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Literature summary for 1.1.1.49 extracted from

  • Scharte, J.; Schoen, H.; Tjaden, Z.; Weis, E.; von Schaewen, A.
    Isoenzyme replacement of glucose-6-phosphate dehydrogenase in the cytosol improves stress tolerance in plants (2009), Proc. Natl. Acad. Sci. USA, 106, 8061-8066.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.1.1.49

Activating Compound

Activating Compound Comment Organism Structure
additional information after pathogen infection with Phytophthora nicotianae, G6PDH activity rises 2 to 3fold in leaves of a resistant, but not of a susceptible, tobacco cultivar Nicotiana tabacum

Application

Application Comment Organism
additional information because isoenzyme replacement of G6PDH in the cytosol of tobacco is beneficial under various kinds of cues, this strategy may be a tool to enhance stress tolerance in general Arabidopsis thaliana

Cloned(Commentary)

Cloned (Comment) Organism
into vector pET16b and expressed in a G6PDH-deficient Escherichia coli strain. Overexpression in the cytosol of the susceptible tobacco cultivar Xanthi Arabidopsis thaliana

Inhibitors

Inhibitors Comment Organism Structure
glucosamine 6-phosphate competitive G6PDH inhibitor Nicotiana tabacum
NADPH
-
 Arabidopsis thaliana
NADPH
-
 Nicotiana tabacum
RNAi suppression of endogenous cytosolic G6PDH isoforms result in highly uniform defense responses and also enhanced drought tolerance and flowering Nicotiana tabacum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.024
-
 NADP+
-
 Arabidopsis thaliana
0.041
-
 NADP+ G6PDH activity extracted from source leaves of wild-type tobacco SNN cultivars Nicotiana tabacum
0.041
-
 NADP+ G6PDH activity extracted from source leaves of wild-type tobacco Xanthi cultivars Nicotiana tabacum
0.24
-
 D-glucose 6-phosphate G6PDH activity extracted from source leaves of wild-type tobacco SNN cultivars Nicotiana tabacum
0.25
-
 D-glucose 6-phosphate G6PDH activity extracted from source leaves of wild-type tobacco Xanthi cultivars Nicotiana tabacum
0.57
-
 D-glucose 6-phosphate
-
 Arabidopsis thaliana

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol cytosolic G6PDH seems to be a crucial factor for the outcome of plant defense responses Nicotiana tabacum 5829
-
plastid
-
 Arabidopsis thaliana 9536
-

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana Q8L743
-
-
Nicotiana tabacum
-
 var. Samsun, resistant SNN cultivar and susceptible Xanthi cultivar
-

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Nicotiana tabacum
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-glucose 6-phosphate + NADP+
-
 Nicotiana tabacum D-glucono-1,5-lactone 6-phosphate + NADPH + H+
-
 ?
D-glucose 6-phosphate + NADP+
-
 Arabidopsis thaliana D-glucono-1,5-lactone 6-phosphate + NADPH + H+
-
 ?
additional information transgenic (previously susceptible) lines overexpressing G6PDH-encoding gene, displaying high NADPH tolerance engineered for cytosolic expression, show early oxidative bursts, callose deposition, and changes in metabolic parameter Nicotiana tabacum ?
-
 ?

Synonyms

Synonyms Comment Organism
G6PDH
-
 Nicotiana tabacum
G6PDH
-
 Arabidopsis thaliana

Cofactor

Cofactor Comment Organism Structure
NADP+
-
 Nicotiana tabacum
NADP+
-
 Arabidopsis thaliana

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.05
-
 NADPH G6PDH activity extracted from source leaves of wild-type tobacco Xanthi cultivars Nicotiana tabacum
0.051
-
 NADPH G6PDH activity extracted from source leaves of wild-type tobacco SNN cultivars Nicotiana tabacum
0.06
-
 NADPH
-
 Arabidopsis thaliana