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Literature summary for 1.1.1.49 extracted from

  • Niehaus, W.G.; Mallett, T.C.
    Purification and characterization of glucose-6-phosphate dehydrogenase from Cryptococcus neoformans: identification as "nothing dehydrogenase" (1994), Arch. Biochem. Biophys., 313, 304-309.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
2'-phosphoadenosine 5'-diphosphoribose
-
Cryptococcus neoformans
ADP
-
Cryptococcus neoformans
AMP only 2'-AMP Cryptococcus neoformans
Cd2+
-
Cryptococcus neoformans
NADPH
-
Cryptococcus neoformans
Zn2+
-
Cryptococcus neoformans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0016
-
NADP+
-
Cryptococcus neoformans
0.024
-
D-glucose 6-phosphate
-
Cryptococcus neoformans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50000
-
? * 50000 SDS-PAGE Cryptococcus neoformans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-glucose 6-phosphate + H2O + NADP+ Cryptococcus neoformans
-
D-gluconate 6-phosphate + NADPH + H+
-
?

Organic Solvent Stability

Organic Solvent Comment Organism
dimethylsulfoxide substrate glucose and 40% DMSO (v/v): 10% remaining enzyme activity in HEPES buffer, 20% remaining enzyme activity in phosphate buffer Cryptococcus neoformans

Organism

Organism UniProt Comment Textmining
Cryptococcus neoformans
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Cryptococcus neoformans

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
50
-
-
Cryptococcus neoformans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-glucose + NAD(P)+ activity only with dimethylsulfoxide added by 40% (v/v) Cryptococcus neoformans D-gluconate + NAD(P)H
-
?
D-glucose 6-phosphate + H2O + NADP+
-
Cryptococcus neoformans D-gluconate 6-phosphate + NADPH + H+
-
?

Subunits

Subunits Comment Organism
? ? * 50000 SDS-PAGE Cryptococcus neoformans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8
-
-
Cryptococcus neoformans

pH Range

pH Minimum pH Maximum Comment Organism
6.5 8.5
-
Cryptococcus neoformans

Cofactor

Cofactor Comment Organism Structure
NADP+ absolute specificity Cryptococcus neoformans