Activating Compound | Comment | Organism | Structure |
---|---|---|---|
anhydrotetracyclin | induces expression of mature G6PDH1 | Arabidopsis thaliana | |
additional information | oxidative activation is strictly dependent on plastidial thioredoxins that show differential efficiencies. Trx f1 regulates G6PDH1 activity as efficiently as Trx m1 or m4. Trx x is a very poor regulator of G6PDH activity. Trx y1 is inefficient as inhibitor but it shows high efficiency in activation. G6PDH activity can be recovered by transferring from light to dark, reversibility of inactivation certifying redox modulation of the enzyme. In the dark, reactivation can be enhanced by the addition of oxidants, such as oxidized dithiothreitol and H2O2 | Arabidopsis thaliana |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli DH5alpha strain from the pASK-G6PDH1 plasmid | Arabidopsis thaliana |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | Trx f1 regulates G6PDH1 activity as efficiently as Trx m1 or m4. Trx x is a very poor regulator of G6PDH activity. Trx y1 is inefficient as inhibitor but it shows high efficiency in activation. Upon illumination, a strong and fast reductive inhibition of G6PDH1 activity dependent on the presence of all the components of the Fd/Trx system | Arabidopsis thaliana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast stroma | - |
Arabidopsis thaliana | 9570 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | Q43727 | - |
- |
Purification (Comment) | Organism |
---|---|
by affinity chromatography, to homogeneity | Arabidopsis thaliana |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
11.23 | - |
purified G6PDH1 upon reduction by reduced DTT | Arabidopsis thaliana |
20.3 | - |
purified G6PDH1 | Arabidopsis thaliana |
139.5 | - |
purified G6PDH1 upon oxidation by diamide | Arabidopsis thaliana |
Storage Stability | Organism |
---|---|
-20°C, 100 mM Tris-HCl buffer, pH 7.9, several months | Arabidopsis thaliana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glucose 6-phosphate + NADP+ | - |
Arabidopsis thaliana | D-glucono-1,5-lactone 6-phosphate + NADPH + H+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
G6PDH1 | - |
Arabidopsis thaliana |