Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Hordeum vulgare |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Hordeum vulgare |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0066 | - |
NADP+ | native enzyme, pH and temperature not specified in the publication | Hordeum vulgare | |
0.015 | - |
NADP+ | recombinant His-tagged enzyme, pH and temperature not specified in the publication | Hordeum vulgare | |
0.78 | - |
D-glucose 6-phosphate | native enzyme, pH and temperature not specified in the publication | Hordeum vulgare | |
0.86 | - |
D-glucose 6-phosphate | recombinant His-tagged enzyme, pH and temperature not specified in the publication | Hordeum vulgare |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
plastid | - |
Hordeum vulgare | 9536 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
55800 | - |
- |
Hordeum vulgare |
57000 | - |
- |
Hordeum vulgare |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glucose 6-phosphate + NADP+ | Hordeum vulgare | - |
6-phospho-D-glucono-1,5-lactone + NADPH + H+ | - |
? |
Organic Solvent | Comment | Organism |
---|---|---|
dithiothreitol | the recombinant His-tagged enzyme shows 60% residual activity after 1 h at 25 mM dithiothreitol | Hordeum vulgare |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Hordeum vulgare | - |
cultivar Nure | - |
Purification (Comment) | Organism |
---|---|
Blue-Sepharose column chromatography, Resource-Q column chromatography, and HiTrap column chromatography | Hordeum vulgare |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
root | - |
Hordeum vulgare | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glucose 6-phosphate + NADP+ | - |
Hordeum vulgare | 6-phospho-D-glucono-1,5-lactone + NADPH + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 55800, calculated from amino acid sequence | Hordeum vulgare |
? | x * 57000, native enzyme, SDS-PAGE | Hordeum vulgare |
Synonyms | Comment | Organism |
---|---|---|
P2-G6PDH | - |
Hordeum vulgare |
plastidial glucose 6-phosphate dehydrogenase | - |
Hordeum vulgare |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Hordeum vulgare |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.066 | - |
NADPH | recombinant His-tagged enzyme, pH and temperature not specified in the publication | Hordeum vulgare | |
0.095 | - |
NADPH | native enzyme, pH and temperature not specified in the publication | Hordeum vulgare |