking about one week to reach the maximum activity. Final refolding yield at pH 7.5 is about 69%, similar to 71% at pH 8.0, and only slightly higher than 65% at pH 8.5. At 30°C, refolding is faster in the early stages than at 25°C, but the highest activities, achieved after 4-5 days, are 49% at pH 7.5, 53% at pH 8.0, and 48% at pH 8.5, respectively, considerably lower than at 25°C. Correct refolding is severely suppressed at 37°C, with the highest recovery yield at 13%. Recovery yields are 54%, 70%, 56%, and 33% for protein concentrations at 5 microg/ml, 10 microg/ml, 20 microg/ml and 50 microg/ml, respectively
-
additional information
-
after pathogen infection with Phytophthora nicotianae, G6PDH activity rises 2 to 3fold in leaves of a resistant, but not of a susceptible, tobacco cultivar
-
additional information
-
light and metabolizable sugars induce the cytosolic isozyme by increased de novo protein synthesis, while the plastidic isozymes are unaffected
-
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.374
2-deoxy-D-glucose 6-phosphate
-
pH 8.0, 25°C, recombinant mutant R454C, with NADP+
0.0078
D-glucose 6-phosphate
wild-type enzyme, pH 7.5, 30°C
0.00953
D-glucose 6-phosphate
-
pH 8.0, 25°C, recombinant mutant R454C, with NADP+
0.00971
D-glucose 6-phosphate
-
pH 8.0, 25°C, recombinant mutant R454H, with NADP+
0.0145
D-glucose 6-phosphate
-
pH 7.5, temperature not specified in the publication
0.0488
D-glucose 6-phosphate
-
pH 8.0, 25°C, recombinant mutant R454C, with deamino-NADP+
0.052
D-glucose 6-phosphate
-
pH 8.0, 25°C, recombinant wild-type enzyme, with NADP+
0.07
D-glucose 6-phosphate
-
pH 8.0, temperature not specified in the publication
0.073
D-glucose 6-phosphate
mutant enzyme A117S/F277I/Q324H/M381I/V443I/S470I, pH 7.5, 30°C
0.081
D-glucose 6-phosphate
mutant enzyme L99I/A117S/G225S/F277I/Q324H/M381I/V443I/S470I/A476V, pH 7.5, 30°C
0.099
D-glucose 6-phosphate
mutant enzyme A117S/Q324H/M381I/V443I/S470I, pH 7.5, 30°C
0.122
D-glucose 6-phosphate
-
pH 7.4, 37°C, with NADP+ or deamino-NADP+
0.145
D-glucose 6-phosphate
-
25°C, pH 8.0, high-level glucose-6-phosphate dehydrogenase strain DF82
0.151
D-glucose 6-phosphate
mutant enzyme A117S/Q324H/V443I/S470I, pH 7.5, 30°C
0.168
D-glucose 6-phosphate
mutant enzyme R408A, at pH 7.5 and 25°C
0.206
D-glucose 6-phosphate
-
pH 7.6, temperature not specified in the publication
0.21
D-glucose 6-phosphate
pH and temperature not specified in the publication
0.24
D-glucose 6-phosphate
-
G6PDH activity extracted from source leaves of wild-type tobacco SNN cultivars
0.25
D-glucose 6-phosphate
-
G6PDH activity extracted from source leaves of wild-type tobacco Xanthi cultivars
0.78
D-glucose 6-phosphate
-
native enzyme, pH and temperature not specified in the publication
0.86
D-glucose 6-phosphate
-
recombinant His-tagged enzyme, pH and temperature not specified in the publication
0.0156
deamino-NADP+
-
pH 8.0, 25°C, recombinant mutant R454C, with D-glucose 6-phosphate
0.00238
NADP+
-
pH 8.0, 25°C, recombinant mutant R454H, with D-glucose 6-phosphate
0.00276
NADP+
-
pH 8.0, 25°C, recombinant mutant R454C, with D-glucose 6-phosphate