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Literature summary for 1.1.1.49 extracted from

  • Adediran, S.A.
    Kinetic and thermodynamic properties of two electrophoretically similar genetic variants of human erythrocyte glucose-6-phosphate dehydrogenase (1996), Biochimie, 78, 165-170.
    View publication on PubMed

General Stability

General Stability Organism
isoform A(-) is less stable during purification than A(+) due to V68M mutation that makes it suceptible to oxidation of sulfhydryl group Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-glucose 6-phosphate + H2O + NADP+ Homo sapiens isoform A(-) shows sigmoidal enzyme-substrate interaction, isoform A(+) shows hyperbolic enzyme-substrate interaction D-gluconate 6-phosphate + NADPH + H+ D-glucose-delta-lactone 6-phosphate r

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
2 isoforms: A(+) and A(-), where A(-) is a mutant derivative of A(+) with V68M exchange
-

Purification (Commentary)

Purification (Comment) Organism
both isoforms A(-) and A(+) Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
erythrocyte
-
Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
isoform A(-) shows about ten times lower specific activity than isoform A(+) Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-glucose 6-phosphate + H2O + NADP+ isoform A(-) shows sigmoidal enzyme-substrate interaction, isoform A(+) shows hyperbolic enzyme-substrate interaction Homo sapiens D-gluconate 6-phosphate + NADPH + H+ D-glucose-delta-lactone 6-phosphate r

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.3
-
isoform A(+) Homo sapiens
9
-
isoform A(-) Homo sapiens

Cofactor

Cofactor Comment Organism Structure
NADP+ isoform A(-) has a higher affinity for NADP+ Homo sapiens