Literature summary for 1.1.1.49 extracted from

Ibrahim, M.A.; Ghazy, A.H.; Salem, A.M.; Ghazy, M.A.; Abdel-Monsef, M.M.
Biochemical characterization of buffalo liver glucose-6-phosphate dehydrogenase isoforms (2015), Protein J., 34, 193-204.

Search for more literature for 1.1.1.49

Inhibitors

Inhibitors	Comment	Organism	Structure
Cu2+	potent inhibitor, about 65% residual activity at 2 mM (isoform G6PD2), about 50% residual activity at 2 mM (isoform G6PD1)	Bos taurus
Fe2+	potent inhibitor, about 10% residual activity at 5 mM (isoform G6PD2), about 30% residual activity at 5 mM (isoform G6PD1)	Bos taurus
Mn2+	about 10% residual activity at 5 mM (isoform G6PD2)	Bos taurus
NADPH	competitive inhibition	Bos taurus
Zn2+	potent inhibitor, about 58% residual activity at 5 mM (isoform G6PD2), about 35% residual activity at 2 mM (isoform G6PD1)	Bos taurus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.059	-	NADP+	isoform G6PD1, at pH 8.0, temperature not specified in the publication	Bos taurus
0.06	-	NADP+	isoform G6PD1, at pH 8.0, temperature not specified in the publication	Bos taurus

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Co2+	about 110% activity at 5 mM (isoform G6PD1)	Bos taurus
Mg2+	about 130% activity at 5 mM (isoform G6PD2), about 150% activity at 5 mM (isoform G6PD1)	Bos taurus
Mn2+	about 112% activity at 2 mM (isoform G6PD1)	Bos taurus
additional information	not influenced by Ca2+	Bos taurus
Ni2+	about 110% activity at 5 mM (isoform G6PD2), about 115% activity at 2 mM (isoform G6PD1)	Bos taurus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
28000	-	isoform G6PD1, gel filtration	Bos taurus
66000	-	isoform G6PD2, gel filtration	Bos taurus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
D-glucose 6-phosphate + NADP+	Bos taurus	-	6-phospho-D-glucono-1,5-lactone + NADPH + H+	-	?

Organism

Organism	UniProt	Comment	Textmining
Bos taurus	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
ammonium sulfate precipitation, DEAE-cellulose column chromatography, and Sephacryl S-300 gel filtration, and 2',5'-ADP Sepharose 4B column chromatography	Bos taurus

Source Tissue

Source Tissue	Comment	Organism	Textmining
liver	-	Bos taurus	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.0374	-	crude extract, at pH 8.0, temperature not specified in the publication	Bos taurus
0.916	-	isoform G6PD1, after 24.49fold purification, at pH 8.0, temperature not specified in the publication	Bos taurus
2.221	-	isoform G6PD2, after 59.38fold purification, at pH 8.0, temperature not specified in the publication	Bos taurus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
D-glucose 6-phosphate + NADP+	-	Bos taurus	6-phospho-D-glucono-1,5-lactone + NADPH + H+	-	?

Subunits

Subunits	Comment	Organism
monomer	1 * 28000, isoform G6PD1, SDS-PAGE	Bos taurus
monomer	1 * 66000, isoform G6PD2, SDS-PAGE	Bos taurus

Synonyms

Synonyms	Comment	Organism
G6PD1	isoform	Bos taurus
G6PD2	isoform	Bos taurus
glucose-6-phosphate dehydrogenase	-	Bos taurus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8	-	isoform G6PD1	Bos taurus
8.2	-	isoform G6PD2	Bos taurus

Cofactor

Cofactor	Comment	Organism	Structure
NADP+	-	Bos taurus

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.012	-	NADPH	isoform G6PD1, at pH 8.0, temperature not specified in the publication	Bos taurus
0.03	-	NADPH	isoform G6PD2, at pH 8.0, temperature not specified in the publication	Bos taurus

pI Value

Organism	Comment	pI Value Maximum	pI Value
Bos taurus	isoform G6PD2, isoelectric focusing	5.9	5.7
Bos taurus	isoform G6PD1, isoelectric focusing	7.9	7.7

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Release 2023.1 (January 2023)