Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.1.1.49 extracted from

  • McCarthy, J.K.; O'Brien, C.E.; Eveleigh, D.E.
    Thermostable continuous coupled assay for measuring glucose using glucokinase and glucose-6-phosphate dehydrogenase from the marine hyperthermophile Thermotoga maritima (2003), Anal. Biochem., 318, 196-203.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene gpd, expression in Escherichia coli strain BL21(DE3) as His-tagged enzyme Thermotoga maritima

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.019
-
D-glucose 6-phosphate pH 7.8, 85°C, recombinant enzyme Thermotoga maritima
0.08
-
NADP+ pH 7.8, 85°C, recombinant enzyme Thermotoga maritima

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
57500
-
about, recombinant enzyme, native PAGE Thermotoga maritima

Organism

Organism UniProt Comment Textmining
Thermotoga maritima
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Escherichia coli by nickel affinity chromatography Thermotoga maritima

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
76
-
purified recombinant enzyme, development of a coupled continuous thermostable assay method, determination at 85°C Thermotoga maritima

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-glucose 6-phosphate + NADP+
-
Thermotoga maritima D-glucono-1,5-lactone 6-phosphate + NADPH + H+
-
?

Subunits

Subunits Comment Organism
monomer 1 x 57500, recombinant enzyme, SDS-PAGE Thermotoga maritima

Synonyms

Synonyms Comment Organism
glucose-6-phosphate dehydrogenase
-
Thermotoga maritima
GPD
-
Thermotoga maritima

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
85
-
-
Thermotoga maritima

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
70 90 over 60% of maximal activity at 70°C and 90°C, recombinant enzyme Thermotoga maritima

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
recombinant enzyme Thermotoga maritima

pH Range

pH Minimum pH Maximum Comment Organism
6 8.5 recombinant enzyme Thermotoga maritima

Cofactor

Cofactor Comment Organism Structure
NADP+
-
Thermotoga maritima